Evaluation of genotoxicity induced by exposure to antineoplastic drugs in lymphocytes of oncology nurses and pharmacists

ABSTRACT The hazards of handling antineoplastic drugs have been raised and discussed in several studies. Introduction of new antineoplastics together with abuse of safety standards have contributed to the exposure risk for personnel who handle these substances. Interactions of antineoplastic drugs w...

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Vydáno v:Journal of applied toxicology Ročník 33; číslo 3; s. 196 - 201
Hlavní autoři: El-Ebiary, Ahmad A., Abuelfadl, Arwa A., Sarhan, Naglaa I.
Médium: Journal Article
Jazyk:angličtina
Vydáno: England Blackwell Publishing Ltd 01.03.2013
Wiley Subscription Services, Inc
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ISSN:0260-437X, 1099-1263, 1099-1263
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Abstract ABSTRACT The hazards of handling antineoplastic drugs have been raised and discussed in several studies. Introduction of new antineoplastics together with abuse of safety standards have contributed to the exposure risk for personnel who handle these substances. Interactions of antineoplastic drugs with biological structures vary according to the drug(s) and the individual's genetic susceptibility. This study was carried out to evaluate the genome damage induced by exposure to antineoplastic drugs in nurses (n = 20) and pharmacists (n = 18) working in the Oncology Department of Tanta Cancer Center. Thirty subjects matched in age, gender and smoking habit were selected as controls. Both chromosomal aberration analysis and micronucleus assay were used to evaluate genome damage in peripheral blood lymphocytes of the study subjects. The numbers of aberrant lymphocytes, as well as chromosomal aberration and micronuclei frequencies, were significantly increased in exposed personnel in comparison to matched controls. Compared with pharmacists, nurses showed notably higher level of chromosome damage. On the other hand, no significant difference in micronuclei frequency was observed between nurses and pharmacists. Correlation analyses pointed to the influence of age and duration of occupational exposure on the level of chromosome damage among exposed subjects. The results of this study confirmed that handling antineoplastic drugs without appropriate precautions imposed a genotoxic risk for exposed healthcare workers. These results address the need for regular biomonitoring of exposed personnel. In addition, they call attention to the need for proper implementation of intervention measures aiming to eliminate or significantly reduce worker exposure and prevent untoward biological effects. Copyright © 2011 John Wiley & Sons, Ltd. Pharmacists and nurses handling antineoplastic drugs showed significantly increased chromosomal aberration and micronuclei frequencies. Compared with pharmacists, nurses showed notably higher levels of chromosome damage, but not micronuclei frequency. Correlation analyses pointed to the influence of age and duration of occupational exposure on the level of chromosome damage among exposed subjects.
AbstractList The hazards of handling antineoplastic drugs have been raised and discussed in several studies. Introduction of new antineoplastics together with abuse of safety standards have contributed to the exposure risk for personnel who handle these substances. Interactions of antineoplastic drugs with biological structures vary according to the drug(s) and the individual's genetic susceptibility. This study was carried out to evaluate the genome damage induced by exposure to antineoplastic drugs in nurses (n=20) and pharmacists (n=18) working in the Oncology Department of Tanta Cancer Center. Thirty subjects matched in age, gender and smoking habit were selected as controls. Both chromosomal aberration analysis and micronucleus assay were used to evaluate genome damage in peripheral blood lymphocytes of the study subjects. The numbers of aberrant lymphocytes, as well as chromosomal aberration and micronuclei frequencies, were significantly increased in exposed personnel in comparison to matched controls. Compared with pharmacists, nurses showed notably higher level of chromosome damage. On the other hand, no significant difference in micronuclei frequency was observed between nurses and pharmacists. Correlation analyses pointed to the influence of age and duration of occupational exposure on the level of chromosome damage among exposed subjects. The results of this study confirmed that handling antineoplastic drugs without appropriate precautions imposed a genotoxic risk for exposed healthcare workers. These results address the need for regular biomonitoring of exposed personnel. In addition, they call attention to the need for proper implementation of intervention measures aiming to eliminate or significantly reduce worker exposure and prevent untoward biological effects. Copyright © 2011 John Wiley & Sons, Ltd. Pharmacists and nurses handling antineoplastic drugs showed significantly increased chromosomal aberration and micronuclei frequencies. Compared with pharmacists, nurses showed notably higher levels of chromosome damage, but not micronuclei frequency. Correlation analyses pointed to the influence of age and duration of occupational exposure on the level of chromosome damage among exposed subjects. [PUBLICATION ABSTRACT]
