Potential pitfalls in skin permeation experiments: Influence of experimental factors and subsequent data evaluation

There is a growing demand for skin penetration and permeation data considering toxicological and potential drug delivery aspects for an increasing number of substances. Although there are official guidelines available, results from different skin diffusion studies are often inconsistent and sometime...

Celý popis

Uložené v:
Podrobná bibliografia
Vydané v:European journal of pharmaceutics and biopharmaceutics Ročník 72; číslo 2; s. 324 - 331
Hlavní autori: Henning, Andreas, Schaefer, Ulrich F., Neumann, Dirk
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: Amsterdam Elsevier B.V 01.06.2009
Elsevier
Predmet:
ISSN:0939-6411, 1873-3441, 1873-3441
On-line prístup:Získať plný text
Tagy: Pridať tag
Žiadne tagy, Buďte prvý, kto otaguje tento záznam!
Abstract There is a growing demand for skin penetration and permeation data considering toxicological and potential drug delivery aspects for an increasing number of substances. Although there are official guidelines available, results from different skin diffusion studies are often inconsistent and sometimes even controversial. The aim of our study is to address and to investigate the influence of experimental parameters as well as mathematical problems for subsequent evaluation of the permeation raw data. To create a reliable database diffusion experiments across human stratum corneum were performed under highly standardized conditions. The experimental data were evaluated using linear and non-linear regression analysis to determine the influence on the permeability coefficient and the lag-time. Additionally, the influence of two critical experimental parameters, temperature and unstirred water layers, on the permeability was investigated in silico. Based on our results we suggest that the influence of temperature on the permeability coefficient is small compared to the effect of other experimental parameters. Thickness of unstirred water layers has a tremendous effect on the permeation and may lead to underestimation of the permeability by more than 90%. Non-linear regression analysis seems to be superior compared to linear algorithms hence is advisable for evaluation of the experimental data. Our findings may help to optimize the experimental set-up and to reduce total costs for future skin diffusion testing. With regards to EU’s REACH-Initiative this will also help to create more and reliable data on safety issues of industrial materials.
AbstractList There is a growing demand for skin penetration and permeation data considering toxicological and potential drug delivery aspects for an increasing number of substances. Although there are official guidelines available, results from different skin diffusion studies are often inconsistent and sometimes even controversial. The aim of our study is to address and to investigate the influence of experimental parameters as well as mathematical problems for subsequent evaluation of the permeation raw data. To create a reliable database diffusion experiments across human stratum corneum were performed under highly standardized conditions. The experimental data were evaluated using linear and non-linear regression analysis to determine the influence on the permeability coefficient and the lag-time. Additionally, the influence of two critical experimental parameters, temperature and unstirred water layers, on the permeability was investigated in silico. Based on our results we suggest that the influence of temperature on the permeability coefficient is small compared to the effect of other experimental parameters. Thickness of unstirred water layers has a tremendous effect on the permeation and may lead to underestimation of the permeability by more than 90%. Non-linear regression analysis seems to be superior compared to linear algorithms hence is advisable for evaluation of the experimental data. Our findings may help to optimize the experimental set-up and to reduce total costs for future skin diffusion testing. With regards to EU's REACH-Initiative this will also help to create more and reliable data on safety issues of industrial materials.
There is a growing demand for skin penetration and permeation data considering toxicological and potential drug delivery aspects for an increasing number of substances. Although there are official guidelines available, results from different skin diffusion studies are often inconsistent and sometimes even controversial. The aim of our study is to address and to investigate the influence of experimental parameters as well as mathematical problems for subsequent evaluation of the permeation raw data. To create a reliable database diffusion experiments across human stratum corneum were performed under highly standardized conditions. The experimental data were evaluated using linear and non-linear regression analysis to determine the influence on the permeability coefficient and the lag-time. Additionally, the influence of two critical experimental parameters, temperature and unstirred water layers, on the permeability was investigated in silico. Based on our results we suggest that the influence of temperature on the permeability coefficient is small compared to the effect of other experimental parameters. Thickness of unstirred water layers has a tremendous effect on the permeation and may lead to underestimation of the permeability by more than 90%. Non-linear regression analysis seems to be superior compared to linear algorithms hence is advisable for evaluation of the experimental data. Our findings may help to optimize the experimental set-up and to reduce total costs for future skin diffusion testing. With regards to EU's REACH-Initiative this will also help to create more and reliable data on safety issues of industrial materials.There is a growing demand for skin penetration and permeation data considering toxicological and potential drug delivery aspects for an increasing number of substances. Although there are official guidelines available, results from different skin diffusion studies are often inconsistent and sometimes even controversial. The aim of our study is to address and to investigate the influence of experimental parameters as well as mathematical problems for subsequent evaluation of the permeation raw data. To create a reliable database diffusion experiments across human stratum corneum were performed under highly standardized conditions. The experimental data were evaluated using linear and non-linear regression analysis to determine the influence on the permeability coefficient and the lag-time. Additionally, the influence of two critical experimental parameters, temperature and unstirred water layers, on the permeability was investigated in silico. Based on our results we suggest that the influence of temperature on the permeability coefficient is small compared to the effect of other experimental parameters. Thickness of unstirred water layers has a tremendous effect on the permeation and may lead to underestimation of the permeability by more than 90%. Non-linear regression analysis seems to be superior compared to linear algorithms hence is advisable for evaluation of the experimental data. Our findings may help to optimize the experimental set-up and to reduce total costs for future skin diffusion testing. With regards to EU's REACH-Initiative this will also help to create more and reliable data on safety issues of industrial materials.
