Detection of blaCTX-M and blaDHA genes in stool samples of healthy people: comparison of culture- and shotgun metagenomic-based approaches

We implemented culture- and shotgun metagenomic sequencing (SMS)-based methods to assess the gut colonization with extended-spectrum cephalosporin-resistant Enterobacterales (ESC-R- Ent ) in 42 volunteers. Both methods were performed using native and pre-enriched (broth supplemented with cefuroxime)...

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Bibliographic Details
Published in:Frontiers in microbiology Vol. 14; p. 1236208
Main Authors: Campos-Madueno, Edgar I., Aldeia, Claudia, Perreten, Vincent, Sendi, Parham, Moser, Aline I., Endimiani, Andrea
Format: Journal Article
Language:English
Published: Frontiers Media S.A 31.08.2023
Subjects:
AmpC
enrichment
ESBL
Illumina
metagenomics
Microbiology
stool
ISSN:1664-302X, 1664-302X
Online Access:Get full text
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