The hazards of handling antineoplastic drugs have been raised and discussed in several studies. Introduction of new antineoplastics together with abuse of safety standards have contributed to the exposure risk for personnel who handle these substances. Interactions of antineoplastic drugs with biological structures vary according to the drug(s) and the individual's genetic susceptibility. This study was carried out to evaluate the genome damage induced by exposure to antineoplastic drugs in nurses (n = 20) and pharmacists (n = 18) working in the Oncology Department of Tanta Cancer Center. Thirty subjects matched in age, gender and smoking habit were selected as controls. Both chromosomal aberration analysis and micronucleus assay were used to evaluate genome damage in peripheral blood lymphocytes of the study subjects. The numbers of aberrant lymphocytes, as well as chromosomal aberration and micronuclei frequencies, were significantly increased in exposed personnel in comparison to matched controls. Compared with pharmacists, nurses showed notably higher level of chromosome damage. On the other hand, no significant difference in micronuclei frequency was observed between nurses and pharmacists. Correlation analyses pointed to the influence of age and duration of occupational exposure on the level of chromosome damage among exposed subjects. The results of this study confirmed that handling antineoplastic drugs without appropriate precautions imposed a genotoxic risk for exposed healthcare workers. These results address the need for regular biomonitoring of exposed personnel. In addition, they call attention to the need for proper implementation of intervention measures aiming to eliminate or significantly reduce worker exposure and prevent untoward biological effects.The hazards of handling antineoplastic drugs have been raised and discussed in several studies. Introduction of new antineoplastics together with abuse of safety standards have contributed to the exposure risk for personnel who handle these substances. Interactions of antineoplastic drugs with biological structures vary according to the drug(s) and the individual's genetic susceptibility. This study was carried out to evaluate the genome damage induced by exposure to antineoplastic drugs in nurses (n = 20) and pharmacists (n = 18) working in the Oncology Department of Tanta Cancer Center. Thirty subjects matched in age, gender and smoking habit were selected as controls. Both chromosomal aberration analysis and micronucleus assay were used to evaluate genome damage in peripheral blood lymphocytes of the study subjects. The numbers of aberrant lymphocytes, as well as chromosomal aberration and micronuclei frequencies, were significantly increased in exposed personnel in comparison to matched controls. Compared with pharmacists, nurses showed notably higher level of chromosome damage. On the other hand, no significant difference in micronuclei frequency was observed between nurses and pharmacists. Correlation analyses pointed to the influence of age and duration of occupational exposure on the level of chromosome damage among exposed subjects. The results of this study confirmed that handling antineoplastic drugs without appropriate precautions imposed a genotoxic risk for exposed healthcare workers. These results address the need for regular biomonitoring of exposed personnel. In addition, they call attention to the need for proper implementation of intervention measures aiming to eliminate or significantly reduce worker exposure and prevent untoward biological effects.
The hazards of handling antineoplastic drugs have been raised and discussed in several studies. Introduction of new antineoplastics together with abuse of safety standards have contributed to the exposure risk for personnel who handle these substances. Interactions of antineoplastic drugs with biological structures vary according to the drug(s) and the individual's genetic susceptibility. This study was carried out to evaluate the genome damage induced by exposure to antineoplastic drugs in nurses ( n = 20) and pharmacists ( n = 18) working in the Oncology Department of Tanta Cancer Center. Thirty subjects matched in age, gender and smoking habit were selected as controls. Both chromosomal aberration analysis and micronucleus assay were used to evaluate genome damage in peripheral blood lymphocytes of the study subjects. The numbers of aberrant lymphocytes, as well as chromosomal aberration and micronuclei frequencies, were significantly increased in exposed personnel in comparison to matched controls. Compared with pharmacists, nurses showed notably higher level of chromosome damage. On the other hand, no significant difference in micronuclei frequency was observed between nurses and pharmacists. Correlation analyses pointed to the influence of age and duration of occupational exposure on the level of chromosome damage among exposed subjects. The results of this study confirmed that handling antineoplastic drugs without appropriate precautions imposed a genotoxic risk for exposed healthcare workers. These results address the need for regular biomonitoring of exposed personnel. In addition, they call attention to the need for proper implementation of intervention measures aiming to eliminate or significantly reduce worker exposure and prevent untoward biological effects. Copyright © 2011 John Wiley & Sons, Ltd. Pharmacists and nurses handling antineoplastic drugs showed significantly increased chromosomal aberration and micronuclei frequencies. Compared with pharmacists, nurses showed notably higher levels of chromosome damage, but not micronuclei frequency. Correlation analyses pointed to the influence of age and duration of occupational exposure on the level of chromosome damage among exposed subjects.