Author Schaefer, Ulrich F.
Neumann, Dirk
Henning, Andreas
Author_xml – sequence: 1
  givenname: Andreas
  surname: Henning
  fullname: Henning, Andreas
  organization: Saarland University, Department of Biopharmaceutics and Pharmaceutical Technology, Saarbruecken, Germany
– sequence: 2
  givenname: Ulrich F.
  surname: Schaefer
  fullname: Schaefer, Ulrich F.
  organization: Saarland University, Department of Biopharmaceutics and Pharmaceutical Technology, Saarbruecken, Germany
– sequence: 3
  givenname: Dirk
  surname: Neumann
  fullname: Neumann, Dirk
  email: d.neumann@bioinf.uni-sb.de
  organization: Saarland University, Centre for Bioinformatics Saar, Saarbruecken, Germany
BackLink http://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21549715$$DView record in Pascal Francis
https://www.ncbi.nlm.nih.gov/pubmed/18761087$$D View this record in MEDLINE/PubMed
BookMark eNp9kU1vFSEUholpY2-rf8CFYaO7mcIA82HcmKbVJk10oWtyhjkkXLnMCExT_73c3mtjXHQD4fC85-s9JydhDkjIG85qznh7ua1xu4x1w1hfs64uoRdkw_tOVEJKfkI2bBBD1UrOz8h5SlvGmOxU_5KcFajlrO82JH2bM4bswNPFZQveJ-oCTT_LsWDcIWQ3B4oP5eF2hUwf6G2wfsVgkM72n5-SwoLJc0wUwkTTOib8VbhMJ8hA8R78-pjtFTkthRK-Pt4X5MfN9ferL9Xd18-3V5_uKiMVy9XIhWUGbDuNZoRJMTRGCYFsQEAmlGqUNUqNYBphTdf1CsQkJVrko5Qjigvy_pB3iXNpJGW9c8mg9xBwXpNuu0YOjWAFfHsE13GHk17KQBB_679rKsC7IwDJgLcRgnHpiWu4kkPHVeH6A2finFJEq43LjzPnCM5rzvTeOb3Ve-f03jnNOl1CRdr8J33q4jnRx4MIyxrvHUadjNs7M7mIJutpds_J_wBNWbVw
CitedBy_id crossref_primary_10_1159_000499477
crossref_primary_10_1016_S1773_2247_11_50004_8
crossref_primary_10_1111_j_1365_2885_2012_01380_x
crossref_primary_10_1007_s11095_012_0777_6
crossref_primary_10_1208_s12249_014_0130_y
crossref_primary_10_1080_10739149_2024_2368200
crossref_primary_10_1016_j_ejpb_2022_12_010
crossref_primary_10_1517_17425255_2015_1063615
crossref_primary_10_1016_j_ejps_2015_02_018
crossref_primary_10_1016_j_ijpharm_2012_05_012
crossref_primary_10_1159_000351936
crossref_primary_10_1016_j_jconrel_2010_08_014
crossref_primary_10_1007_s00204_022_03299_x
crossref_primary_10_1016_j_jddst_2020_101926
crossref_primary_10_1208_s12249_010_9522_9
crossref_primary_10_1111_exd_12058
crossref_primary_10_1111_j_1365_2885_2011_01302_x
crossref_primary_10_1111_ics_12656
crossref_primary_10_3109_03639045_2012_694590
crossref_primary_10_1080_17425247_2021_2010702
crossref_primary_10_1016_j_ejpb_2011_01_011
crossref_primary_10_4155_tde_15_20
crossref_primary_10_1016_j_ijpharm_2022_122114
crossref_primary_10_1080_15287394_2012_688489
crossref_primary_10_3390_pharmaceutics12020152
crossref_primary_10_1007_s13346_021_01089_z
crossref_primary_10_1016_j_ijpharm_2011_11_037
ContentType Journal Article
Copyright 2008 Elsevier B.V.
