A point mutation in the zinc-finger transcription factor CqLOL1 controls the green flesh color in chieh-qua (Benincasa hispida Cogn. var. Chieh-qua How)

Flesh color is an essential trait in chieh-qua ( Cogn. var. How); however, the inheritance and molecular basis of green flesh trait remain unclear. In the present study, two F populations, derived from 1742 (white flesh) × FJ3211 (green flesh) and J16 (white flesh) × FJ5 (green flesh), were used to...

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Vydáno v:Frontiers in plant science Ročník 15; s. 1388115
Hlavní autoři: Peng, Jiazhu, Gao, Yin, Qiao, Yanchun, Wang, Guoping
Médium: Journal Article
Jazyk:angličtina
Vydáno: Switzerland Frontiers Media SA 21.10.2024
Frontiers Media S.A
Témata:
Agronomy
Amino acid substitution
Amino acids
Benincasa hispida
Carotenoids
chieh-qua
Chlorophyll
chlorophyll content
Chromosomes
Color
fine-mapping
flesh color
Fruits
Gene mapping
Genes
Genetic analysis
Genomes
Linkage analysis
Loci
Mutation
Normal distribution
Plant reproduction
Plant Science
Point mutation
Population studies
Proteins
Quantitative trait loci
Recombinants
Transcription factors
Zinc
Zinc finger proteins
zinc-finger transcription factor LOL1
chlorophyll content
flesh color
zinc-finger transcription factor LOL1
chieh-qua
fine-mapping
ISSN:1664-462X, 1664-462X
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Shrnutí:Flesh color is an essential trait in chieh-qua ( Cogn. var. How); however, the inheritance and molecular basis of green flesh trait remain unclear. In the present study, two F populations, derived from 1742 (white flesh) × FJ3211 (green flesh) and J16 (white flesh) × FJ5 (green flesh), were used to identify the green flesh ( ) locus. Genetic analysis revealed that the presence of green flesh was a quantitative trait that closely followed a normal distribution. Combining the results from QTL mapping and BSA-seq analysis, the locus was preliminarily determined to be located on chromosome 05 and was narrowed down to a 2.55-Mb interval by linkage analysis. A large J16 × FJ5 F population comprising 3,180 individuals was subsequently used to screen the recombinants, and the locus was fine-mapped to a region of 329.70 kb that harbors six genes. One of the candidate genes, , the zinc-finger transcription factor LOL1 (lsd one like 1 protein; ), was the strongest candidate gene for the locus according to sequence variation and expression analysis. Additionally, a point mutation (A > C) in resulted in the substitution of threonine (T) with proline (P) in the amino acid sequence, showing a complete relationship linked with flesh color in a panel of 45 germplasms. The study suggests that promotes the accumulation of chlorophyll content in chieh-qua and lead to green flesh. Our findings establish a theoretical and technical foundation for breeding different flesh color lines and elucidating the underlying mechanisms of flesh color in chieh-qua.
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Shi Liu, Northeast Agricultural University, China
Kang Wei, Chinese Academy of Agricultural Sciences, China
Reviewed by: Shyam Gundu, West Virginia State University, United States
These authors have contributed equally to this work
Edited by: Kaijing Zuo, Shanghai Jiao Tong University, China
ISSN:1664-462X
1664-462X
DOI:10.3389/fpls.2024.1388115