'Candidatus Phytoplasma sudamericanum', a novel taxon, and strain PassWB-Br4, a new subgroup 16SrIII-V phytoplasma, from diseased passion fruit (Passiflora edulis f. flavicarpa Deg.)

Symptoms of abnormal proliferation of shoots resulting in formation of witches'-broom growths were observed on diseased plants of passion fruit (Passiflora edulis f. flavicarpa Deg.) in Brazil. RFLP analysis of 16S rRNA gene sequences amplified in PCRs containing template DNAs extracted from di...

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Bibliographic Details
Published in:International journal of systematic and evolutionary microbiology Vol. 62; no. Pt 4; p. 984
Main Authors: Davis, Robert E, Zhao, Yan, Dally, Ellen L, Jomantiene, Rasa, Lee, Ing-Ming, Wei, Wei, Kitajima, Elliot W
Format: Journal Article
Language:English
Published: England 01.04.2012
Subjects:
Brazil
Molecular Sequence Data
Passiflora - microbiology
Phylogeny
Phytoplasma - classification
Phytoplasma - genetics
Phytoplasma - isolation & purification
Plant Diseases - microbiology
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
RNA, Ribosomal, 16S - genetics
Sequence Alignment
Brazil
ISSN:1466-5034, 1466-5034
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Summary:Symptoms of abnormal proliferation of shoots resulting in formation of witches'-broom growths were observed on diseased plants of passion fruit (Passiflora edulis f. flavicarpa Deg.) in Brazil. RFLP analysis of 16S rRNA gene sequences amplified in PCRs containing template DNAs extracted from diseased plants collected in Bonito (Pernambuco) and Viçosa (Minas Gerais) Brazil, indicated that such symptoms were associated with infections by two mutually distinct phytoplasmas. One phytoplasma, PassWB-Br4 from Bonito, represents a new subgroup, 16SrIII-V, in the X-disease phytoplasma group ('Candidatus Phytoplasma pruni'-related strains). The second phytoplasma, PassWB-Br3 from Viçosa, represents a previously undescribed subgroup in group 16SrVI. Phylogenetic analyses of 16S rRNA gene sequences were consistent with the hypothesis that strain PassWB-Br3 is distinct from previously described 'Ca. Phytoplasma' species. Nucleotide sequence alignments revealed that strain PassWB-Br3 shared less than 97.5 % 16S rRNA gene sequence similarity with previously described 'Ca. Phytoplasma' species. The unique properties of its DNA, in addition to natural host and geographical occurrence, support the recognition of strain PassWB-Br3 as a representative of a novel taxon, 'Candidatus Phytoplasma sudamericanum'.
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ISSN:1466-5034
1466-5034
DOI:10.1099/ijs.0.033423-0