Comparison of culture and polymerase chain reaction-restriction fragment length polymorphism for identification of various Capnocytophaga species from subgingival plaque samples of healthy and periodontally diseased individuals
Introduction: Capnocytophaga are facultative anaerobic Gram-negative bacilli and recognized as opportunistic pathogens of various extraoral infections. Only a few studies attempted to identify all the seven species of Capnocytophaga phenotypically and genotypically in healthy individuals and patient...
Uloženo v:
| Vydáno v: | Journal of oral and maxillofacial pathology : JOMFP Ročník 26; číslo 2; s. 287 |
|---|---|
| Hlavní autoři: | , , , , , |
| Médium: | Journal Article |
| Jazyk: | angličtina |
| Vydáno: |
Chennai
Wolters Kluwer India Pvt. Ltd
01.04.2022
Medknow Publications and Media Pvt. Ltd Medknow Publications & Media Pvt. Ltd Wolters Kluwer - Medknow |
| Témata: | |
| ISSN: | 0973-029X, 1998-393X |
| On-line přístup: | Získat plný text |
| Tagy: |
Přidat tag
Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!
|
| Abstract | Introduction: Capnocytophaga are facultative anaerobic Gram-negative bacilli and recognized as opportunistic pathogens of various extraoral infections. Only a few studies attempted to identify all the seven species of Capnocytophaga phenotypically and genotypically in healthy individuals and patients with chronic periodontitis. Studies to determine the prevalence of Capnocytophaga in subgingival plaque samples from healthy individuals, chronic gingivitis and periodontitis among Indian population are lacking.
Aim: The aim of this study was to identify and compare the presence of Capnocytophaga species phenotypically through microbial culture and biochemical tests and genotypically through polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in subgingival plaque of healthy individuals and patients with chronic gingivitis and chronic periodontitis.
Materials and Methods: A total of 300 subjects, 100 each with gingivitis, periodontitis and periodontally healthy gingiva subjected, were included. Subgingival plaque was collected and was cultured for phenotypic identification (microbial culture and biochemical test), and for genotypic identification, DNA extraction was done and PCR-RFLP analysis was performed to identify the genus Capnocytophaga and also to identify different species of Capnocytophaga.
Results: Of 300 individuals, Capnocytophaga species were identified from 237 (79%) individuals by PCR and 82 (27.33%) by culture. The prevalence of Capnocytophaga ochracea was found to be higher with both the methods followed by Capnocytophaga gingivalis and Capnocytophaga granulosa. Capnocytophaga genospecies, Capnocytophaga leadbetteri and Capnocytophaga Sputigena were isolated only by culture with very low prevalence that is 1.33%, 1.33% and 0.66%, respectively. We could not get any isolate of Capnocytophaga haemolytica by any of the two methods.
Conclusion: Capnocytophaga species could be found in gingival sulci as well as periodontal pockets and can be detected by culture and PCR-RFLP. However, higher prevalence of these species in healthy compared to disease requires further analysis to determine their role in healthy and diseased periodontium. |
|---|---|
| AbstractList | Introduction: Capnocytophaga are facultative anaerobic Gram-negative bacilli and recognized as opportunistic pathogens of various extraoral infections. Only a few studies attempted to identify all the seven species of Capnocytophaga phenotypically and genotypically in healthy individuals and patients with chronic periodontitis. Studies to determine the prevalence of Capnocytophaga in subgingival plaque samples from healthy individuals, chronic gingivitis and periodontitis among Indian population are lacking.
Aim: The aim of this study was to identify and compare the presence of Capnocytophaga species phenotypically through microbial culture and biochemical tests and genotypically through polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in subgingival plaque of healthy individuals and patients with chronic gingivitis and chronic periodontitis.
Materials and Methods: A total of 300 subjects, 100 each with gingivitis, periodontitis and periodontally healthy gingiva subjected, were included. Subgingival plaque was collected and was cultured for phenotypic identification (microbial culture and biochemical test), and for genotypic identification, DNA extraction was done and PCR-RFLP analysis was performed to identify the genus Capnocytophaga and also to identify different species of Capnocytophaga.
Results: Of 300 individuals, Capnocytophaga species were identified from 237 (79%) individuals by PCR and 82 (27.33%) by culture. The prevalence of Capnocytophaga ochracea was found to be higher with both the methods followed by Capnocytophaga gingivalis and Capnocytophaga granulosa. Capnocytophaga genospecies, Capnocytophaga leadbetteri and Capnocytophaga Sputigena were isolated only by culture with very low prevalence that is 1.33%, 1.33% and 0.66%, respectively. We could not get any isolate of Capnocytophaga haemolytica by any of the two methods.