ABSTRACT The hazards of handling antineoplastic drugs have been raised and discussed in several studies. Introduction of new antineoplastics together with abuse of safety standards have contributed to the exposure risk for personnel who handle these substances. Interactions of antineoplastic drugs with biological structures vary according to the drug(s) and the individual's genetic susceptibility. This study was carried out to evaluate the genome damage induced by exposure to antineoplastic drugs in nurses (n = 20) and pharmacists (n = 18) working in the Oncology Department of Tanta Cancer Center. Thirty subjects matched in age, gender and smoking habit were selected as controls. Both chromosomal aberration analysis and micronucleus assay were used to evaluate genome damage in peripheral blood lymphocytes of the study subjects. The numbers of aberrant lymphocytes, as well as chromosomal aberration and micronuclei frequencies, were significantly increased in exposed personnel in comparison to matched controls. Compared with pharmacists, nurses showed notably higher level of chromosome damage. On the other hand, no significant difference in micronuclei frequency was observed between nurses and pharmacists. Correlation analyses pointed to the influence of age and duration of occupational exposure on the level of chromosome damage among exposed subjects. The results of this study confirmed that handling antineoplastic drugs without appropriate precautions imposed a genotoxic risk for exposed healthcare workers. These results address the need for regular biomonitoring of exposed personnel. In addition, they call attention to the need for proper implementation of intervention measures aiming to eliminate or significantly reduce worker exposure and prevent untoward biological effects. Copyright © 2011 John Wiley & Sons, Ltd. Pharmacists and nurses handling antineoplastic drugs showed significantly increased chromosomal aberration and micronuclei frequencies. Compared with pharmacists, nurses showed notably higher levels of chromosome damage, but not micronuclei frequency. Correlation analyses pointed to the influence of age and duration of occupational exposure on the level of chromosome damage among exposed subjects.
The hazards of handling antineoplastic drugs have been raised and discussed in several studies. Introduction of new antineoplastics together with abuse of safety standards have contributed to the exposure risk for personnel who handle these substances. Interactions of antineoplastic drugs with biological structures vary according to the drug(s) and the individual's genetic susceptibility. This study was carried out to evaluate the genome damage induced by exposure to antineoplastic drugs in nurses (n=20) and pharmacists (n=18) working in the Oncology Department of Tanta Cancer Center. Thirty subjects matched in age, gender and smoking habit were selected as controls. Both chromosomal aberration analysis and micronucleus assay were used to evaluate genome damage in peripheral blood lymphocytes of the study subjects. The numbers of aberrant lymphocytes, as well as chromosomal aberration and micronuclei frequencies, were significantly increased in exposed personnel in comparison to matched controls. Compared with pharmacists, nurses showed notably higher level of chromosome damage. On the other hand, no significant difference in micronuclei frequency was observed between nurses and pharmacists. Correlation analyses pointed to the influence of age and duration of occupational exposure on the level of chromosome damage among exposed subjects. The results of this study confirmed that handling antineoplastic drugs without appropriate precautions imposed a genotoxic risk for exposed healthcare workers. These results address the need for regular biomonitoring of exposed personnel. In addition, they call attention to the need for proper implementation of intervention measures aiming to eliminate or significantly reduce worker exposure and prevent untoward biological effects. Copyright [copy 2011 John Wiley & Sons, Ltd. Pharmacists and nurses handling antineoplastic drugs showed significantly increased chromosomal aberration and micronuclei frequencies. Compared with pharmacists, nurses showed notably higher levels of chromosome damage, but not micronuclei frequency. Correlation analyses pointed to the influence of age and duration of occupational exposure on the level of chromosome damage among exposed subjects.
The hazards of handling antineoplastic drugs have been raised and discussed in several studies. Introduction of new antineoplastics together with abuse of safety standards have contributed to the exposure risk for personnel who handle these substances. Interactions of antineoplastic drugs with biological structures vary according to the drug(s) and the individual's genetic susceptibility. This study was carried out to evaluate the genome damage induced by exposure to antineoplastic drugs in nurses (n = 20) and pharmacists (n = 18) working in the Oncology Department of Tanta Cancer Center. Thirty subjects matched in age, gender and smoking habit were selected as controls. Both chromosomal aberration analysis and micronucleus assay were used to evaluate genome damage in peripheral blood lymphocytes of the study subjects. The numbers of aberrant lymphocytes, as well as chromosomal aberration and micronuclei frequencies, were significantly increased in exposed personnel in comparison to matched controls. Compared with pharmacists, nurses showed notably higher level of chromosome damage. On the other hand, no significant difference in micronuclei frequency was observed between nurses and pharmacists. Correlation analyses pointed to the influence of age and duration of occupational exposure on the level of chromosome damage among exposed subjects. The results of this study confirmed that handling antineoplastic drugs without appropriate precautions imposed a genotoxic risk for exposed healthcare workers. These results address the need for regular biomonitoring of exposed personnel. In addition, they call attention to the need for proper implementation of intervention measures aiming to eliminate or significantly reduce worker exposure and prevent untoward biological effects.