2009 INIST-CNRS
Copyright_xml – notice: 2008 Elsevier B.V.
– notice: 2009 INIST-CNRS
DBID AAYXX
CITATION
IQODW
CGR
CUY
CVF
ECM
EIF
NPM
7X8
DOI 10.1016/j.ejpb.2008.07.016
DatabaseName CrossRef
Pascal-Francis
Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
MEDLINE - Academic
DatabaseTitle CrossRef
MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
MEDLINE - Academic
DatabaseTitleList MEDLINE

MEDLINE - Academic
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: 7X8
  name: MEDLINE - Academic
  url: https://search.proquest.com/medline
  sourceTypes: Aggregation Database
DeliveryMethod fulltext_linktorsrc
Discipline Pharmacy, Therapeutics, & Pharmacology
EISSN 1873-3441
EndPage 331
ExternalDocumentID 18761087
21549715
10_1016_j_ejpb_2008_07_016
S0939641108002981
Genre Research Support, Non-U.S. Gov't
Journal Article
GroupedDBID ---
--K
--M
.~1
0R~
1B1
1RT
1~.
1~5
29G
4.4
457
4G.
53G
5GY
5VS
7-5
71M
8P~
AABNK
AACTN
AAEDT
AAEDW
AAIAV
AAIKJ
AAKOC
AALRI
AAOAW
AAQFI
AAQXK
AATCM
AAXUO
AAYOK
ABFNM
ABFRF
ABGSF
ABJNI
ABMAC
ABUDA
ABXDB
ABYKQ
ABZDS
ACDAQ
ACGFO
ACIUM
ACRLP
ADBBV
ADEZE
ADMUD
ADUVX
AEBSH
AEFWE
AEHWI
AEKER
AENEX
AFKWA
AFTJW
AFXIZ
AGHFR
AGRDE
AGUBO
AGYEJ
AIEXJ
AIKHN
AITUG
AJBFU
AJOXV
ALCLG
ALMA_UNASSIGNED_HOLDINGS
AMFUW
AMRAJ
ASPBG
AVWKF
AXJTR
AZFZN
BKOJK
BLXMC
C45
CS3
DOVZS
DU5
EBS
EFJIC
EFLBG
EJD
EO8
EO9
EP2
EP3
FDB
FEDTE
FGOYB
FIRID
FNPLU
FYGXN
G-Q
GBLVA
HVGLF
HZ~
IHE
J1W
KOM
M41
MO0
N9A
O-L
O9-
OAUVE
OGGZJ
OVD
OZT
P-8
P-9
P2P
PC.