Conclusion: Capnocytophaga species could be found in gingival sulci as well as periodontal pockets and can be detected by culture and PCR-RFLP. However, higher prevalence of these species in healthy compared to disease requires further analysis to determine their role in healthy and diseased periodontium. Capnocytophaga are facultative anaerobic Gram-negative bacilli and recognized as opportunistic pathogens of various extraoral infections. Only a few studies attempted to identify all the seven species of Capnocytophaga phenotypically and genotypically in healthy individuals and patients with chronic periodontitis. Studies to determine the prevalence of Capnocytophaga in subgingival plaque samples from healthy individuals, chronic gingivitis and periodontitis among Indian population are lacking.IntroductionCapnocytophaga are facultative anaerobic Gram-negative bacilli and recognized as opportunistic pathogens of various extraoral infections. Only a few studies attempted to identify all the seven species of Capnocytophaga phenotypically and genotypically in healthy individuals and patients with chronic periodontitis. Studies to determine the prevalence of Capnocytophaga in subgingival plaque samples from healthy individuals, chronic gingivitis and periodontitis among Indian population are lacking.The aim of this study was to identify and compare the presence of Capnocytophaga species phenotypically through microbial culture and biochemical tests and genotypically through polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in subgingival plaque of healthy individuals and patients with chronic gingivitis and chronic periodontitis.AimThe aim of this study was to identify and compare the presence of Capnocytophaga species phenotypically through microbial culture and biochemical tests and genotypically through polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in subgingival plaque of healthy individuals and patients with chronic gingivitis and chronic periodontitis.A total of 300 subjects, 100 each with gingivitis, periodontitis and periodontally healthy gingiva subjected, were included. Subgingival plaque was collected and was cultured for phenotypic identification (microbial culture and biochemical test), and for genotypic identification, DNA extraction was done and PCR-RFLP analysis was performed to identify the genus Capnocytophaga and also to identify different species of Capnocytophaga.Materials and MethodsA total of 300 subjects, 100 each with gingivitis, periodontitis and periodontally healthy gingiva subjected, were included. Subgingival plaque was collected and was cultured for phenotypic identification (microbial culture and biochemical test), and for genotypic identification, DNA extraction was done and PCR-RFLP analysis was performed to identify the genus Capnocytophaga and also to identify different species of Capnocytophaga.Of 300 individuals, Capnocytophaga species were identified from 237 (79%) individuals by PCR and 82 (27.33%) by culture. The prevalence of Capnocytophaga ochracea was found to be higher with both the methods followed by Capnocytophaga gingivalis and Capnocytophaga granulosa. Capnocytophaga genospecies, Capnocytophaga leadbetteri and Capnocytophaga Sputigena were isolated only by culture with very low prevalence that is 1.33%, 1.33% and 0.66%, respectively. We could not get any isolate of Capnocytophaga haemolytica by any of the two methods.ResultsOf 300 individuals, Capnocytophaga species were identified from 237 (79%) individuals by PCR and 82 (27.33%) by culture. The prevalence of Capnocytophaga ochracea was found to be higher with both the methods followed by Capnocytophaga gingivalis and Capnocytophaga granulosa. Capnocytophaga genospecies, Capnocytophaga leadbetteri and Capnocytophaga Sputigena were isolated only by culture with very low prevalence that is 1.33%, 1.33% and 0.66%, respectively. We could not get any isolate of Capnocytophaga haemolytica by any of the two methods.Capnocytophaga species could be found in gingival sulci as well as periodontal pockets and can be detected by culture and PCR-RFLP. However, higher prevalence of these species in healthy compared to disease requires further analysis to determine their role in healthy and diseased periodontium.ConclusionCapnocytophaga species could be found in gingival sulci as well as periodontal pockets and can be detected by culture and PCR-RFLP. However, higher prevalence of these species in healthy compared to disease requires further analysis to determine their role in healthy and diseased periodontium. Introduction: