Reference interval for type III procollagen (PIIINP) using the Advia centaur PIIINP assay in adults and elderly

The amino-terminal peptide of type III procollagen (PIIINP) is a byproduct of type III collagen synthesis that exhibits promise as a biomarker of fibrosis, specifically in monitoring hepatic fibrosis in methotrexate treated patients. The Advia Centaur ® PIIINP assay is developed for track-based auto...

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Bibliographic Details
Published in:Scandinavian journal of clinical and laboratory investigation Vol. 81; no. 8; pp. 649 - 652
Main Authors: Pettersson-Pablo, Paul, Samyn, Dieter, Wasim, Jamil, Vink, Martin
Format: Journal Article
Language:English
Published: England Taylor & Francis 01.12.2021
Subjects:
Adult
Aged
Aged, 80 and over
biomarker
Biomarkers
Collagen Type III
Female
fibrosis
Humans
Male
methotrexate
Middle Aged
Peptide Fragments
Procollagen
reference ranges
Reference Values
Young Adult
methotrexate
reference ranges
fibrosis
Procollagen
biomarker
ISSN:0036-5513, 1502-7686, 1502-7686
Online Access:Get full text
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Summary:The amino-terminal peptide of type III procollagen (PIIINP) is a byproduct of type III collagen synthesis that exhibits promise as a biomarker of fibrosis, specifically in monitoring hepatic fibrosis in methotrexate treated patients. The Advia Centaur ® PIIINP assay is developed for track-based automated laboratory systems and is suitable for large volume analysis. Reference intervals in children and younger adults have been published previously. Here we measured PIIINP to determine reference ranges, specifically including elderly patients, for whom such are currently lacking. Samples were collected from subjects ranging from 20 to 98 years of age. Blood donors and clinical samples from primary care patients were used for reference interval calculation. Samples were analysed using the Advia Centaur ® PIIINP assay. After exclusion of samples high in alanine transaminase (AST), aspartate transaminase (ALT), and C-reactive protein (CRP) 386 samples were used in the reference interval calculation. We determined the following reference interval for the Advia Centaur ® PIIINP assay: the lower limit of the reference interval (2.5% percentile with 95% CI) was 4.42 (4.20-4.65) µg/L and the upper limit of the reference interval (97.5% percentile 95% CI) 16.0 (15.04-17.02) µg/L.No significant differences in mean PIIINP concentrations were found between men and women. While differing mean PIIINP concentrations were seen among subjects in different age groups, the differences were small and partitioning of reference range was determined not to be necessary.
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ISSN:0036-5513
1502-7686
1502-7686
DOI:10.1080/00365513.2021.2001045