An accurate and sensitive Coomassie Brilliant Blue G-250-based assay for protein determination

In this protocol we present a rapid and sensitive assay for the accurate determination of protein concentration. The assay is a modification of a previous method, and measures minimum 0.2 μg protein.In this protocol we present a rapid and sensitive assay for the accurate determination of protein con...

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Veröffentlicht in:	Analytical biochemistry Jg. 480; S. 28 - 30
Hauptverfasser:	Grintzalis, Konstantinos, Georgiou, Christos D., Schneider, Yves-Jacques
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	United States Elsevier Inc 01.07.2015
Schlagworte:	bioassays Bradford Coomassie Brilliant Blue G (CBB) dyes Protein quantification proteins Proteins - analysis protocols rapid methods Rosaniline Dyes - chemistry Coomassie Brilliant Blue G (CBB) Bradford Protein quantification
ISSN:	0003-2697, 1096-0309, 1096-0309
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Abstract	In this protocol we present a rapid and sensitive assay for the accurate determination of protein concentration. The assay is a modification of a previous method, and measures minimum 0.2 μg protein.In this protocol we present a rapid and sensitive assay for the accurate determination of protein concentration. The assay is a modification of a previous method, and measures minimum 0.2 μg protein.
AbstractList	In this protocol we present a rapid and sensitive assay for the accurate determination of protein concentration. The assay is a modification of a previous method, and measures minimum 0.2 μg protein.In this protocol we present a rapid and sensitive assay for the accurate determination of protein concentration. The assay is a modification of a previous method, and measures minimum 0.2 μg protein. In this protocol we present a rapid and sensitive assay for the accurate determination of protein concentration. The assay is a modification of a previous method, and measures minimum 0.2 μg protein. In this protocol we present a rapid and sensitive assay for the accurate determination of protein concentration. The assay is a modification of a previous method, and measures minimum 0.2μg protein.
Author	Georgiou, Christos D. Grintzalis, Konstantinos Schneider, Yves-Jacques
Author_xml	– sequence: 1 givenname: Konstantinos surname: Grintzalis fullname: Grintzalis, Konstantinos email: kgrintzalis@gmail.com organization: Laboratory of Cellular, Nutritional and Toxicological Biochemistry, Institute of Life Sciences, Université Catholique de Louvain (UCL), Louvain-la-Neuve, Belgium – sequence: 2 givenname: Christos D. surname: Georgiou fullname: Georgiou, Christos D. organization: Department of Biology, Section of Genetics, Cell Biology and Development, University of Patras, Patras, Greece – sequence: 3 givenname: Yves-Jacques surname: Schneider fullname: Schneider, Yves-Jacques organization: Laboratory of Cellular, Nutritional and Toxicological Biochemistry, Institute of Life Sciences, Université Catholique de Louvain (UCL), Louvain-la-Neuve, Belgium
BackLink	https://www.ncbi.nlm.nih.gov/pubmed/25837770$$D View this record in MEDLINE/PubMed
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Keywords	Coomassie Brilliant Blue G (CBB) Bradford Protein quantification
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Snippet	In this protocol we present a rapid and sensitive assay for the accurate determination of protein concentration. The assay is a modification of a previous...
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SubjectTerms	bioassays Bradford Coomassie Brilliant Blue G (CBB) dyes Protein quantification proteins Proteins - analysis protocols rapid methods Rosaniline Dyes - chemistry
Title	An accurate and sensitive Coomassie Brilliant Blue G-250-based assay for protein determination
URI	https://dx.doi.org/10.1016/j.ab.2015.03.024 https://www.ncbi.nlm.nih.gov/pubmed/25837770 https://www.proquest.com/docview/1680958492 https://www.proquest.com/docview/2101347987
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