Capnocytophaga are facultative anaerobic Gram-negative bacilli and recognized as opportunistic pathogens of various extraoral infections. Only a few studies attempted to identify all the seven species of Capnocytophaga phenotypically and genotypically in healthy individuals and patients with chronic periodontitis. Studies to determine the prevalence of Capnocytophaga in subgingival plaque samples from healthy individuals, chronic gingivitis and periodontitis among Indian population are lacking. Aim: The aim of this study was to identify and compare the presence of Capnocytophaga species phenotypically through microbial culture and biochemical tests and genotypically through polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) in subgingival plaque of healthy individuals and patients with chronic gingivitis and chronic periodontitis. Materials and Methods: A total of 300 subjects, 100 each with gingivitis, periodontitis and periodontally healthy gingiva subjected, were included. Subgingival plaque was collected and was cultured for phenotypic identification (microbial culture and biochemical test), and for genotypic identification, DNA extraction was done and PCR-RFLP analysis was performed to identify the genus Capnocytophaga and also to identify different species of Capnocytophaga. Results: Of 300 individuals, Capnocytophaga species were identified from 237 (79%) individuals by PCR and 82 (27.33%) by culture. The prevalence of Capnocytophaga ochracea was found to be higher with both the methods followed by Capnocytophaga gingivalis and Capnocytophaga granulosa. Capnocytophaga genospecies, Capnocytophaga leadbetteri and Capnocytophaga Sputigena were isolated only by culture with very low prevalence that is 1.33%, 1.33% and 0.66%, respectively. We could not get any isolate of Capnocytophaga haemolytica by any of the two methods. Conclusion: Capnocytophaga species could be found in gingival sulci as well as periodontal pockets and can be detected by culture and PCR-RFLP. However, higher prevalence of these species in healthy compared to disease requires further analysis to determine their role in healthy and diseased periodontium. |
| Audience | Academic |
| Author | Kulkarni, R Bhat, Kishore Kotrashetti, Vijayalakshmi Kugaji, Manohar Idate, Ulka Kumbar, Vijay |
| AuthorAffiliation | 3 Department of Oral Pathology and Microbiology, Maratha Mandal's NGH Institute of Dental Sciences and Research Centre, Belgaum, Karnataka, India 2 Department of Microbiology, SDM Medical College, Dharwad, Karnataka, India 1 Department of Microbiology, Maratha Mandal's NGH Institute of Dental Sciences and Research Centre, Belgaum, Karnataka, India |
| AuthorAffiliation_xml | – name: 1 Department of Microbiology, Maratha Mandal's NGH Institute of Dental Sciences and Research Centre, Belgaum, Karnataka, India – name: 2 Department of Microbiology, SDM Medical College, Dharwad, Karnataka, India – name: 3 Department of Oral Pathology and Microbiology, Maratha Mandal's NGH Institute of Dental Sciences and Research Centre, Belgaum, Karnataka, India |
| Author_xml | – sequence: 1 givenname: Ulka surname: Idate fullname: Idate, Ulka organization: Department of Microbiology, Maratha Mandal's NGH Institute of Dental Sciences and Research Centre, Belgaum, Karnataka – sequence: 2 givenname: Kishore surname: Bhat fullname: Bhat, Kishore organization: Department of Microbiology, Maratha Mandal's NGH Institute of Dental Sciences and Research Centre, Belgaum, Karnataka – sequence: 3 givenname: R surname: Kulkarni fullname: Kulkarni, R organization: Department of Microbiology, SDM Medical College, Dharwad, Karnataka – sequence: 4 givenname: Vijayalakshmi surname: Kotrashetti fullname: Kotrashetti, Vijayalakshmi organization: Department of Oral Pathology and Microbiology, Maratha Mandal's NGH Institute of Dental Sciences and Research Centre, Belgaum, Karnataka – sequence: 5 givenname: Manohar surname: Kugaji fullname: Kugaji, Manohar organization: Department of Microbiology, Maratha Mandal's NGH Institute of Dental Sciences and Research Centre, Belgaum, Karnataka – sequence: 6 givenname: Vijay surname: Kumbar fullname: Kumbar, Vijay organization: Department of Microbiology, Maratha Mandal's NGH Institute of Dental Sciences and Research Centre, Belgaum, Karnataka |
