Adipose‐derived stem cell spheroids are superior to single‐cell suspensions to improve fat autograft long‐term survival
Autologous fat transplantation is a widely used procedure for surgical reconstruction of tissues. The resorption rate of this transplantation remains high and unpredictable, reinforcing the need of adjuvant treatments that increase the long‐term stability of grafts. Adipose‐derived stem cells (ASC)...
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| Vydáno v: | Journal of cellular and molecular medicine Ročník 26; číslo 5; s. 1421 - 1433 |
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| Hlavní autoři: | , , , , , , , |
| Médium: | Journal Article |
| Jazyk: | angličtina |
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England
John Wiley & Sons, Inc
01.03.2022
John Wiley and Sons Inc |
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| ISSN: | 1582-1838, 1582-4934, 1582-4934 |
| On-line přístup: | Získat plný text |
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| Abstract | Autologous fat transplantation is a widely used procedure for surgical reconstruction of tissues. The resorption rate of this transplantation remains high and unpredictable, reinforcing the need of adjuvant treatments that increase the long‐term stability of grafts. Adipose‐derived stem cells (ASC) introduced as single cells in fat has been shown clinically to reduce the resorption of fat grafts. On the other hand, the formulation of ASC into cell spheroids results in the enhancement of their regenerative potential. In this study, we developed a novel method to produce highly homogeneous ASC spheroids and characterized their features and efficacy on fat transplantation. Spheroids conserved ASC markers and multipotency. A regenerative gene expression profile was maintained, and genes linked to autophagy were upregulated whereas proliferation was decreased. Their secreted proteome was enriched in comparison with single‐cell ASC suspension. Addition of spheroids to fat graft in an animal model of transplantation resulted in a better graft long‐term stability when compared to single ASC suspension. In conclusion, we provide a novel method to manufacture homogenous ASC spheroids. These ASC spheroids are superior to ASC in single‐cell suspension to improve the stability of fat transplants, reinforcing their potential in reconstructive surgery. |
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| AbstractList | Autologous fat transplantation is a widely used procedure for surgical reconstruction of tissues. The resorption rate of this transplantation remains high and unpredictable, reinforcing the need of adjuvant treatments that increase the long‐term stability of grafts. Adipose‐derived stem cells (ASC) introduced as single cells in fat has been shown clinically to reduce the resorption of fat grafts. On the other hand, the formulation of ASC into cell spheroids results in the enhancement of their regenerative potential. In this study, we developed a novel method to produce highly homogeneous ASC spheroids and characterized their features and efficacy on fat transplantation. Spheroids conserved ASC markers and multipotency. A regenerative gene expression profile was maintained, and genes linked to autophagy were upregulated whereas proliferation was decreased. Their secreted proteome was enriched in comparison with single‐cell ASC suspension. Addition of spheroids to fat graft in an animal model of transplantation resulted in a better graft long‐term stability when compared to single ASC suspension. In conclusion, we provide a novel method to manufacture homogenous ASC spheroids. These ASC spheroids are superior to ASC in single‐cell suspension to improve the stability of fat transplants, reinforcing their potential in reconstructive surgery. Autologous fat transplantation is a widely used procedure for surgical reconstruction of tissues. The resorption rate of this transplantation remains high and unpredictable, reinforcing the need of adjuvant treatments that increase the long-term stability of grafts. Adipose-derived stem cells (ASC) introduced as single cells in fat has been shown clinically to reduce the resorption of fat grafts. On the other hand, the formulation of ASC into cell spheroids results in the enhancement of their regenerative potential. In this study, we developed a novel method to produce highly homogeneous ASC spheroids and characterized their features and efficacy on fat transplantation. Spheroids conserved ASC markers and multipotency. A regenerative gene expression profile was maintained, and genes linked to autophagy were upregulated whereas proliferation was decreased. Their secreted proteome was enriched in comparison with single-cell ASC suspension. Addition of spheroids to fat graft in an animal model of transplantation resulted in a better graft long-term stability when compared to single ASC suspension. In conclusion, we provide a novel method to manufacture homogenous ASC spheroids. These ASC spheroids are superior to ASC in single-cell suspension to improve the stability of fat transplants, reinforcing their potential in reconstructive surgery.Autologous fat