Homogeneous reporter system enables quantitative functional assessment of multiple transcription factors

We developed a high-content reporter system that allows quantitative assessment of activities of multiple transcription factors (TFs) in a eukaryotic cell. The system comprises a library of reporter constructs that are evaluated according to their transcription rates. All reporters produce essential...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Nature methods Jg. 5; H. 3; S. 253 - 260
Hauptverfasser:	Romanov, Sergei, Medvedev, Alexander, Gambarian, Maria, Poltoratskaya, Natalia, Moeser, Matt, Medvedeva, Liubov, Gambarian, Mikhail, Diatchenko, Luda, Makarov, Sergei
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	New York Nature Publishing Group US 01.03.2008 Nature Publishing Group
Schlagworte:	Animals Bioinformatics Biological Microscopy Biological Techniques Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biomedical research Carbazoles - pharmacology Cell Line, Tumor Cellular biology Cellular signal transduction Colforsin - pharmacology DNA binding proteins Gene Expression Profiling - methods Gene Library Genes, Reporter - physiology Genetic aspects Genetic regulation Homogeneity Humans Indoles - pharmacology Life Sciences Oligonucleotide Array Sequence Analysis - methods Physiological aspects Polymerase Chain Reaction Proteomics Rats Receptors, Aryl Hydrocarbon - drug effects Reproducibility of Results Research methodology Signal transduction Transcription Factors - genetics Transfection - methods Transforming Growth Factor beta - pharmacology United States
ISSN:	1548-7091, 1548-7105, 1548-7105
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Abstract	We developed a high-content reporter system that allows quantitative assessment of activities of multiple transcription factors (TFs) in a eukaryotic cell. The system comprises a library of reporter constructs that are evaluated according to their transcription rates. All reporters produce essentially identical messages that are subjected to 'processing', which generates a spectrum of distinguishable fragments that are analyzed quantitatively. The homogeneity of the reporter library afforded inherently uniform detection conditions for all reporters and provided repeatability, accuracy and robustness of assessment. We showed that this technology can be used to identify pathways transmitting cell responses to inducers, and that the profile of TF activities generated using this system represents a stable and sustained cell signature that clearly distinguishes different cell types and pathological conditions. This technology provides a framework for functional characterization of signal transduction networks through profiling activities of multiple TFs.
AbstractList	We developed a high-content reporter system that allows quantitative assessment of activities of multiple transcription factors (TFs) in a eukaryotic cell. The system comprises a library of reporter constructs that are evaluated according to their transcription rates. All reporters produce essentially identical messages that are subjected to 'processing', which generates a spectrum of distinguishable fragments that are analyzed quantitatively. The homogeneity of the reporter library afforded inherently uniform detection conditions for all reporters and provided repeatability, accuracy and robustness of assessment. We showed that this technology can be used to identify pathways transmitting cell responses to inducers, and that the profile of TF activities generated using this system represents a stable and sustained cell signature that clearly distinguishes different cell types and pathological conditions. This technology provides a framework for functional characterization of signal transduction networks through profiling activities of multiple TFs. We developed a high-content reporter system that allows quantitative assessment of activities of multiple transcription factors (TFs) in a eukaryotic cell. The system comprises a library of reporter constructs that are evaluated according to their transcription rates. All reporters produce essentially identical messages that are subjected to 'processing', which generates a spectrum of distinguishable fragments that are analyzed quantitatively. The homogeneity of the reporter library afforded inherently uniform detection conditions for all reporters and provided repeatability, accuracy and robustness of assessment. We showed that this technology can be used to identify pathways transmitting cell responses to inducers, and that the profile of TF activities generated using this system represents a stable and sustained cell signature that clearly distinguishes different cell types and pathological conditions. This technology provides a framework for functional characterization of signal transduction networks through profiling activities of multiple TFs. [PUBLICATION ABSTRACT] We developed a high-content reporter system that allows quantitative assessment of activities of multiple transcription factors (TFs) in a eukaryotic cell. The system comprises a library of reporter constructs that are evaluated according to their transcription rates. All reporters produce essentially identical messages that are subjected to 'processing', which generates a spectrum of distinguishable fragments that are analyzed quantitatively. The homogeneity of the reporter library afforded inherently uniform detection conditions for all reporters and provided repeatability, accuracy and robustness of assessment. We showed that this technology can be used to identify pathways transmitting cell responses to inducers, and that the profile of TF activities generated using this system represents a stable and sustained cell signature that clearly distinguishes different cell types and pathological conditions. This technology provides a framework for functional characterization of signal transduction networks through profiling activities of multiple TFs.We developed a high-content reporter system that allows quantitative assessment of activities of multiple transcription factors (TFs) in a eukaryotic cell. The system comprises a library of reporter constructs that are evaluated according to their transcription rates. All reporters produce essentially identical messages that are subjected to 'processing', which generates a spectrum of distinguishable fragments that are analyzed quantitatively. The homogeneity of the reporter library afforded inherently uniform detection conditions for all reporters and provided repeatability, accuracy and robustness of assessment. We showed that this technology can be used to identify pathways transmitting cell responses to inducers, and that the profile of TF activities generated using this system represents a stable and sustained cell signature that clearly distinguishes different cell types and pathological conditions. This technology provides a framework for functional characterization of signal transduction networks through profiling activities of multiple TFs.