Q38
R2-
RIG
ROL
RPZ
SDF
SDG
SES
SEW
SPCBC
SSP
SSU
SSZ
T5K
TEORI
UNMZH
~G-
9DU
AATTM
AAXKI
AAYWO
AAYXX
ABWVN
ACLOT
ACRPL
ACVFH
ADCNI
ADNMO
AEIPS
AEUPX
AFJKZ
AFPUW
AGQPQ
AIGII
AIIUN
AKBMS
AKRWK
AKYEP
ANKPU
APXCP
CITATION
EFKBS
~HD
AGCQF
AGRNS
BNPGV
IQODW
SSH
CGR
CUY
CVF
ECM
EIF
NPM
7X8
ID FETCH-LOGICAL-c450t-b13f0caf6dbcbad50ecc533e09eae035525fc55bac23fc7785a3d44efe1b44be3
ISICitedReferencesCount 30
ISICitedReferencesURI http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000267000800007&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
ISSN 0939-6411
1873-3441
IngestDate Sun Sep 28 02:10:25 EDT 2025
Thu Apr 03 07:02:02 EDT 2025
Mon Jul 21 09:17:01 EDT 2025
Tue Nov 18 22:36:37 EST 2025
Sat Nov 29 02:14:10 EST 2025
Fri Feb 23 02:28:03 EST 2024
IsPeerReviewed true
IsScholarly true
Issue 2
Keywords Lag-time
Temperature
D
Permeation
MW
D app
UWL
BSA
OECD
Human skin preparation
t lag
HSA
Unstirred water layer
Modelling
PSA
Water
Human
Evaluation
Pharmaceutical technology
Modeling
Preparation
Skin
Language English
License https://www.elsevier.com/tdm/userlicense/1.0
CC BY 4.0
LinkModel OpenURL
MergedId FETCHMERGED-LOGICAL-c450t-b13f0caf6dbcbad50ecc533e09eae035525fc55bac23fc7785a3d44efe1b44be3
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
PMID 18761087
PQID 67249230
PQPubID 23479
PageCount 8
ParticipantIDs proquest_miscellaneous_67249230
pubmed_primary_18761087
pascalfrancis_primary_21549715
crossref_citationtrail_10_1016_j_ejpb_2008_07_016
crossref_primary_10_1016_j_ejpb_2008_07_016
elsevier_sciencedirect_doi_10_1016_j_ejpb_2008_07_016
PublicationCentury 2000
PublicationDate 2009-06-01
PublicationDateYYYYMMDD 2009-06-01
PublicationDate_xml – month: 06
  year: 2009
  text: 2009-06-01
  day: 01
PublicationDecade 2000
PublicationPlace Amsterdam
PublicationPlace_xml – name: Amsterdam
– name: Netherlands
PublicationTitle European journal of pharmaceutics and biopharmaceutics
PublicationTitleAlternate Eur J Pharm Biopharm
PublicationYear 2009
Publisher Elsevier B.V
Elsevier
Publisher_xml – name: Elsevier B.V
– name: Elsevier
References Mewes, Raus, Bernd, Zoeller, Saettler, Graf (bib39) 2007; 20
S.W. Collier, R.L. Bronaugh, Expression of Absorption Data: In vitro Percutaneous Absorption Principles, Fundamentals and Applications, CRC Press, 1989.
Clowes, Scott, Heylings (bib30) 1994; 8
Cordero, Alarcon, Escribano, Obach, Domenech (bib11) 1997; 86
Weast (bib17) 1970
Kandarova, Liebsch, Gerner, Schmidt, Genschern, Traue, Spielmann (bib37) 2005; 33
Thong, Zhai, Maibach (bib40) 2007; 20
OECD, Guideline 428: Skin Absorption: In vitro Method, OECD Paris, 2004.
Kemppainen, Reifenrath (bib28) 1990
Ben-Shabat, Baruch, Sintov (bib45) 2007; 33
Barrer, Barrie (bib41) 1962; 58
Bronaugh, Wester, Bucks, Maibach, Sarason (bib33) 1990; 28
Kligman, Christophers (bib7) 1963; 88
Harrison, Watkinson, Green, Hadgraft, Brain (bib10) 1996; 13
Wagner, Kostka, Lehr, Schaefer (bib8) 2000; 17
Tojo (bib24) 1985; 24
Avdeef, Nielsen, Tsinman (bib23) 2004; 22
OECD, Guideline 427: Skin Absorption: In vivo Methods, OECD Paris, 2004.
Netzlaff, Lehr, Wertz, Schaefer (bib38) 2005; 60
Aqil, Ahad, Sultana, Ali (bib44) 2007; 12
Huber, Von Heydebreck, Sültmann, Poustka, Vingron (bib50) 2002; 18
Henning, Neumann, Kostka, Lehr, Schaefer (bib6) 2008; 21
Hadgraft, Ridout (bib26) 1987; 39
Cross, Anissimov, Magnusson, Roberts (bib48) 2003; 120
US-EPA, Dermal exposure assessment: principles and applications, US Environmental Protection Agency, 1992.
Talreja, Kleene, Pickens, Wang, Kasting (bib20) 2001; 3
Akomeah, Martin, Brown (bib52) 2007; 96
Wagner, Kostka, Lehr, Schaefer (bib32) 2002; 118
Crank (bib9) 1975
Flynn (bib1) 1990
Borras-Blasco, Diez-Sales, Lopez, Herraez-Dominguez (bib12) 2004; 269
Yang, Ding, Gao (bib46) 2007; 28
Williams, Cornwell, Barry (bib51) 1992; 86
D.A. Stringer, ECETOC Monograph No. 20: Percutaneous Absorption, European Centre for Ecotoxicology and Toxicology of Chemicals (ECETOC), 1993.