transplantation is a widely used procedure for surgical reconstruction of tissues. The resorption rate of this transplantation remains high and unpredictable, reinforcing the need of adjuvant treatments that increase the long-term stability of grafts. Adipose-derived stem cells (ASC) introduced as single cells in fat has been shown clinically to reduce the resorption of fat grafts. On the other hand, the formulation of ASC into cell spheroids results in the enhancement of their regenerative potential. In this study, we developed a novel method to produce highly homogeneous ASC spheroids and characterized their features and efficacy on fat transplantation. Spheroids conserved ASC markers and multipotency. A regenerative gene expression profile was maintained, and genes linked to autophagy were upregulated whereas proliferation was decreased. Their secreted proteome was enriched in comparison with single-cell ASC suspension. Addition of spheroids to fat graft in an animal model of transplantation resulted in a better graft long-term stability when compared to single ASC suspension. In conclusion, we provide a novel method to manufacture homogenous ASC spheroids. These ASC spheroids are superior to ASC in single-cell suspension to improve the stability of fat transplants, reinforcing their potential in reconstructive surgery. |
| Author | El Harane, Sanae Braschler, Thomas Brembilla, Nicolo Durual, Stéphane Krause, Karl‐Heinz Modarressi, Ali André‐Lévigne, Dominik Preynat‐Seauve, Olivier |
| AuthorAffiliation | 3 Division of Plastic, Reconstructive and Aesthetic Surgery Geneva University Hospitals University of Geneva Geneva Switzerland 2 Laboratory of Biomaterials Faculty of Dental Medicine University of Geneva Geneva Switzerland 1 Department of Pathology and Immunology Faculty of Medicine University of Geneva Geneva Switzerland 5 Department of Medicine Faculty of Medicine University of Geneva Geneva Switzerland 4 Laboratory of Therapy and Stem Cells Geneva University Hospitals Geneva Switzerland |
| AuthorAffiliation_xml | – name: 2 Laboratory of Biomaterials Faculty of Dental Medicine University of Geneva Geneva Switzerland – name: 1 Department of Pathology and Immunology Faculty of Medicine University of Geneva Geneva Switzerland – name: 4 Laboratory of Therapy and Stem Cells Geneva University Hospitals Geneva Switzerland – name: 5 Department of Medicine Faculty of Medicine University of Geneva Geneva Switzerland – name: 3 Division of Plastic, Reconstructive and Aesthetic Surgery Geneva University Hospitals University of Geneva Geneva Switzerland |
| Author_xml | – sequence: 1 givenname: Sanae surname: El Harane fullname: El Harane, Sanae organization: University of Geneva – sequence: 2 givenname: Stéphane surname: Durual fullname: Durual, Stéphane organization: University of Geneva – sequence: 3 givenname: Thomas surname: Braschler fullname: Braschler, Thomas organization: University of Geneva – sequence: 4 givenname: Dominik surname: André‐Lévigne fullname: André‐Lévigne, Dominik organization: University of Geneva – sequence: 5 givenname: Nicolo surname: Brembilla fullname: Brembilla, Nicolo organization: University of Geneva – sequence: 6 givenname: Karl‐Heinz surname: Krause fullname: Krause, Karl‐Heinz organization: Geneva University Hospitals – sequence: 7 givenname: Ali surname: Modarressi fullname: Modarressi, Ali organization: University of Geneva – sequence: 8 givenname: Olivier surname: Preynat‐Seauve fullname: Preynat‐Seauve, Olivier email: olivier.preynat-seauve@hcuge.ch organization: University of Geneva |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/35150064$$D View this record in MEDLINE/PubMed |
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| CitedBy_id | crossref_primary_10_1093_stcltm_szac081 crossref_primary_10_1097_JS9_0000000000002100 crossref_primary_10_1002_adhm_202304058 crossref_primary_10_3390_cells14151211 crossref_primary_10_1002_adhm_202500627 crossref_primary_10_1096_fj_202202117R crossref_primary_10_1007_s00266_024_04338_x crossref_primary_10_1016_j_cej_2022_141020 crossref_primary_10_1093_bbb_zbaf094 crossref_primary_10_3390_cells12071001 |
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| Copyright | 2022 The Authors. published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. 2022 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. 2022. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. |
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| Keywords | adipose-derived stem cells fat transplantation spheroids regenerative medicine |
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| SubjectTerms | Adipocytes Adipose Tissue - metabolism adipose‐derived stem cells Animal models Animals Autografts Autophagy Cell suspensions fat transplantation Gene expression Original Peptides Proteomes Reconstructive surgery regenerative medicine Software Spheroids Stem cell transplantation Stem Cells Suspensions Tissue engineering |
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| Title | Adipose‐derived stem cell spheroids are superior to single‐cell suspensions to improve fat autograft long‐term survival |
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