Complete genome sequence of a novel iflavirus from wheat sawfly (Dolerus tritici)

Little is known about the insect viruses in wheat sawfly, Dolerus tritici , which is an important agricultural insect feeding on wheat leaves. Here, we used RNA sequencing to identify a novel single positive-strand RNA virus from the larvae of wheat sawfly collected in northern China and then determ...

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Bibliographic Details
Published in:	Archives of virology Vol. 170; no. 1; p. 21
Main Authors:	Guo, Jiashu, Liu, Wenwen, Chen, Chen, Xu, Zhongtian, Francis, Frederic, Wang, Xifeng
Format:	Journal Article
Language:	English
Published:	Vienna Springer Vienna 01.01.2025 Springer Nature B.V Springer
Subjects:	Agriculture & agronomie Agriculture & agronomy Amino Acid Sequence amino acids Animals Annotated Sequence Record Base Sequence Bats Biomedical and Life Sciences Biomedicine Capsids China Coat protein coat proteins Deformed wing virus family genome Genome, Viral Genomes Genomic analysis genus Hymenoptera Hymenoptera - virology Iflaviridae Infectious Diseases Insect Viruses Insect Viruses - classification Insect Viruses - genetics Insect Viruses - isolation & purification Larva Larva - virology Life sciences Medical Microbiology messenger RNA Nucleotide sequence nucleotide sequences Open Reading Frames Paralysis Phylogeny Plant viruses polyproteins RNA Viruses RNA Viruses - classification RNA Viruses - genetics RNA Viruses - isolation & purification RNA, Viral RNA, Viral - genetics sawflies Sciences du vivant sequence analysis Slow bee paralysis virus Triticum Triticum - virology Viral Proteins Viral Proteins - genetics Virology Viruses wheat Whole Genome Sequencing China
ISSN:	0304-8608, 1432-8798, 1432-8798
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Summary:	Little is known about the insect viruses in wheat sawfly, Dolerus tritici , which is an important agricultural insect feeding on wheat leaves. Here, we used RNA sequencing to identify a novel single positive-strand RNA virus from the larvae of wheat sawfly collected in northern China and then determined its complete genome sequence by rapid amplification of cDNA ends. The complete genome is 9,594 nt in length, including a polyA tail at its 3′ terminus, and it is predicted to encode a 326.3-kDa polyprotein. Phylogenetic analysis based on deduced amino acid sequences of the polyprotein revealed that this RNA virus clustered in a clade with deformed wing virus of the genus Iflavirus , family Iflaviridae. The full genome of this RNA virus shows 42.0–50.0% sequence identity with other iflaviruses. Comparisons of amino acid sequences showed that the coat protein of this RNA virus is most similar to that of slow bee paralysis virus, with 33.6% identity, suggesting that this virus is a new member in the genus Iflavirus . Thus, we have tentatively designated it as “Dolerus tritici iflavirus 1” (DtIV1). To our knowledge, this is the first report of an insect virus in wheat sawfly.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 scopus-id:2-s2.0-85212305042
ISSN:	0304-8608 1432-8798 1432-8798
DOI:	10.1007/s00705-024-06206-0