Bronaugh, Maibach (bib34) 1985; 84
R.O. Potts, M.L. Francoeur, Lipid biophysics of water loss through the skin, in: Proceedings of the National Academy of Sciences of the United States of America 87, 1990, pp. 3871–3873.
Shaikh, Ademola, Maibach (bib42) 1996; 9
Potts, Guy (bib19) 1992; 9
Mitragotri, Blankschtein, Langer (bib21) 1997; 86
Carslaw, Jaeger (bib15) 1959
Scott, Carmichael, Huckle, Needham, Savage (bib31) 1993; 31
USP, The dissolution procedure: development and validation (1092), The United States Pharmacopeial Convention, 2008.
Diez-Sales, Copovi, Casabo, Herraez (bib47) 1991; 77
Schaefer-Korting, Bock, Gamer, Haberland, Haltner-Ukomadu, Kaca, Kamp, Kietzmann, Korting, Kraechter, Lehr, Liebsch, Mehling, Netzlaff, Niedorf, Ruebbelke, Schaefer, Schmidt, Schreiber, Schroeder, Spielmann, Vuia (bib36) 2006; 34
Frum, Khan, Sefcik, Rouse, Eccleston, Meidan (bib53) 2007; 336
Moser, Kriwet, Naik, Kalia, Guy (bib49) 2001; 52
Smith (bib16) 1985
Karlsson, Artursson (bib22) 1991; 71
Majumdar, Thomas, Wasdo, Sloan (bib13) 2007; 329
OECD, Guidance Document No.28: Guidance Document for the Conduct of Skin absorption studies, OECD Paris, 2004.
Johnson, Blankschtein, Langer (bib18) 1997; 86
Thomas, Majumdar, Wasdo, Majumdar, Sloan (bib14) 2007; 339
Netzlaff, Kaca, Bock, Haltner-Ukomadu, Meiers, Lehr, Schaefer (bib54) 2007; 66
H. Schaefer, T. Redelmeier, Skin barrier – Principles of Percutaneous Absorption, Karger, 1996.
References_xml – volume: 86
  start-page: 503
  year: 1997
  end-page: 508
  ident: bib11
  article-title: A comparative study of the transdermal penetration of a series of nonsteroidal antiinflammatory drugs
  publication-title: Journal of Pharmaceutical Sciences
– reference: OECD, Guidance Document No.28: Guidance Document for the Conduct of Skin absorption studies, OECD Paris, 2004.
– volume: 9
  start-page: 663
  year: 1992
  end-page: 669
  ident: bib19
  article-title: Predicting skin permeability
  publication-title: Pharmaceutical Research
– volume: 33
  start-page: 1169
  year: 2007
  end-page: 1175
  ident: bib45
  article-title: Conjugates of unsaturated fatty acids with propylene glycol as potentially less-irritant skin penetration enhancers
  publication-title: Drug Development and Industrial Pharmacy
– volume: 86
  start-page: 69
  year: 1992
  end-page: 77
  ident: bib51
  article-title: On the non-Gaussian distribution of human skin permeabilities
  publication-title: International Journal of Pharmaceutics
– volume: 3
  year: 2001
  ident: bib20
  article-title: Visualization of the lipid barrier and measurement of lipid pathlength in human stratum corneum
  publication-title: AAPS Pharmscience
– volume: 52
  start-page: 103
  year: 2001
  end-page: 112
  ident: bib49
  article-title: Passive skin penetration enhancement and its quantification in vitro
  publication-title: European Journal of Pharmaceutics and Biopharmaceutics
– volume: 21
  start-page: 81
  year: 2008
  end-page: 88
  ident: bib6
  article-title: Influence of human skin specimens consisting of different skin layers on the result of in vitro permeation experiments
  publication-title: Skin Pharmacology and Physiology
– volume: 329
  start-page: 25
  year: 2007
  end-page: 36
  ident: bib13
  article-title: The effect of water solubility of solutes on their flux through human skin in vitro
  publication-title: International Journal of Pharmaceutics
– volume: 336
  start-page: 140
  year: 2007
  end-page: 147
  ident: bib53
  article-title: Towards a correlation between drug properties and in vitro transdermal flux variability
  publication-title: International Journal of Pharmaceutics
– volume: 120
  start-page: 589
  year: 2003
  end-page: 591
  ident: bib48
  article-title: Bovine-serum-albumin-containing receptor phase better predicts transdermal absorption parameters for lipophilic compounds
  publication-title: Journal of Investigative Dermatology
– volume: 58
  start-page: 2473
  year: 1962
  end-page: 2483
  ident: bib41
  article-title: Permeation through a membrane with mixed boundary conditions
  publication-title: Transactions of the Faraday Society
– volume: 77
  start-page: 1
  year: 1991
  end-page: 11
  ident: bib47
  article-title: A modelistic approach showing the importance of the stagnant aqueous layers in in vitro diffusion studies, and in vitro–in vivo correlations
  publication-title: International Journal of Pharmaceutics
– volume: 31
  start-page: 523
  year: 1993
  end-page: 529
  ident: bib31
  article-title: Methods for measuring dermal penetration of pesticides
  publication-title: Food and Chemical Toxicology
– volume: 17
  start-page: 1475
  year: 2000
  end-page: 1481
  ident: bib8
  article-title: Drug distribution in human skin using two different in vitro test systems: comparison with in vivo data
  publication-title: Pharmaceutical Research
– reference: OECD, Guideline 427: Skin Absorption: In vivo Methods, OECD Paris, 2004.
