Zebrafish Retinal Ganglion Cells Asymmetrically Encode Spectral and Temporal Information across Visual Space
In vertebrate vision, the tetrachromatic larval zebrafish permits non-invasive monitoring and manipulating of neural activity across the nervous system in vivo during ongoing behavior. However, despite a perhaps unparalleled understanding of links between zebrafish brain circuits and visual behavior...
Uložené v:
| Vydané v: | Current biology Ročník 30; číslo 15; s. 2927 |
|---|---|
| Hlavní autori: | , , , , , , |
| Médium: | Journal Article |
| Jazyk: | English |
| Vydavateľské údaje: |
England
03.08.2020
|
| Predmet: | |
| ISSN: | 1879-0445, 1879-0445 |
| On-line prístup: | Zistit podrobnosti o prístupe |
| Tagy: |
Pridať tag
Žiadne tagy, Buďte prvý, kto otaguje tento záznam!
|
| Abstract | In vertebrate vision, the tetrachromatic larval zebrafish permits non-invasive monitoring and manipulating of neural activity across the nervous system in vivo during ongoing behavior. However, despite a perhaps unparalleled understanding of links between zebrafish brain circuits and visual behaviors, comparatively little is known about what their eyes send to the brain via retinal ganglion cells (RGCs). Major gaps in knowledge include any information on spectral coding and information on potentially critical variations in RGC properties across the retinal surface corresponding with asymmetries in the statistics of natural visual space and behavioral demands. Here, we use in vivo two-photon imaging during hyperspectral visual stimulation as well as photolabeling of RGCs to provide a functional and anatomical census of RGCs in larval zebrafish. We find that RGCs' functional and structural properties differ across the eye and include a notable population of UV-responsive On-sustained RGCs that are only found in the acute zone, likely to support visual prey capture of UV-bright zooplankton. Next, approximately half of RGCs display diverse forms of color opponency, including many that are driven by a pervasive and slow blue-Off system-far in excess of what would be required to satisfy traditional models of color vision. In addition, most information on spectral contrast was intermixed with temporal information. Taken together, our results suggest that zebrafish RGCs send a diverse and highly regionalized time-color code to the brain. |
|---|---|
| AbstractList | In vertebrate vision, the tetrachromatic larval zebrafish permits non-invasive monitoring and manipulating of neural activity across the nervous system in vivo during ongoing behavior. However, despite a perhaps unparalleled understanding of links between zebrafish brain circuits and visual behaviors, comparatively little is known about what their eyes send to the brain via retinal ganglion cells (RGCs). Major gaps in knowledge include any information on spectral coding and information on potentially critical variations in RGC properties across the retinal surface corresponding with asymmetries in the statistics of natural visual space and behavioral demands. Here, we use in vivo two-photon imaging during hyperspectral visual stimulation as well as photolabeling of RGCs to provide a functional and anatomical census of RGCs in larval zebrafish. We find that RGCs' functional and structural properties differ across the eye and include a notable population of UV-responsive On-sustained RGCs that are only found in the acute zone, likely to support visual prey capture of UV-bright zooplankton. Next, approximately half of RGCs display diverse forms of color opponency, including many that are driven by a pervasive and slow blue-Off system-far in excess of what would be required to satisfy traditional models of color vision. In addition, most information on spectral contrast was intermixed with temporal information. Taken together, our results suggest that zebrafish RGCs send a diverse and highly regionalized time-color code to the brain. In vertebrate vision, the tetrachromatic