Author El-Ebiary, Ahmad A.
Abuelfadl, Arwa A.
Sarhan, Naglaa I.
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  fullname: Sarhan, Naglaa I.
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Fucic A, Jazbec A, Mijic A, Seso-Simic D, Tomek R. 1998. Cytogenetic consequences after occupational exposure to antineoplastic drugs. Mutat. Res. 416: 59-66.
Buckton KE, Evans HJ. 1973. Methods for the Analysis of Human Chromosome Aberrations. World Health Organization: Geneva.
Burgaz S, Karahalil B, Canli Z, Terzioglu F, Ancel G, Anzion RBM, Bos RP, Huttner E. 2002. Assessment of genotoxic damage in nurses occupationally exposed to antineoplastics by the analysis of chromosomal aberrations. Hum. Exp. Toxicol. 21: 129-135.
Valanis B, Vollmer WM, Labuhn KT, Glass AG. 1993. Acute symptoms associated with antineoplastic drug handling among nurses. Cancer Nurs. 16: 288-295.
Hessel H, Radon K, Pethran A, Maisch B, Grombmair S, Sautter I, Fruhmann G. 2001. The genotoxic risk of hospital, pharmacy and medical personnel occupationally exposed to cytostatic drugs - evaluation by the micronucleus assay. Mutat. Res. 497: 101-109.
Surralles J, Natarajan AT. 1997. Human lymphocytes micronucleus assay in Europe. An international survey. Mutat. Res. 392: 165-174.
Fuchs J, Hengstler JG, Jung D, Hiltl G, Konietzko J, Oesch F. 1995. DNA damage in nurses handling antineoplastic agents. Mutat. Res. 342: 17-23.
Maluf SW, Erdtmann B. 2000. Follow up study of the genetic damage in lymphocytes of pharmacists and nurses handling antineoplastic drugs evaluated by cytokinesis-block micronuclei analysis and single cell gel electrophoresis assay. Mutat. Res. 471: 21-27.
Hagmar L, Bonassi S, Stromberg U, Brogger A, Knudsen LE, Norppa H, Reuterwall C. 1998. Chromosomal aberrations in lymphocytes predict human cancer: a report from the European Study Group on Cytogenetic Biomarkers and Health (ESCH). Cancer Res. 58: 4117-4121.
Cavallo D, Ursini CL, Perniconi B, Francesco AD, Giglio M, Rubino FM, Marinaccio A, Iavicoli S. 2005. Evaluation of genotoxic effects induced by exposure to antineoplastic drugs in lymphocytes and exfoliated buccal cells of oncology nurses and pharmacy employees. Mutat. Res. 587: 45-51.
Cavallo D, Ursini CL, Omodeo-Sale E, Iavicoli S. 2007. Micronucleus induction and FISH analysis in buccal cells and lymphocytes of nurses administering antineoplastic drugs. Mutat. Res. 628: 11-18.
Rekhadevi PV, Sailaja N, Chandrasekhar M, Mahboob M, Rahman MF, Grover P. 2007. Genotoxicity assessment in oncology nurses handling anti-neoplastic drugs. Mutagenesis 22: 395-401.
Bonassi S, Znaor A, Ceppi M, Lando C, Chang WP, Holland N, Kirsch-Volders M, Zeiger E, Ban S, Barale R, Bigatti MP, Bolognesi C, Cebulska-Wasilewska A, Fabianova E, Fucic A, Hagmar L, Joksic G, Martelli A, Migliore L, Mirkova E, Scarfi MR, Zijno A, Norppa H, Fenech M. 2007. An increased micronucleus frequency in peripheral blood lymphocytes predicts the risk of cancer in humans. Carcinogenesis 28: 625-631.
Ensslin AS, Huber R, Pethran A, Rommelt H, Schierl R, Kulka U, Fruhmann G. 1997. Biological monitoring of hospital pharmacy personnel occupationally exposed to cytostatic drugs: urinary excretion and cytogenetics study. Int. Arch. Occup. Environ. Health 70: 205-208.
