Faecal microbiota profile of Crohn’s disease determined by terminal restriction fragment length polymorphism analysis

Summary Background  Terminal restriction fragment length polymorphism (T‐RFLP) analyses are powerful tools to assess the diversity of complex microbiota. T‐RFLPs permit rapid comparisons of microbiota from many samples. Aim  To perform T‐RFLP analyses of faecal microbiota in Crohn’s disease (CD) pat...

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Bibliographic Details
Published in:Alimentary pharmacology & therapeutics Vol. 29; no. 1; pp. 75 - 82
Main Authors: ANDOH, A., TSUJIKAWA, T., SASAKI, M., MITSUYAMA, K., SUZUKI, Y., MATSUI, T., MATSUMOTO, T., BENNO, Y., FUJIYAMA, Y.
Format: Journal Article
Language:English
Published: Oxford, UK Blackwell Publishing Ltd 01.01.2009
Blackwell
Subjects:
Adult
Area Under Curve
Biological and medical sciences
Case-Control Studies
Crohn Disease - microbiology
Digestive system
DNA, Bacterial - analysis
Feces - microbiology
Female
Gastroenterology. Liver. Pancreas. Abdomen
Humans
Male
Medical sciences
Metagenome - genetics
Other diseases. Semiology
Pharmacology. Drug treatments
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Sequence Analysis, DNA - methods
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Young Adult
Crohn disease
Restriction fragment length polymorphism
Genetics
Digestive diseases
Intestinal disease
Microflora
Feces
Molecular biology
Inflammatory disease
ISSN:0269-2813, 1365-2036, 1365-2036
Online Access:Get full text
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Summary:Summary Background  Terminal restriction fragment length polymorphism (T‐RFLP) analyses are powerful tools to assess the diversity of complex microbiota. T‐RFLPs permit rapid comparisons of microbiota from many samples. Aim  To perform T‐RFLP analyses of faecal microbiota in Crohn’s disease (CD) patients to investigate potential alterations in faecal microbial communities and furthermore to analyse the effects of elemental diet on faecal microbiota profiles. Methods  Thirty‐four patients with CD and 30 healthy individuals were enrolled in the study. DNA was extracted from stool samples and 16S rRNA genes were amplified by PCR. PCR products were digested with BslI restriction enzymes and T‐RF lengths were determined. Results  Faecal microbial communities were classified into seven clusters. Almost all healthy individuals (28/30) were included in cluster I, II and III, but the majority of CD patients (25/34) could be divided into another four clusters (cluster IV–VII). Prediction of bacteria based on the BslI‐digested T‐RFLP database showed a significant decrease in Clostridium cluster IV, Clostridium cluster XI and subcluster XIVa in CD patients. In contrast, Bacteroides significantly increased in CD patients. Significant increases in Enterobacteriales were also observed in CD patients. Furthermore, elemental diets modulated faecal bacterial communities in CD patients. Conclusions  Terminal restriction fragment length polymorphism analyses showed that the diversity of faecal microbiota in patients with CD differed from that of healthy individuals. Furthermore, elemental diets modulated faecal microbiota composition, and this effect may be involved in mechanisms of clinical effects of elemental diet.
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ISSN:0269-2813
1365-2036
1365-2036
DOI:10.1111/j.1365-2036.2008.03860.x