An exon-biased biophysical approach and NMR spectroscopy define the secondary structure of a conserved helical element within the HOTAIR long non-coding RNA

[Display omitted] •-Structure determination is challenging for lncRNAs that contain multiple splice isoforms.•-An exon-biased biophysical approach that includes NMR and chemical probing was developed to identify homogenous conformations within the exons of HOTAIR.•-HOTAIR exon 4 contains a well-defi...

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Bibliographic Details
Published in:	Journal of structural biology Vol. 213; no. 2; p. 107728
Main Authors:	Abzhanova, Ainur, Hirschi, Alexander, Reiter, Nicholas J.
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 01.06.2021
Subjects:	Chemical probing Exons Humans Magnetic Resonance Spectroscopy NMR spectroscopy Non-coding RNA Nucleic Acid Conformation RNA Folding RNA secondary structure RNA structure prediction RNA, Long Noncoding - chemistry RNA, Long Noncoding - genetics Ultracentrifugation hHOTAIR Chemical probing NMR lncRNA RNA secondary structure R-scape RNA structure prediction NMR spectroscopy Non-coding RNA
ISSN:	1047-8477, 1095-8657, 1095-8657
Online Access:	Get full text
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