Yoshida J, Kosaka H, Tomioka K, Kumagai S. 2006. Genotoxic risks to nurses from contamination of the work environment with antineoplastic drugs in Japan. J. Occup. Health 48: 517-522.
Kevekordes S, Gebel TW, Hellwig M, Dames W, Dunkelberg H. 1998. Human effect monitoring in cases of occupational exposure to antineoplastic drugs: a method comparison. Occup. Environ. Med. 55: 145-149.
Preston RJ, Dean BS, Galloway S, Holden H, McFee AF, Shelby M. 1987. Mammalian in vivo cytogenetic assays: analysis of chromosome aberrations in bone marrow cells. Mutat. Res. 189: 157-165.
Laffon B, Teixeira JP, Silva S, Loureiro J, Torres J, Pa´saro E, Me´ndez J, Mayan O. 2005. Genotoxic effects in a population of nurses handling antineoplastic drugs, and relationship with genetic polymorphisms in DNA repair enzymes. Am. J. Ind. Med. 48: 128-136.
Fenech M, Morely AA. 1985. Measurement of micronuclei in lymphocytes. Mutat. Res. 147: 29-36.
Jakab MG, Major J, Tompa A. 2001. Follow-up genotoxicological monitoring of nurses handling antineoplastic drugs. J. Toxicol. Environ. Health 62: 307-318.
Berwick M, Vineis P. 2000. Markers of DNA repair and susceptibility to cancer in humans: an epidemiologic review. J. Natl. Cancer Inst. 92: 874-897.
Brumen V, Horvat D. 1996. Work environment influence on cytostatics induced genotoxicity in oncologic nurses. Am. J. Ind. Med. 30: 67-71.
Oestreicher U, Stephan G, Glatzel M. 1990. Chromosome and SCE analysis in peripheral lymphocytes of persons occupationally exposed to cytostatic drugs handled with and without use of safety covers. Mutat. Res. 242: 271-277.
Rombaldi F, Cassini C, Salvador M, Saffi J, Erdtmann B. 2009. Occupational risk assessment of genotoxicity and oxidative stress in workers handling anti-neoplastic drugs during a working week. Mutagenesis 24: 143-148.
Turci R, Sottani C, Spagnoli G, Minoia C. 2003. Biological and environmental monitoring of hospital personnel exposed to antineoplastic agents: a review of analytical methods. J. Chromatogr. 789: 169-209.
Pilger A, Kohler I, Stettner H, Mader RM, Rizovski B, Terkola R, Diem E, Franz-Hainzl E, Konnaris C, Valic E, Rudiger HW. 2000. Long-term monitoring of sister chromatid exchanges and micronucleus frequencies in pharmacy personnel occupationally exposed to cytostatic drugs. Int. Arch. Occup. Environ. Health 73: 442-448.
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SSID ssj0009928
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Snippet ABSTRACT The hazards of handling antineoplastic drugs have been raised and discussed in several studies. Introduction of new antineoplastics together with...
The hazards of handling antineoplastic drugs have been raised and discussed in several studies. Introduction of new antineoplastics together with abuse of...
SourceID proquest
pubmed
crossref
wiley
istex
SourceType Aggregation Database
Index Database
Enrichment Source
Publisher
StartPage 196
SubjectTerms Aberration
Abnormal Karyotype
Adult
Antineoplastic Agents - adverse effects
antineoplastic drug
chromosomal aberration
Chromosomes
Damage
DNA Damage
Drugs
Exposure
Female
genotoxicity
Health Facility Environment
Humans
Karyotyping
lymphocyte
Lymphocytes
Lymphocytes - drug effects
Lymphocytes - pathology
Male
Materials handling
Micronuclei, Chromosome-Defective - chemically induced
micronucleus assay
Micronucleus Tests
Mutagens - adverse effects
nurse
Nurses
Nursing Staff, Hospital
Occupational Exposure - adverse effects
Occupational hazards
pharmacist
Pharmacists
Prescription drugs
Toxicity
Title Evaluation of genotoxicity induced by exposure to antineoplastic drugs in lymphocytes of oncology nurses and pharmacists
URI https://api.istex.fr/ark:/67375/WNG-QL80RDGF-K/fulltext.pdf
https://onlinelibrary.wiley.com/doi/abs/10.1002%2Fjat.1735
https://www.ncbi.nlm.nih.gov/pubmed/21935972
https://www.proquest.com/docview/1434888920
https://www.proquest.com/docview/1282046119
https://www.proquest.com/docview/1439222704
https://www.proquest.com/docview/1753537624
Volume 33
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