In vitro vs. in vivo tape stripping: Validation of the porcine ear model and penetration assessment of novel sucrose stearate emulsions

In vitro tape stripping on porcine ear skin and in vivo tape stripping on human forearm skin yield comparable results. Porcine ear skin is frequently used as a substitute for human skin in dermatological research and is especially useful for tape stripping experiments where the penetration of active...

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Vydané v:	European journal of pharmaceutics and biopharmaceutics Ročník 80; číslo 3; s. 604 - 614
Hlavní autori:	Klang, Victoria, Schwarz, Julia C., Lenobel, Barbara, Nadj, Martina, Auböck, Josef, Wolzt, Michael, Valenta, Claudia
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	Amsterdam Elsevier B.V 01.04.2012 Elsevier
Predmet:	Adolescent Adult Animals Biological and medical sciences Curcumin - administration & dosage Ear, External Emulsions - administration & dosage Female Fluorescein - administration & dosage Forearm General pharmacology Humans Hydrogel, Polyethylene Glycol Dimethacrylate - administration & dosage Male Medical sciences Nanoemulsion Nanotechnology - methods Pharmaceutical technology. Pharmaceutical industry Pharmacology. Drug treatments Porcine ears Skin - metabolism Skin Absorption - drug effects Skin penetration Sucrose - administration & dosage Sucrose - analogs & derivatives Sucrose stearate Surface Properties - drug effects Swine Tape stripping Young Adult SC Porcine ears Skin penetration TEWL NIR-densitometry Sucrose stearate Nanoemulsion Tape stripping Validation Evaluation Pharmaceutical technology Sucrose Stearate Adhesive tape In vitro Pig In vivo Vertebrata Mammalia Emulsion Penetration Animal Ear Skin Artiodactyla Ungulata
ISSN:	0939-6411, 1873-3441, 1873-3441
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Popis
Shrnutí:	In vitro tape stripping on porcine ear skin and in vivo tape stripping on human forearm skin yield comparable results. Porcine ear skin is frequently used as a substitute for human skin in dermatological research and is especially useful for tape stripping experiments where the penetration of active substances into the uppermost skin layers is investigated. However, certain differences between the surface properties of these skin types exist, and reports on the comparability of tape stripping data obtained in vitro using porcine ear skin and data obtained in vivo on human forearm skin are scarce. Thus, we performed comparative tape stripping experiments in which the skin penetration of curcumin and fluorescein sodium from conventional microemulsions and hydrogels was investigated. In this context, the skin penetration potential of novel semi-solid macroemulsions and fluid nanoemulsions based on sucrose stearate was evaluated as well. The removed corneocytes were quantified by NIR-densitometry using recent correlation data for human and porcine proteins. The trends observed for the skin penetration into porcine ear skin were highly representative for the in vivo situation on human skin, confirming that the porcine ear is an excellent in vitro model for tape stripping experiments. Moreover, the validity of the NIR-densitometric approach for the quantification of both human and porcine stratum corneum proteins was confirmed in this study for the first time.
Bibliografia:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0939-6411 1873-3441 1873-3441
DOI:	10.1016/j.ejpb.2011.11.009