larval zebrafish permits non-invasive monitoring and manipulating of neural activity across the nervous system in vivo during ongoing behavior. However, despite a perhaps unparalleled understanding of links between zebrafish brain circuits and visual behaviors, comparatively little is known about what their eyes send to the brain via retinal ganglion cells (RGCs). Major gaps in knowledge include any information on spectral coding and information on potentially critical variations in RGC properties across the retinal surface corresponding with asymmetries in the statistics of natural visual space and behavioral demands. Here, we use in vivo two-photon imaging during hyperspectral visual stimulation as well as photolabeling of RGCs to provide a functional and anatomical census of RGCs in larval zebrafish. We find that RGCs' functional and structural properties differ across the eye and include a notable population of UV-responsive On-sustained RGCs that are only found in the acute zone, likely to support visual prey capture of UV-bright zooplankton. Next, approximately half of RGCs display diverse forms of color opponency, including many that are driven by a pervasive and slow blue-Off system-far in excess of what would be required to satisfy traditional models of color vision. In addition, most information on spectral contrast was intermixed with temporal information. Taken together, our results suggest that zebrafish RGCs send a diverse and highly regionalized time-color code to the brain.In vertebrate vision, the tetrachromatic larval zebrafish permits non-invasive monitoring and manipulating of neural activity across the nervous system in vivo during ongoing behavior. However, despite a perhaps unparalleled understanding of links between zebrafish brain circuits and visual behaviors, comparatively little is known about what their eyes send to the brain via retinal ganglion cells (RGCs). Major gaps in knowledge include any information on spectral coding and information on potentially critical variations in RGC properties across the retinal surface corresponding with asymmetries in the statistics of natural visual space and behavioral demands. Here, we use in vivo two-photon imaging during hyperspectral visual stimulation as well as photolabeling of RGCs to provide a functional and anatomical census of RGCs in larval zebrafish. We find that RGCs' functional and structural properties differ across the eye and include a notable population of UV-responsive On-sustained RGCs that are only found in the acute zone, likely to support visual prey capture of UV-bright zooplankton. Next, approximately half of RGCs display diverse forms of color opponency, including many that are driven by a pervasive and slow blue-Off system-far in excess of what would be required to satisfy traditional models of color vision. In addition, most information on spectral contrast was intermixed with temporal information. Taken together, our results suggest that zebrafish RGCs send a diverse and highly regionalized time-color code to the brain. |
| Author | Bear, John Roberts, Paul A Baden, Tom Zhou, Mingyi Semmelhack, Julie Janiak, Filip K Yoshimatsu, Takeshi |
| Author_xml | – sequence: 1 givenname: Mingyi surname: Zhou fullname: Zhou, Mingyi organization: School of Life Sciences, University of Sussex, Brighton BN19QG, UK – sequence: 2 givenname: John surname: Bear fullname: Bear, John organization: School of Life Sciences, University of Sussex, Brighton BN19QG, UK; Hong Kong University of Science and Technology, Hong Kong – sequence: 3 givenname: Paul A surname: Roberts fullname: Roberts, Paul A organization: School of Life Sciences, University of Sussex, Brighton BN19QG, UK – sequence: 4 givenname: Filip K surname: Janiak fullname: Janiak, Filip K organization: School of Life Sciences, University of Sussex, Brighton BN19QG, UK – sequence: 5 givenname: Julie surname: Semmelhack fullname: Semmelhack, Julie organization: Hong Kong University