Rapid label-free visual assay for the detection and quantification of viral RNA using peptide nucleic acid (PNA) and gold nanoparticles (AuNPs)

•Simple and easy to use visual assay for viral diagnosis.•It is rapid and label-free assay with pen-side diagnostic applications.•The assay shows high sensitivity and specificity with ability to detect single nucleotide difference at target site visually.•Method demonstrates successful quantificatio...

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Veröffentlicht in:	Analytica chimica acta Jg. 795; S. 1 - 7
Hauptverfasser:	Joshi, Vinay G., Chindera, Kantaraja, Singh, Arvind Kumar, Sahoo, Aditya P., Dighe, Vikas D., Thakuria, Dimpal, Tiwari, Ashok K., Kumar, Satish
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	Netherlands Elsevier B.V 17.09.2013
Schlagworte:	Avian orthoavulavirus 1 cell culture colorimetry Genotype genotyping Gold - chemistry Gold nanoparticles (AuNPs) hemagglutination Label-free assay Metal Nanoparticles - chemistry monitoring mutation nanogold Newcastle disease virus Newcastle disease virus - genetics Peptide nucleic acid (PNA) Peptide Nucleic Acids - chemical synthesis Peptide Nucleic Acids - chemistry Phenotype quantitative polymerase chain reaction RNA RNA, Viral - analysis Spectrophotometry vaccines Viral RNA quantification viruses Visual RNA detection Visual RNA detection Peptide nucleic acid (PNA) Label-free assay Viral RNA quantification Gold nanoparticles (AuNPs)
ISSN:	0003-2670, 1873-4324, 1873-4324
Online-Zugang:	Volltext
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