| BookMark | eNp9Ustu3CAUtapUzSTtD3SF1E03nmJjG7OpFE36kiJ100rZIQYuNhMMLtgzmu_tj5SZSdQmqiIkQNxzzn1wLrIz5x1k2dsCL6sCkw8bP-hxedx5QUteFi-yRcFYmxNGbs-yBWaU5Lhkt-fZRYwbjOu2qstX2TmpWdMWFC-y3ys_jCKY6B3yGsnZTnMAJJxCo7f7AYKIgGQvjEMBhJyMd3mAOAVzvCMdRDeAm5AF1039ieXD2Js4IO0DMipFjTZSHPEpyTbl83NEKzE6L_eTH3vRCRRHkAZiUvQDivO6M64zW2HRaMWvGVAUw2hTPCn0IOzU709lQlJT3k3C2j1SJkKqWCHjlNkaNQsbX2cvdTrgzf15mf38_OnH6mt-8_3Lt9XVTS6rGsecMlaVTGNMNSs1VrWWlRRMEdxqoDWhDDeqXUu6bjCQVhWKFXqtKSgqicKMXGYfT7rjvB5AydR3EJaPwQwi7LkXhj-OONPzzm85I03V1CQJvL8XCD51HCc-mCjBWuEgDYyXFJdVW-O2TtB3T6AbPweX2uNl01JGGK3xX1QnLHDjtE955UGUX9GqTl6hzSHt8j-otBQMRibPaZPeHxHaE0EGH2MAzaWZjt-biMbyAvODQfnJmv8aNFHLJ9SH8TxLuj6Rdt5OEOKdnXcQeJrknfO7Z5i8bCl_cDj5A50yEDw |
| CitedBy_id | crossref_primary_10_3389_fmicb_2024_1296753 crossref_primary_10_3390_biology14080980 crossref_primary_10_3892_br_2024_1787 |
| Cites_doi | 10.1111/j.1600-051X.1988.tb01590.x 10.1111/j.1600-0765.1985.tb00830.x 10.1099/ijs.0.65373-0 10.1128/JCM.00169-10 10.1034/j.1600-0765.2000.035001033.x 10.1902/jop.2000.71.2.249 10.15713/ins.jcri.238 10.1111/j.1399-302X.1987.tb00293.x 10.1177/154405910308200503 10.1128/JCM.43.4.1894-1901.2005 10.1016/j.jmii.2016.04.007 10.1016/j.anaerobe.2010.02.005 10.5504/BBEQ.2013.0027 10.1177/2229411217729105 10.1902/jop.2002.73.1.20 10.1111/j.1348-0421.2001.tb01269.x 10.1111/j.1365-2672.1995.tb03424.x 10.1111/j.1399-302X.1990.tb00225.x 10.1016/j.ijantimicag.2006.10.005 10.1016/j.job.2014.08.001 10.1111/j.1600-0765.1984.tb00800.x 10.1099/00207713-44-2-324 10.1902/jop.1996.67.10.994 10.1902/jop.1983.54.7.420 10.1034/j.1600-051x.2001.028007701.x 10.4103/jomfp.JOMFP_33_20 |
| ContentType | Journal Article |
| Copyright | COPYRIGHT 2022 Medknow Publications and Media Pvt. Ltd. 2022. This article is published under (http://creativecommons.org/licenses/by-nc-sa/3.0/) (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. Copyright: © 2022 Journal of Oral and Maxillofacial Pathology. Copyright: © 2022 Journal of Oral and Maxillofacial Pathology 2022 |
| Copyright_xml | – notice: COPYRIGHT 2022 Medknow Publications and Media Pvt. Ltd. – notice: 2022. This article is published under (http://creativecommons.org/licenses/by-nc-sa/3.0/) (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. – notice: Copyright: © 2022 Journal of Oral and Maxillofacial Pathology. – notice: Copyright: © 2022 Journal of Oral and Maxillofacial Pathology 2022 |
| DBID | AAYXX CITATION 3V. 7X7 7XB 8FE 8FH 8FI 8FJ 8FK 8G5 ABUWG AFKRA AZQEC BBNVY BENPR BHPHI CCPQU DWQXO FYUFA GHDGH GNUQQ GUQSH HCIFZ K9. LK8 M0S M2O M7P MBDVC PHGZM PHGZT PIMPY PJZUB PKEHL PPXIY PQEST PQGLB PQQKQ PQUKI PRINS Q9U 7X8 5PM |
| DOI | 10.4103/jomfp.jomfp_172_21 |
| DatabaseName | CrossRef ProQuest Central (Corporate) Health & Medical Collection ProQuest Central (purchase pre-March 2016) ProQuest SciTech Collection ProQuest Natural Science Collection ProQuest Hospital Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) Research Library (Alumni) ProQuest Central (Alumni) ProQuest Central UK/Ireland ProQuest Central Essentials - QC ProQuest : Biological Science Collection journals [unlimited simultaneous users] ProQuest Central Natural Science Collection ProQuest One Community College ProQuest Central Health Research Premium Collection Health Research Premium Collection (Alumni) ProQuest Central Student ProQuest Research Library SciTech Premium Collection ProQuest Health & Medical Complete (Alumni) Biological Sciences ProQuest Health & Medical Collection Research Library Biological Science Database Research Library (Corporate) ProQuest Central Premium ProQuest One Academic ProQuest Publicly Available Content ProQuest Health & Medical Research Collection ProQuest One Academic Middle East (New) ProQuest One Health & Nursing ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Applied & Life Sciences ProQuest One Academic (retired) ProQuest One Academic UKI Edition ProQuest Central China ProQuest Central Basic MEDLINE - Academic PubMed Central (Full Participant titles) |
| DatabaseTitle | CrossRef Publicly Available Content Database Research Library Prep ProQuest Central Student ProQuest One Academic Middle East (New) ProQuest Central Essentials ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) SciTech Premium Collection ProQuest One Community College Research Library (Alumni Edition) ProQuest One Health & Nursing ProQuest Natural Science Collection ProQuest Central China ProQuest Central ProQuest One Applied & Life Sciences ProQuest Health & Medical Research Collection Health Research Premium Collection Health and Medicine Complete (Alumni Edition) Natural Science Collection ProQuest Central Korea Biological Science Collection ProQuest Research Library ProQuest Central (New) ProQuest Biological Science Collection ProQuest Central Basic ProQuest One Academic Eastern Edition ProQuest Hospital Collection Health Research Premium Collection (Alumni) Biological Science Database ProQuest SciTech Collection ProQuest Hospital Collection (Alumni) ProQuest Health & Medical Complete ProQuest One Academic UKI Edition ProQuest One Academic ProQuest One Academic (New) ProQuest Central (Alumni) MEDLINE - Academic |
