Combined deletion of Bap1, Nf2, and Cdkn2ab causes rapid onset of malignant mesothelioma in mice

We have generated mouse models of malignant mesothelioma (MM) based upon disruption of the Bap1, Nf2, and Cdkn2ab tumor suppressor loci in various combinations as also frequently observed in human MM. Inactivation of all three loci in the mesothelial lining of the thoracic cavity leads to a highly a...

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Published in:The Journal of experimental medicine Vol. 217; no. 6
Main Authors: Badhai, Jitendra, Pandey, Gaurav Kumar, Song, Ji-Ying, Krijgsman, Oscar, Bhaskaran, Rajith, Chandrasekaran, Gayathri, Kwon, Min-Chul, Bombardelli, Lorenzo, Monkhorst, Kim, Grasso, Cristoforo, Zevenhoven, John, van der Vliet, Jan, Cozijnsen, Miranda, Krimpenfort, Paul, Peeper, Daniel, van Lohuizen, Maarten, Berns, Anton
Format: Journal Article
Language:English
Published: United States 01.06.2020
Subjects:
Animals
Cyclin-Dependent Kinase Inhibitor p15 - metabolism
Cyclin-Dependent Kinase Inhibitor p16 - metabolism
Disease Models, Animal
Disease Progression
Gene Deletion
Humans
Immunophenotyping
MAP Kinase Signaling System - drug effects
Mesothelioma, Malignant - genetics
Mesothelioma, Malignant - metabolism
Mesothelioma, Malignant - pathology
Mice
Neurofibromin 2 - metabolism
Phosphatidylinositol 3-Kinases - metabolism
Poly(ADP-ribose) Polymerase Inhibitors - pharmacology
Protein Kinase Inhibitors - pharmacology
Transcription, Genetic - drug effects
Tumor Microenvironment - drug effects
Tumor Suppressor Proteins - metabolism
Ubiquitin Thiolesterase - metabolism
ISSN:1540-9538, 1540-9538
Online Access:Get more information
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Summary:We have generated mouse models of malignant mesothelioma (MM) based upon disruption of the Bap1, Nf2, and Cdkn2ab tumor suppressor loci in various combinations as also frequently observed in human MM. Inactivation of all three loci in the mesothelial lining of the thoracic cavity leads to a highly aggressive MM that recapitulates the histological features and gene expression profile observed in human patients. The tumors also show a similar inflammatory phenotype. Bap1 deletion alone does not cause MM but dramatically accelerates MM development when combined with Nf2 and Cdkn2ab (hereafter BNC) disruption. The accelerated tumor development is accompanied by increased Polycomb repression and EZH2-mediated redistribution of H3K27me3 toward promoter sites with concomitant activation of PI3K and MAPK pathways. Treatment of BNC tumor-bearing mice with cisplatin and pemetrexed, the current frontline treatment, prolongs survival. This makes the autochthonous mouse model described here very well suited to explore the pathogenesis of MM and validate new treatment regimens for MM, including immunotherapy.
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ISSN:1540-9538
1540-9538
DOI:10.1084/jem.20191257