– volume: 33
  start-page: 351
  year: 2005
  end-page: 367
  ident: bib37
  article-title: The EpiDerm test protocol for the upcoming ECVAM validation study on in vitro skin irritation tests - An assessment of the performance of the optimised test
  publication-title: ATLA Alternatives to Laboratory Animals
– volume: 66
  start-page: 127
  year: 2007
  end-page: 134
  ident: bib54
  article-title: Permeability of the reconstructed human epidermis model Episkin
  publication-title: European Journal of Pharmaceutics and Biopharmaceutics
– reference: D.A. Stringer, ECETOC Monograph No. 20: Percutaneous Absorption, European Centre for Ecotoxicology and Toxicology of Chemicals (ECETOC), 1993.
– reference: US-EPA, Dermal exposure assessment: principles and applications, US Environmental Protection Agency, 1992.
– year: 1959
  ident: bib15
  article-title: Conduction of Heat in Solids
– volume: 96
  start-page: 824
  year: 2007
  end-page: 834
  ident: bib52
  article-title: Variability in human skin permeability in vitro: comparing penetrants with different physicochemical properties
  publication-title: Journal of Pharmaceutical Sciences
– volume: 118
  start-page: 540
  year: 2002
  end-page: 544
  ident: bib32
  article-title: Human skin penetration of flufenamic acid: in vivo/in vitro correlation (deeper skin layers) for skin samples from the same subject
  publication-title: Journal of Investigative Dermatology
– volume: 86
  start-page: 1162
  year: 1997
  end-page: 1172
  ident: bib18
  article-title: Evaluation of solute permeation through the stratum corneum: lateral bilayer diffusion as the primary transport mechanism
  publication-title: Journal of Pharmaceutical Sciences
– reference: R.O. Potts, M.L. Francoeur, Lipid biophysics of water loss through the skin, in: Proceedings of the National Academy of Sciences of the United States of America 87, 1990, pp. 3871–3873.
– year: 1990
  ident: bib28
  article-title: Methods for Skin Absorption
– year: 1975
  ident: bib9
  article-title: The Mathematics of Diffusion
– volume: 88
  start-page: 702
  year: 1963
  end-page: 705
  ident: bib7
  article-title: Preparation of isolated sheets of human stratum corneum
  publication-title: Archives of Dermatology
– volume: 13
  start-page: 542
  year: 1996
  end-page: 546
  ident: bib10
  article-title: The relative effect of azone
  publication-title: Pharmaceutical Research
– volume: 28
  start-page: 369
  year: 1990
  end-page: 373
  ident: bib33
  article-title: In vivo percutaneous absorption of fragrance ingredients in rhesus monkeys and humans
  publication-title: Food and Chemical Toxicology
– reference: H. Schaefer, T. Redelmeier, Skin barrier – Principles of Percutaneous Absorption, Karger, 1996.