| DatabaseTitleList | MEDLINE - Academic Publicly Available Content Database |
| Database_xml | – sequence: 1 dbid: PIMPY name: Publicly Available Content Database url: http://search.proquest.com/publiccontent sourceTypes: Aggregation Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Medicine |
| EISSN | 1998-393X |
| EndPage | 287 |
| ExternalDocumentID | PMC9364653 A745199763 10_4103_jomfp_jomfp_172_21 10.4103/jomfp.jomfp_172_21_287_Comparis |
| GeographicLocations | India |
| GeographicLocations_xml | – name: India |
| GroupedDBID | 29L 2WC 34H 53G 5GY 5VS 7X7 8FE 8FH 8FI 8FJ 8G5 ABJNI ABUWG ACGFS ACPRK ADBBV ADJBI AFKRA AHMBA ALMA_UNASSIGNED_HOLDINGS AOIJS AZQEC BAWUL BBNVY BENPR BHPHI BPHCQ BVXVI CCPQU DIK DWQXO E3Z EOJEC F5P FYUFA GNUQQ GUQSH GX1 H13 HCIFZ HMCUK HYE IAO IHR ITC KQ8 LK8 M2O M48 M7P O5R O5S OBODZ OK1 OVD P2P PGMZT PHGZM PHGZT PIMPY PQQKQ PROAC RMW RNS RPM TEORI TR2 UKHRP W3E AAYXX ADRAZ AFFHD CITATION IHE IL9 IPNFZ OVT PQGLB RIG 3V. 7XB 8FK K9. MBDVC PJZUB PKEHL PPXIY PQEST PQUKI PRINS Q9U 7X8 5PM |
| ID | FETCH-LOGICAL-c450s-799429f007f92f0d5fc4ca9d308fe7537906d8bc7b60e38d1d91fbf7ed7c3d093 |
| IEDL.DBID | W3E |
| ISSN | 0973-029X |
| IngestDate | Tue Nov 04 02:05:31 EST 2025 Sun Nov 09 13:20:21 EST 2025 Sat Nov 29 15:00:13 EST 2025 Tue Nov 11 10:29:29 EST 2025 Tue Nov 04 17:53:38 EST 2025 Sat Nov 29 03:53:03 EST 2025 Tue Nov 18 22:44:09 EST 2025 Tue Jun 17 22:46:38 EDT 2025 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 2 |
| Keywords | phenotypic tests gingivitis polymerase chain reaction-restriction fragment length polymorphism Capnocytophaga chronic periodontitis |
| Language | English |
| License | http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c450s-799429f007f92f0d5fc4ca9d308fe7537906d8bc7b60e38d1d91fbf7ed7c3d093 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 |
| OpenAccessLink | http://www.jomfp.in/article.asp?issn=0973-029X;year=2022;volume=26;issue=2;spage=287;epage=287;aulast=;type=0 |
| PMID | 35968170 |
| PQID | 2687939750 |
| PQPubID | 226475 |
| PageCount | 1 |
| ParticipantIDs | pubmedcentral_primary_oai_pubmedcentral_nih_gov_9364653 proquest_miscellaneous_2702485085 proquest_journals_2687939750 gale_infotracmisc_A745199763 gale_infotracacademiconefile_A745199763 crossref_citationtrail_10_4103_jomfp_jomfp_172_21 crossref_primary_10_4103_jomfp_jomfp_172_21 wolterskluwer_medknow_10_4103_jomfp_jomfp_172_21_287_Comparis |
| PublicationCentury | 2000 |
| PublicationDate | 20220401 |
| PublicationDateYYYYMMDD | 2022-04-01 |
| PublicationDate_xml | – month: 4 year: 2022 text: 20220401 day: 01 |
| PublicationDecade | 2020 |
| PublicationPlace | Chennai |
| PublicationPlace_xml | – name: Chennai – name: India |
| PublicationTitle | Journal of oral and maxillofacial pathology : JOMFP |
| PublicationYear | 2022 |
| Publisher | Wolters Kluwer India Pvt. Ltd Medknow Publications and Media Pvt. Ltd Medknow Publications & Media Pvt. Ltd Wolters Kluwer - Medknow |
| Publisher_xml | – name: Wolters Kluwer India Pvt. Ltd – name: Medknow Publications and Media Pvt. Ltd – name: Medknow Publications & Media Pvt. Ltd – name: Wolters Kluwer - Medknow |
| References | Socransky (R15-30-20240906) 2010; 16 Kugaji (R14-30-20240906) 2017; 8 Tanner (R4-30-20240906) 2015; 57 Jolivet-Gougeon (R6-30-20240906) 2007; 29 Ciantar (R8-30-20240906) 2001; 28 Frisken (R18-30-20240906) 1990; 5 Savitt (R19-30-20240906) 1984; 19 Amano (R24-30-20240906) 2000; 71 Popova (R1-30-20240906) 2013; 27 Tsai (R2-30-20240906) 2018; 51 Kesic (R3-30-20240906) 2008; 15 Ciantar (R10-30-20240906) 2005; 43 Kimura (R21-30-20240906) 2002; 73 Cloud (R9-30-20240906) 2010; 48 Mashimo (R28-30-20240906) 1983; 54 Frandsen (R7-30-20240906) 2008; 58 Idate (R11-30-20240906) 2018; 5 Dzink (R29-30-20240906) 1988; 15 Conrads (R25-30-20240906) 1996; 67 Delaney (R26-30-20240906) 1987; 2 Wilson (R23-30-20240906) 1995; 78 Kamma (R22-30-20240906) 2000; 35 Kumar (R27-30-20240906) 2003; 82 Hayashi (R17-30-20240906) 2001; 45 Holdman (R20-30-20240906) 1985; 20 Yamamoto (R13-30-20240906) 1994; 44 Idate (R12-30-20240906) 2020; 24 Urbán (R16-30-20240906) 2010; 16 |