of Science and Technology, Hong Kong – sequence: 6 givenname: Takeshi surname: Yoshimatsu fullname: Yoshimatsu, Takeshi organization: School of Life Sciences, University of Sussex, Brighton BN19QG, UK – sequence: 7 givenname: Tom surname: Baden fullname: Baden, Tom email: t.baden@sussex.ac.uk organization: School of Life Sciences, University of Sussex, Brighton BN19QG, UK; Institute for Ophthalmic Research, University of Tübingen, Tübingen 72076, Germany. Electronic address: t.baden@sussex.ac.uk |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/32531283$$D View this record in MEDLINE/PubMed |
| BookMark | eNpNkE9LxDAQxYOsuH_0A3iRHr20JmnSNsdlWdeFBcFdPXgpaTLVLmlSk_aw394uKggD7w3zmwczczSxzgJCtwQnBJPs4ZiooUoopjjBfCx-gWakyEWMGeOTf36K5iEcMSa0ENkVmqaUp6NPZ8i8Q-Vl3YTP6AX6xkoTbaT9MI2z0QqMCdEynNoWet8oacwpWlvlNET7DlTvR1paHR2g7dy52dra-Vb2522pvAshemvCME72nVRwjS5raQLc_OoCvT6uD6unePe82a6Wu1gxTvo4ywUWkEsQWsqK6QLTvC4IKMGo0lgorguSZoSlnFRasrQawYpqnHPF85rRBbr_ye28-xog9GXbBDVeIy24IZSUESqKIidiRO9-0aFqQZedb1rpT-Xfh-g3cZVr6Q |
| CitedBy_id | crossref_primary_10_1016_j_neuron_2020_07_002 crossref_primary_10_1016_j_cub_2021_01_030 crossref_primary_10_1007_s12274_024_6754_7 crossref_primary_10_3390_ijms222111750 crossref_primary_10_1038_s41559_023_02291_7 crossref_primary_10_7554_eLife_89996_4 crossref_primary_10_1016_j_devcel_2024_07_005 crossref_primary_10_3390_ijms252212215 crossref_primary_10_1186_s13024_023_00655_y crossref_primary_10_1371_journal_pgen_1011879 crossref_primary_10_1038_s41559_024_02404_w crossref_primary_10_1038_s41467_023_41032_z crossref_primary_10_1016_j_cub_2021_09_047 crossref_primary_10_1371_journal_pgen_1011139 crossref_primary_10_3389_fnins_2022_1010302 crossref_primary_10_1016_j_cub_2025_07_007 crossref_primary_10_1242_dev_202754 crossref_primary_10_1186_s12915_020_00903_3 crossref_primary_10_1242_dev_201008 crossref_primary_10_1038_s41586_024_08518_2 crossref_primary_10_1016_j_neuron_2020_12_003 crossref_primary_10_1242_dev_200938 crossref_primary_10_1016_j_cobeha_2024_101391 crossref_primary_10_1016_j_cub_2021_01_088 crossref_primary_10_1038_s41598_023_36099_z crossref_primary_10_1016_j_jes_2021_11_008 crossref_primary_10_1038_s41598_024_67262_9 crossref_primary_10_1038_s41583_025_00932_3 crossref_primary_10_3389_fncir_2023_1087993 crossref_primary_10_1242_jeb_243907 crossref_primary_10_1126_sciadv_adp7075 crossref_primary_10_1146_annurev_vision_102122_104700 crossref_primary_10_1016_j_cub_2023_05_069 crossref_primary_10_7554_eLife_89996 crossref_primary_10_1152_jn_00082_2021 crossref_primary_10_1016_j_cub_2021_04_053 |
| ContentType | Journal Article |
| Copyright | Copyright © 2020 The Author(s). Published by Elsevier Inc. All rights reserved. |
| Copyright_xml | – notice: Copyright © 2020 The Author(s). Published by Elsevier Inc. All rights reserved. |
| DBID | CGR CUY CVF ECM EIF NPM 7X8 |
| DOI | 10.1016/j.cub.2020.05.055 |
| DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic |
| DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic |
| DatabaseTitleList | MEDLINE MEDLINE - Academic |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: 7X8 name: MEDLINE - Academic url: https://search.proquest.com/medline sourceTypes: Aggregation Database |
| DeliveryMethod | no_fulltext_linktorsrc |
| Discipline | Biology |
| EISSN | 1879-0445 |
| ExternalDocumentID | 32531283 |
| Genre | Research Support, Non-U.S. Gov't Journal Article |
| GrantInformation_xml | – fundername: Biotechnology and Biological Sciences Research Council grantid: BB/R014817/1 – fundername: Medical Research Council grantid: MC_PC_15071 |
| GroupedDBID | --- --K -DZ -~X 0R~ 1RT 1~5 2WC 4.4 457 4G. 53G 5GY 62- 6J9 7-5 AAEDT AAEDW AAFWJ AAKRW AALRI AAMRU AAVLU AAXUO AAYWO ABDGV ABJNI ABMAC ACGFO ACGFS ACVFH ADBBV ADCNI ADEZE ADVLN AEFWE AENEX AEUPX AEXQZ AFPUW AFTJW AGCQF AGHSJ AGKMS AGUBO AIGII AITUG AKAPO AKBMS AKRWK AKYEP ALMA_UNASSIGNED_HOLDINGS AMRAJ APXCP AZFZN BAWUL CGR CS3 CUY CVF DIK DU5 E3Z EBS ECM EFKBS EIF F5P FCP FDB FIRID IHE IXB J1W JIG LX5 M3Z M41 NPM O-L O9- OK1 P2P ROL RPZ SCP SDG SES SSZ TR2 7X8 |
