Heterogeneous differentiation patterns of individual CD8+ T cells

Upon infection, antigen-specific CD8(+) T lymphocyte responses display a highly reproducible pattern of expansion and contraction that is thought to reflect a uniform behavior of individual cells. We tracked the progeny of individual mouse CD8(+) T cells by in vivo lineage tracing and demonstrated t...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) Vol. 340; no. 6132; p. 635
Main Authors: Gerlach, Carmen, Rohr, Jan C, Perié, Leïla, van Rooij, Nienke, van Heijst, Jeroen W J, Velds, Arno, Urbanus, Jos, Naik, Shalin H, Jacobs, Heinz, Beltman, Joost B, de Boer, Rob J, Schumacher, Ton N M
Format: Journal Article
Language:English
Published: United States 03.05.2013
Subjects:
Adoptive Transfer
Animals
Asymmetric Cell Division
CD8-Positive T-Lymphocytes - cytology
CD8-Positive T-Lymphocytes - immunology
Cell Differentiation
Cell Lineage
Cell Proliferation
Immunity, Cellular
Immunologic Memory
Immunophenotyping
Listeria monocytogenes
Listeriosis - immunology
Mice
Mice, Inbred C57BL
Mice, Transgenic
Models, Immunological
Receptors, Antigen, T-Cell - immunology
Single-Cell Analysis
Stochastic Processes
T-Lymphocyte Subsets - cytology
T-Lymphocyte Subsets - immunology
ISSN:1095-9203, 1095-9203
Online Access:Get more information
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Summary:Upon infection, antigen-specific CD8(+) T lymphocyte responses display a highly reproducible pattern of expansion and contraction that is thought to reflect a uniform behavior of individual cells. We tracked the progeny of individual mouse CD8(+) T cells by in vivo lineage tracing and demonstrated that, even for T cells bearing identical T cell receptors, both clonal expansion and differentiation patterns are heterogeneous. As a consequence, individual naïve T lymphocytes contributed differentially to short- and long-term protection, as revealed by participation of their progeny during primary versus recall infections. The discordance in fate of individual naïve T cells argues against asymmetric division as a singular driver of CD8(+) T cell heterogeneity and demonstrates that reproducibility of CD8(+) T cell responses is achieved through population averaging.
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ISSN:1095-9203
1095-9203
DOI:10.1126/science.1235487