| References_xml | – volume: 15 start-page: 316 year: 1988 ident: R29-30-20240906 article-title: The predominant cultivable microbiota of active and inactive lesions of destructive periodontal diseases publication-title: J Clin Periodontol doi: 10.1111/j.1600-051X.1988.tb01590.x – volume: 20 start-page: 475 year: 1985 ident: R20-30-20240906 article-title: Distribution of Capnocytophaga in periodontal microflora publication-title: J Periodontol Res doi: 10.1111/j.1600-0765.1985.tb00830.x – volume: 16 start-page: 283 year: 2010 ident: R15-30-20240906 article-title: The microbiota of the gingival crevice area of man.I publication-title: Total microscopic and viable counts and counts of specific organisms – volume: 58 start-page: 324 year: 2008 ident: R7-30-20240906 article-title: Diversity of Capnocytophaga species in children and description of Capnocytophaga leadbetteri sp. nov. and Capnocytophaga genospecies AHN8471 publication-title: Int J Syst Evol Microbiol doi: 10.1099/ijs.0.65373-0 – volume: 48 start-page: 1442 year: 2010 ident: R9-30-20240906 article-title: Comparison of traditional phenotypic identification methods with partial 5′ 16S rRNA gene sequencing for species-level identification of nonfermenting Gram-negative bacilli publication-title: J Clin Microbiol doi: 10.1128/JCM.00169-10 – volume: 35 start-page: 33 year: 2000 ident: R22-30-20240906 article-title: Profile of subgingival microbiota in children with primary dentition publication-title: J Periodontal Res doi: 10.1034/j.1600-0765.2000.035001033.x – volume: 71 start-page: 249 year: 2000 ident: R24-30-20240906 article-title: Periodontopathic bacteria in children with Down syndrome publication-title: J Periodontol doi: 10.1902/jop.2000.71.2.249 – volume: 5 start-page: 173 year: 2018 ident: R11-30-20240906 article-title: Identification of Capnocytophaga species from oral cavity of healthy individuals and patients with chronic periodontitis using phenotypic tests publication-title: J Adv Clin Res Insights doi: 10.15713/ins.jcri.238 – volume: 2 start-page: 71 year: 1987 ident: R26-30-20240906 article-title: Microbiology of subgingival plaque from children with localized prepubertal periodontitis publication-title: Oral Microbiol Immunol doi: 10.1111/j.1399-302X.1987.tb00293.x – volume: 82 start-page: 338 year: 2003 ident: R27-30-20240906 article-title: New bacterial species associated with chronic periodontitis publication-title: J Dent Res doi: 10.1177/154405910308200503 – volume: 43 start-page: 1894 year: 2005 ident: R10-30-20240906 article-title: Molecular identification of Capnocytophaga spp.via 16S rRNA PCR-restriction fragment length polymorphism analysis publication-title: J Clin Microbiol doi: 10.1128/JCM.43.4.1894-1901.2005 – volume: 51 start-page: 226 year: 2018 ident: R2-30-20240906 article-title: Subgingival microbiota in individuals with severe chronic periodontitis publication-title: J Microbiol Immunol Infect doi: 10.1016/j.jmii.2016.04.007 – volume: 16 start-page: 283 year: 2010 ident: R16-30-20240906 article-title: Detection of periodontopathogenic bacteria in pregnant women by traditional anaerobic culture method and by a commercial molecular genetic method publication-title: Anaerobe doi: 10.1016/j.anaerobe.2010.02.005 – volume: 27 start-page: 3754 year: 2013 ident: R1-30-20240906 article-title: Microbiology of periodontal diseases.A review publication-title: Biotechnol Biotechnol Equip doi: 10.5504/BBEQ.2013.0027 – volume: 8 start-page: 4752 year: 2017 ident: R14-30-20240906 article-title: Simplified method of detection of Dialister invisus and Olsenella uli in oral cavity samples by polymerase chain reaction publication-title: J Adv Oral Res doi: 10.1177/2229411217729105 – volume: 73 start-page: 20 year: 2002 ident: R21-30-20240906 article-title: Periodontopathic bacterial infection in childhood publication-title: J Periodontol doi: 10.1902/jop.2002.73.1.20 – volume: 45 start-page: 17 year: 2001 ident: R17-30-20240906 article-title: PCR detection of Capnocytophaga species in dental plaque samples from children aged 2 to 12 years publication-title: Microbiol Immunol doi: 10.1111/j.1348-0421.2001.tb01269.x – volume: 78 start-page: 394 year: 1995 ident: R23-30-20240906 article-title: Restriction fragment length polymorphism analysis of PCR-amplified 16S ribosomal DNA of human Capnocytophaga publication-title: J Appl Bacteriol doi: 10.1111/j.1365-2672.1995.tb03424.x – volume: 5 start-page: 43 year: 1990 ident: R18-30-20240906 article-title: The incidence of periodontopathic microorganisms in young children publication-title: Oral Microbiol Immunol doi: 10.1111/j.1399-302X.1990.tb00225.x – volume: 29 start-page: 367 year: 2007 ident: R6-30-20240906 article-title: Antimicrobial treatment of Capnocytophaga infections publication-title: Int J Antimicrob Agents doi: 10.1016/j.ijantimicag.2006.10.005 – volume: 57 start-page: 18 year: 2015 ident: R4-30-20240906 article-title: Anaerobic culture to detect periodontal and caries pathogens publication-title: J Oral Biosci doi: 10.1016/j.job.2014.08.001 – volume: 19 start-page: 111 year: 1984 ident: R19-30-20240906 article-title: Distribution of certain subgingival microbial species in selected periodontal conditions publication-title: J Periodontal Res doi: 10.1111/j.1600-0765.1984.tb00800.x – volume: 44 start-page: 324 year: 1994 ident: R13-30-20240906 article-title: Capnocytophaga haemolytica sp. nov. and Capnocytophaga granulosa sp. nov., from human dental plaque publication-title: Int J Syst Bacteriol doi: 10.1099/00207713-44-2-324 – volume: 67 start-page: 994 year: 1996 ident: R25-30-20240906 article-title: PCR reaction and dot-blot hybridization to monitor the distribution of oral pathogens within plaque samples of periodontally healthy individuals publication-title: J Periodontol doi: 10.1902/jop.1996.67.10.994 – volume: 15 start-page: 1 year: 2008 ident: R3-30-20240906 article-title: Microbial etiology of periodontal disease- mini review publication-title: Med Biol – volume: 54 start-page: 420 year: 1983 ident: R28-30-20240906 article-title: The periodontal microflora of juvenile diabetics.Culture, immunofluorescence, and serum antibody studies publication-title: J Periodontol doi: 10.1902/jop.1983.54.7.420 – volume: 28 start-page: 701 year: 2001 ident: R8-30-20240906 article-title: Capnocytophaga granulosa and Capnocytophaga haemolytica: Novel species in subgingival plaque publication-title: J Clin Periodontol doi: 10.1034/j.1600-051x.2001.028007701.x – volume: 24 start-page: 397 year: 2020 ident: R12-30-20240906 article-title: Molecular identification of Capnocytophaga species from the oral cavity of patients with chronic periodontitis and healthy individuals publication-title: J Oral Maxillofac Pathol doi: 10.4103/jomfp.JOMFP_33_20 |
| SSID | ssj0058452 |
| Score | 2.239744 |
| Snippet | Introduction: Capnocytophaga are facultative anaerobic Gram-negative bacilli and recognized as opportunistic pathogens of various extraoral infections. Only a... Capnocytophaga are facultative anaerobic Gram-negative bacilli and recognized as opportunistic pathogens of various extraoral infections. Only a few studies... |
| SourceID | pubmedcentral proquest gale crossref wolterskluwer |
| SourceType | Open Access Repository Aggregation Database Enrichment Source Index Database Publisher |
| StartPage | 287 |
| SubjectTerms | Bacilli Capnocytophaga Comparative analysis Ethylenediaminetetraacetic acid Genetic aspects Gingiva Gingivitis Gram-negative bacilli Gum disease Medical research Medicine, Experimental Opportunist infection Original Periodontitis Periodontium Polymerase chain reaction Polymorphism Restriction fragment length polymorphism Species |
| SummonAdditionalLinks | – databaseName: Biological Science Database dbid: M7P link: http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1bi9QwFA66igjiXXZ0lQiCD1K3bdqmeRBZBhdfXPZBYd5Cm8tO15m2O5lZmTf_g_9Q8H94TpsZtwrz4svAkF4S-uWck-R83yHkVaQYS01sApHoNEgixYNScR6EQpWxgAg2TvtiE_zkJJ9MxKnfcHM-rXJjEztDrRuFe-SHcZYDlAQ4uPftRYBVo_B01ZfQuE5uoEoC61L3TjeWGHxrV3EHFWmCMBaTnjSTRCE7PG_mtn3b_Urw4TKOBo7pb_P8b8rknW8NHme7r102-xWfdHzvf0dzn9z10Sg96uHzgFwz9UNy65M_b39Efo23ZQppY2kv02FoUWvaNrM1bmg5Q9W0qGoK0WfHkfj5_QfW-1hU3T9qF8UZ7kBSLNmynPb3NfB1KzenEDHTSvuEpQ4j-JpLeGOzcnRctHWj1ih9UJwVFEmhsK6nyIihblVieaUKJgptZwWMnboClY4dPqFnd677jsIcg7U3sj5na-oPpDSttkw095h8Of7wefwx8IUhApWkoQu4EOBGLYQ3VsQ21KlViSqEZmFuDay_uAgznQPmyiw0LNeRFpEtLTeaK6ZDwZ6QvbqpzT6hJmGl1llqilwkVtgSmrnQmeVxmVjLRiTaoEIqr5qOxTtmElZPiCTZY-gqkkbkzfaettcM2Xn1awSbRIMCT1aF50VA_1CaSx5xVACCqBH6cjC4EgyBGjZvsCa9IXLyD9BG5OW2Ge_E5LrawNeUSElMcojU0xHhA5hvu48i5MOWupp2YuSCZSjRNyLvBhNCzns2546BS1isyw2Mn-7u_DNyO0beSZcydUD2louVeU5uqstl5RYvuln_GzbNcR4 priority: 102 providerName: ProQuest |
| Title | Comparison of culture and polymerase chain reaction-restriction fragment length polymorphism for identification of various Capnocytophaga species from subgingival plaque samples of healthy and periodontally diseased individuals |
| URI | http://www.jomfp.in/article.asp?issn=0973-029X;year=2022;volume=26;issue=2;spage=287;epage=287;aulast=;type=0 https://www.proquest.com/docview/2687939750 https://www.proquest.com/docview/2702485085 https://pubmed.ncbi.nlm.nih.gov/PMC9364653 |