– volume: 34
  start-page: 283
  year: 2006
  end-page: 294
  ident: bib36
  article-title: Reconstructed human epidermis for skin absorption testing: results of the German prevalidation study
  publication-title: ATLA Alternatives to Laboratory Animals
– start-page: 93
  year: 1990
  end-page: 127
  ident: bib1
  article-title: Physicochemical determinants of skin absorption
  publication-title: Principles of Route to Route Extrapolation for Risk Assessment
– volume: 86
  start-page: 1190
  year: 1997
  end-page: 1192
  ident: bib21
  article-title: An explanation for the variation of the sonophoretic transdermal transport enhancement from drug to drug
  publication-title: Journal of Pharmaceutical Sciences
– volume: 8
  start-page: 827
  year: 1994
  end-page: 830
  ident: bib30
  article-title: Skin absorption: flow-through or static diffusion cells
  publication-title: Toxicology In Vitro
– volume: 60
  start-page: 167
  year: 2005
  end-page: 178
  ident: bib38
  article-title: The human epidermis models EpiSkin
  publication-title: European Journal of Pharmaceutics and Biopharmaceutics
– volume: 18
  year: 2002
  ident: bib50
  article-title: Variance stabilization applied to microarray data calibration and to the quantification of differential expression
  publication-title: Bioinformatics
– volume: 71
  start-page: 55
  year: 1991
  end-page: 64
  ident: bib22
  article-title: A method for the determination of cellular permeability coefficients and aqueous boundary layer thickness in monolayers of intestinal epithelial (Caco-2) cells grown in permeable filter chambers
  publication-title: International Journal of Pharmaceutics
– volume: 84
  start-page: 180
  year: 1985
  end-page: 183
  ident: bib34
  article-title: Percutaneous absorption of nitroaromatic compounds: in vivo and in vitro studies in the human and monkey
  publication-title: Journal of Investigative Dermatology
– year: 1970
  ident: bib17
  article-title: CRC Handbook of Chemistry and Physics
– volume: 39
  start-page: 149
  year: 1987
  end-page: 156
  ident: bib26
  article-title: Development of model membranes for percutaneous absorption measurements. I. Isopropyl myristate
  publication-title: International Journal of Pharmaceutics
– reference: USP, The dissolution procedure: development and validation (1092), The United States Pharmacopeial Convention, 2008.
– reference: S.W. Collier, R.L. Bronaugh, Expression of Absorption Data: In vitro Percutaneous Absorption Principles, Fundamentals and Applications, CRC Press, 1989.
– reference: OECD, Guideline 428: Skin Absorption: In vitro Method, OECD Paris, 2004.
– volume: 269
  start-page: 121
  year: 2004
  end-page: 129
  ident: bib12
  article-title: A mathematical approach to predicting the percutaneous absorption enhancing effect of sodium lauryl sulphate
  publication-title: International Journal of Pharmaceutics
– volume: 20
  start-page: 272
  year: 2007
  end-page: 282
  ident: bib40
  article-title: Percutaneous penetration enhancers: an overview
  publication-title: Skin Pharmacology and Physiology
– year: 1985
  ident: bib16
  article-title: Numerical Solution of Partial Differential Equations: Finite Difference Methods
– volume: 28
  start-page: 1252
  year: 2007
  end-page: 1254
  ident: bib46
  article-title: Effects of penetration enhancers on skin permeation behavior of fluoxetine hydrochloride in vitro
  publication-title: Academic Journal of Second Military Medical University
– volume: 20
  start-page: 85
  year: 2007
  end-page: 95
  ident: bib39
  article-title: Elastin expression in a newly developed full-thickness skin equivalent
  publication-title: Skin Pharmacology and Physiology
– volume: 24
  start-page: 368
  year: 1985
  end-page: 373
  ident: bib24
  article-title: Hydrodynamic characteristics of an in vitro drug permeation cell
  publication-title: Industrial and Engineering Chemistry Fundamentals
– volume: 9
  start-page: 274
  year: 1996
  end-page: 280
  ident: bib42
  article-title: Effects of freezing and azide treatment of in vitro human skin on the flux and metabolism of 8-methoxypsoralen
  publication-title: Skin Pharmacology
– volume: 339
  start-page: 157
  year: 2007
  end-page: 167
  ident: bib14
  article-title: The effect of water solubility of solutes on their flux through human skin in vitro: an extended Flynn database fitted to the Roberts–Sloan equation
  publication-title: International Journal of Pharmaceutics
– volume: 22
  start-page: 365
  year: 2004
  end-page: 374
  ident: bib23
  article-title: PAMPA – A drug absorption in vitro model: 11. Matching the in vivo unstirred water layer thickness by individual-well stirring in microtitre plates
  publication-title: European Journal of Pharmaceutical Sciences
– volume: 12
  start-page: 1061
  year: 2007
  end-page: 1067
  ident: bib44
  article-title: Status of terpenes as skin penetration enhancers
  publication-title: Drug Discovery Today
SSID ssj0004758
Score 2.091364
Snippet There is a growing demand for skin penetration and permeation data considering toxicological and potential drug delivery aspects for an increasing number of...