| ID | FETCH-LOGICAL-c451t-67909e7ae9daab4d8027f81ec942cd09c5d813614351bda43b9dab2d075c57f42 |
| IEDL.DBID | 7X8 |
| ISICitedReferencesCount | 39 |
| ISICitedReferencesURI | http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000555598100005&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| ISSN | 1879-0445 |
| IngestDate | Sun Sep 28 06:59:28 EDT 2025 Mon Jul 21 06:02:18 EDT 2025 |
| IsDoiOpenAccess | false |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 15 |
| Keywords | 2P imaging retina larval zebrafish retinal ganglion cells UV-vision prey capture color vision |
| Language | English |
| License | Copyright © 2020 The Author(s). Published by Elsevier Inc. All rights reserved. |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c451t-67909e7ae9daab4d8027f81ec942cd09c5d813614351bda43b9dab2d075c57f42 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| OpenAccessLink | http://www.cell.com/article/S0960982220307429/pdf |
| PMID | 32531283 |
| PQID | 2412988719 |
| PQPubID | 23479 |
| ParticipantIDs | proquest_miscellaneous_2412988719 pubmed_primary_32531283 |
| PublicationCentury | 2000 |
| PublicationDate | 2020-08-03 |
| PublicationDateYYYYMMDD | 2020-08-03 |
| PublicationDate_xml | – month: 08 year: 2020 text: 2020-08-03 day: 03 |
| PublicationDecade | 2020 |
| PublicationPlace | England |
| PublicationPlace_xml | – name: England |
| PublicationTitle | Current biology |
| PublicationTitleAlternate | Curr Biol |
| PublicationYear | 2020 |
| SSID | ssj0012896 |
| Score | 2.5277095 |
| Snippet | In vertebrate vision, the tetrachromatic larval zebrafish permits non-invasive monitoring and manipulating of neural activity across the nervous system in vivo... In vertebrate vision, the tetrachromatic larval zebrafish permits non-invasive monitoring and manipulating of neural activity across the nervous system in vivo... |
| SourceID | proquest pubmed |
| SourceType | Aggregation Database Index Database |
| StartPage | 2927 |
| SubjectTerms | Animals Behavior, Animal Brain - physiology Color Vision - physiology Photic Stimulation Retina - cytology Retina - embryology Retinal Ganglion Cells - physiology Spatial Processing - physiology Ultraviolet Rays Zebrafish - physiology |
| Title | Zebrafish Retinal Ganglion Cells Asymmetrically Encode Spectral and Temporal Information across Visual Space |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/32531283 https://www.proquest.com/docview/2412988719 |
| Volume | 30 |
| WOSCitedRecordID | wos000555598100005&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| hasFullText | |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1LS8NAEF7UKnjx_agvVvAaTHY3jz1JKVYvlqJVipewr2AhTapphf57ZzcpPQmCl1yygbAzO4_9ZuZD6IaqDNxMZjyiaOSxLKOeFMSW_YGdjCg4bKUd2UTc7yejER80F25VU1a5tInOUOtS2TvyW_A0hMOJCPjd9NOzrFEWXW0oNNZRi0IoY7U6Hq1QBEgmXHdRElsAgIVLVNPVd6m5hPSQ-G5wZxj-HmE6T9Pb_e8_7qGdJsbEnVop9tGaKQ7QVs06uThE-bsFi7Nx9YGfbcMzLH0Qtpu3LHDX5HmFO9ViMrFUWyDAfIHvC9v4ji1Vvb0XwaLQeFiPtMpx089k5YuF87n4bVzN4c0LpOPmCL327ofdR68hXfAUC4OZF8Xc5yYWhmshJNMJ5K1ZEhjFGVHa5yrUSQAyhDArkFowKmGhJBpCDxXGGSPHaKMoC3OKMDjImKokETGXjCkqfWHApBjJTMRAd9roermNKSi1RSpEYcp5la42so1Oalmk03r6RkoJmA0Iis7-8PU52rYidgV79AK1MjjS5hJtqu_ZuPq6ctoCz_7g6QeRjsq- |
| linkProvider | ProQuest |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Zebrafish+Retinal+Ganglion+Cells+Asymmetrically+Encode+Spectral+and+Temporal+Information+across+Visual+Space&rft.jtitle=Current+biology&rft.au=Zhou%2C+Mingyi&rft.au=Bear%2C+John&rft.au=Roberts%2C+Paul+A&rft.au=Janiak%2C+Filip+K&rft.date=2020-08-03&rft.issn=1879-0445&rft.eissn=1879-0445&rft.volume=30&rft.issue=15&rft.spage=2927&rft_id=info:doi/10.1016%2Fj.cub.2020.05.055&rft.externalDBID=NO_FULL_TEXT |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1879-0445&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1879-0445&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1879-0445&client=summon |