| Volume | 26 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| journalDatabaseRights | – providerCode: PRVPQU databaseName: Biological Science Database customDbUrl: eissn: 1998-393X dateEnd: 20231031 omitProxy: false ssIdentifier: ssj0058452 issn: 0973-029X databaseCode: M7P dateStart: 20080101 isFulltext: true titleUrlDefault: http://search.proquest.com/biologicalscijournals providerName: ProQuest – providerCode: PRVPQU databaseName: Health & Medical Collection customDbUrl: eissn: 1998-393X dateEnd: 20231031 omitProxy: false ssIdentifier: ssj0058452 issn: 0973-029X databaseCode: 7X7 dateStart: 20080101 isFulltext: true titleUrlDefault: https://search.proquest.com/healthcomplete providerName: ProQuest – providerCode: PRVPQU databaseName: ProQuest Central customDbUrl: eissn: 1998-393X dateEnd: 20231031 omitProxy: false ssIdentifier: ssj0058452 issn: 0973-029X databaseCode: BENPR dateStart: 20080101 isFulltext: true titleUrlDefault: https://www.proquest.com/central providerName: ProQuest – providerCode: PRVPQU databaseName: ProQuest Research Library customDbUrl: eissn: 1998-393X dateEnd: 20231031 omitProxy: false ssIdentifier: ssj0058452 issn: 0973-029X databaseCode: M2O dateStart: 20080101 isFulltext: true titleUrlDefault: https://search.proquest.com/pqrl providerName: ProQuest – providerCode: PRVPQU databaseName: Publicly Available Content Database customDbUrl: eissn: 1998-393X dateEnd: 20231031 omitProxy: false ssIdentifier: ssj0058452 issn: 0973-029X databaseCode: PIMPY dateStart: 20080101 isFulltext: true titleUrlDefault: http://search.proquest.com/publiccontent providerName: ProQuest – providerCode: PRVEMX databaseName: Medknow : Open Access Journals [open access] customDbUrl: eissn: 1998-393X dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0058452 issn: 0973-029X databaseCode: W3E dateStart: 20030101 isFulltext: true titleUrlDefault: http://www.medknow.com/journals.asp providerName: Wolters Kluwer Health |
| link | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwvV3di9NAEF_OOxFB_Bar57GC4IOESzYfm6Xcw1l66ENrEYX6tCT7cY22SWnak_69_iPOZNNwVbgXwZeQZrPZSTozO7M7vxlC3gQqDGPDjCciHXtRoLiXK849X6icCbBgWeyKTfDxOJ1OxeSA7LZiMKrye7WwSzA7T9tv1yC62jUrjpuOYtrfAhuAN8xYvxVilvQdoazfSmLK-6Y7yzZgi67P-k0mIvDwjxiWpkMASjjcKW6YipsCPd0oDmMTBX546mhqjhKmfMmCvXnsT23-d4TlvZ8V7n7XP5rg92tT2MWD__zyD8n91tal526MR-TAlI_JnVG7m_-E_Bp0RRBpZalLAmJoVmq6rOZbXC6rDVWzrCgp2LYNAsPDWiKrojmndpVd4uomxXIw65nrVQHnFPWCgjVOC90GQzX8h4NcwXjVpqaDbFlWaotpFbLLjCLgtDA1RbQNrTc5lm4qQAjpcp7Bt6Z1hlmUa3yCQ45uHZkgv-DXI6J0vqXtZpemRYdyq5-SrxfDL4MPXlt0wlNR7NceFwKmaAumkxXM-jq2KlKZ0KGfWgO-HRd-olPg5zzxTZjqQIvA5pYbzVWofRE-I4dlVZrnhJoozLVOYpOlIrLC5tDMhU4sZ3lkbdgjwY6FpGozsmNhkLkEzwzZTjqGu852PfKu67N0-UhuvPstcqZEZQVPVlmLuQD6MO2XPOeYXQgsUqDleO9OUDJqv3nH27JVcrVkSQraXYDN2SOvu2bsiYF7pYF_UyLcMUrBC4h7hO_JREc-JjjfbymLWZPoXIQJpv_rkbM96ZELhxS94cUl8L3cMfGLf-z_ktxFaXPxWsfkcL3amFfktrpaF_XqhNziU94c0xNy9H44nnyGXyP2CY98AtcmH0eTbyeNrvkN6aCgAA |
| linkProvider | Wolters Kluwer Health |
| linkToHtml | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1V1Lb9QwELaqFgES4o1YKGAkEAcUmrfjQ4WqQtVVH9pDkcrJJH50A7tJWPehvfEf-B_8KCT-BzN5LA1IvfXAZaWV42TifJ4Z2_PNEPLCk0EQaV87PFSRE3qSOZlkzHG5zHwOHqwfNcUm2P5-cnjIR0vkR8eFwbDKTifWilqVEvfI1_w4AShxMHBvq68OVo3C09WuhEYDix09P4Mlm10fvoPv-9L3t94fbG47bVUBR4aRax3GOehgA7bRcN-4KjIylClXgZsYDc47426sEhA4i10dJMpT3DOZYVoxGag6-RKo_JUQwJ4sk5XRcG_0sdP9YM3rGj-YA8dxfX7Y0HRCzw3WPpdTU72pfwV4DcL3eqbwb4Pwb5DmjbMSD9Dtlzp-_pwV3Lr1v43fbXKz9bfpRjNB7pAlXdwlV_faiIJ75NfmohAjLQ1tEpFomhaKVuVkjlt2VlM5TvOCgn9ds0B-fvuOFU1mef2Pmll6hHusFIvSHI-bfiXgN7dTCmsCmqs2JKueBfiYU3hieWLpZloVpZxjcof0KKVIe821pcj5ofYkwwJSOagCWk1SGGtqU8zlbPEODX913ggKWqRUJfJaJ3PaHrkpmi-4dvY--XApg_yALBdloR8SqsMgUyqOdJrw0HCTQTPjKjbMz0JjggHxOhQK2eaFx_IkEwHrQ0SuaDB7HrkD8nrRp2qyolx49SsEt0CVCXeWacv8APkw-ZjYYJjjCPxikGW1dyWoOtlv7rAtWlVrxR9gD8jzRTP2xPDBQsPXFEi6DBNYi0QDwnrTaiE-plnvtxT5uE63zoMYkxAOyHpvAoppw1e94MWFnzDRwfjRxcI_I9e2D_Z2xe5wf-cxue4jy6YOEFsly8ezE_2EXJGnx7mdPW11DiWfLnua_gZModAs |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Comparison+of+culture+and+polymerase+chain+reaction%E2%80%93restriction+fragment+length+polymorphism+for+identification+of+various+Capnocytophaga+species+from+subgingival+plaque+samples+of+healthy+and+periodontally+diseased+individuals&rft.jtitle=Journal+of+oral+and+maxillofacial+pathology+%3A+JOMFP&rft.au=Idate%2C+Ulka&rft.au=Bhat%2C+Kishore&rft.au=Kulkarni%2C+R+D&rft.au=Kotrashetti%2C+Vijayalakshmi&rft.date=2022-04-01&rft.pub=Wolters+Kluwer+-+Medknow&rft.issn=0973-029X&rft.eissn=1998-393X&rft.volume=26&rft.issue=2&rft.spage=287&rft.epage=287&rft_id=info:doi/10.4103%2Fjomfp.jomfp_172_21&rft_id=info%3Apmid%2F35968170&rft.externalDocID=PMC9364653 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0973-029X&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0973-029X&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0973-029X&client=summon |