SourceID proquest
pubmed
pascalfrancis
crossref
elsevier
SourceType Aggregation Database
Index Database
Enrichment Source
Publisher
StartPage 324
SubjectTerms Algorithms
Biological and medical sciences
Diffusion
General pharmacology
Human skin preparation
Humans
Lag-time
Linear Models
Medical sciences
Modelling
Permeability
Permeation
Pharmaceutical technology. Pharmaceutical industry
Pharmacology. Drug treatments
Skin Absorption
Temperature
Unstirred water layer
Title Potential pitfalls in skin permeation experiments: Influence of experimental factors and subsequent data evaluation
URI https://dx.doi.org/10.1016/j.ejpb.2008.07.016
https://www.ncbi.nlm.nih.gov/pubmed/18761087
https://www.proquest.com/docview/67249230
Volume 72
WOSCitedRecordID wos000267000800007&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
journalDatabaseRights – providerCode: PRVESC
  databaseName: Elsevier SD Freedom Collection Journals 2021
  customDbUrl:
  eissn: 1873-3441
  dateEnd: 99991231
  omitProxy: false
  ssIdentifier: ssj0004758
  issn: 0939-6411
  databaseCode: AIEXJ
  dateStart: 19970101
  isFulltext: true
  titleUrlDefault: https://www.sciencedirect.com
  providerName: Elsevier
link http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV07b9swECacpEOBoui7TluXQ5HFkaEHaUrdgiJB0yEwUAfwJpA0hchxZcNSguSX5e_1KFKindZBM3QhDNrUw_fxeDx-d4fQl9jnWaSGsSfCTHlkypmXMEE8RhRLZDyMwimvi02ws7N4MklGnc5dEwtzPWdFEd_cJMv_KmroA2Hr0NlHiLu9KHTAZxA6tCB2aP9J8KNFpRlAOsIqrzI-n9eM1_Kypouvflkb0WX2rzlxp02tEm08bmT9bwry1A520DI19brqa2bpWqrwrQ5-a-wuL5zr3FxL5IuNTueUrYsotWRL3n7zUxP8MwOx8zno74v-ycA5s_VphFGhuQ0_apwZiSNdNV7JKPGGxOpfq6BZuAbEcE3bRib82i7cNvTrjzXBuCdmAzVbCkueZQM_-EsC7nsLY0tXbJhws1Rfw1buZCl07aC9kNEE1One0enx5IcLyWV1adj2fWy8lqEW3n-SbTbRsyUvYaZmpsTK9j1QbQuNX6DndhODjwz4XqKOKl6hg5ER5-0hHrugvvIQH-CRy49--xqVLUJxg1CcF1gjFDuE4jWEfsUtPvEiw-v4xBafGDCFHT6xxid2-HyDzk-Ox9--e7b2hycJ9StPBFHmS54Np0IKPqU-qBrYmSg_UVz5YCSHNJOUCi7DKJOMxZRHU0IAhYEgRKjoLdotFoV6j7AvQwV2KYkEiYk-CYaxilLOpAoCaLooaP79VNrE-Lo-yzzdLvcu6rdjliYtzIO_po1QU2vYGoM1BYw-OK63gYD2VqFOrMgC2kWfG0iksCzosz5eqMVVmQ6ZTgUa-V30ziDFPSbYP4Efs_1HvcIH9NTN149ot1pdqU_oibyu8nLVQztsEvfsHPgN-xvtag
linkProvider Elsevier
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Potential+pitfalls+in+skin+permeation+experiments%3A+Influence+of+experimental+factors+and+subsequent+data+evaluation&rft.jtitle=European+journal+of+pharmaceutics+and+biopharmaceutics&rft.au=Henning%2C+Andreas&rft.au=Schaefer%2C+Ulrich+F.&rft.au=Neumann%2C+Dirk&rft.date=2009-06-01&rft.issn=0939-6411&rft.volume=72&rft.issue=2&rft.spage=324&rft.epage=331&rft_id=info:doi/10.1016%2Fj.ejpb.2008.07.016&rft.externalDBID=n%2Fa&rft.externalDocID=10_1016_j_ejpb_2008_07_016
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0939-6411&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0939-6411&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0939-6411&client=summon