Audience	Academic
Author	Gambarian, Maria Diatchenko, Luda Medvedev, Alexander Romanov, Sergei Makarov, Sergei Poltoratskaya, Natalia Medvedeva, Liubov Moeser, Matt Gambarian, Mikhail
Author_xml	– sequence: 1 givenname: Sergei surname: Romanov fullname: Romanov, Sergei organization: Attagene Inc – sequence: 2 givenname: Alexander surname: Medvedev fullname: Medvedev, Alexander organization: Attagene Inc – sequence: 3 givenname: Maria surname: Gambarian fullname: Gambarian, Maria organization: Attagene Inc – sequence: 4 givenname: Natalia surname: Poltoratskaya fullname: Poltoratskaya, Natalia organization: Attagene Inc – sequence: 5 givenname: Matt surname: Moeser fullname: Moeser, Matt organization: Attagene Inc – sequence: 6 givenname: Liubov surname: Medvedeva fullname: Medvedeva, Liubov organization: Attagene Inc – sequence: 7 givenname: Mikhail surname: Gambarian fullname: Gambarian, Mikhail organization: MishaGam Software – sequence: 8 givenname: Luda surname: Diatchenko fullname: Diatchenko, Luda organization: Center for Neurosensory Disorders, 2120 Old Dental Building, CB 7450, University of North Carolina at Chapel Hill – sequence: 9 givenname: Sergei surname: Makarov fullname: Makarov, Sergei email: smak@attagene.com organization: Attagene Inc
BackLink	https://www.ncbi.nlm.nih.gov/pubmed/18297081$$D View this record in MEDLINE/PubMed
BookMark	eNqF0s1rFDEUAPBBKvZDL_4BEhQ8WHbN50zmWIraQsGLnkMmedmmzCTbJCP0vzez2yqWBckhIfzeCy_vnTZHIQZomrcErwlm8nOYoNyuCZHti-aECC5XHcHi6OmMe3LcnOZ8hzFjnIpXzTGRtO-wJCfN7VWc4gYCxDmjBNuYCiSUH3KBCUHQwwgZ3c86FF908b8AuTmY4mPQI9I5Q84ThIKiQ9M8Fr8dAZWkQzbJbxeGnDYlpvy6een0mOHN437W_Pz65cfl1erm-7fry4ubleG8LSsuLW4lw9S2lBLmQDrQ0IpOCsEwtj3uWtByGJjtmaPMgmDdYKyh1rakJ-ys-bjPu03xfoZc1OSzgXHUuxpVhxnnvKP_hRQLIalc4Ptn8C7OqdZfDWWUSyIW9GGPNnoE5YOL9RfMklFd1MZIwUXLq1ofUHVZmLypXXW-3v8T8O7x7XmYwKpt8pNOD-qpgRXgPTAp5pzAKbNrVAw1sx8VwWqZEbWbEbXMSA359CzkT9ZD-HyPc0VhA-lv8Qf0bxZRzN8
CitedBy_id	crossref_primary_10_1289_EHP5297 crossref_primary_10_1002_etc_4869 crossref_primary_10_1039_C6IB00093B crossref_primary_10_1002_etc_4949 crossref_primary_10_1093_toxsci_kfad082 crossref_primary_10_1093_toxsci_kfx231 crossref_primary_10_1021_envhealth_4c00172 crossref_primary_10_1016_j_chemosphere_2023_140329 crossref_primary_10_1186_1471_2164_12_115 crossref_primary_10_1016_j_tox_2021_152789 crossref_primary_10_1002_bit_22916 crossref_primary_10_1371_journal_pone_0014026 crossref_primary_10_1016_j_cels_2024_11_003 crossref_primary_10_3390_toxics12040271 crossref_primary_10_3390_ijms232112718 crossref_primary_10_1016_j_yrtph_2023_105491 crossref_primary_10_1007_s00216_013_6712_9 crossref_primary_10_1016_j_comtox_2025_100376 crossref_primary_10_3390_ijms26072879 crossref_primary_10_1016_j_tips_2020_02_005 crossref_primary_10_1093_toxsci_kfw092 crossref_primary_10_1016_j_envint_2020_106339 crossref_primary_10_1016_j_tiv_2020_104913 crossref_primary_10_1080_10408444_2023_2231033 crossref_primary_10_1016_j_watres_2015_05_049 crossref_primary_10_1016_j_comtox_2021_100212 crossref_primary_10_5402_2011_806789 crossref_primary_10_1016_j_bcp_2014_05_023 crossref_primary_10_1016_j_ecoenv_2022_113330 crossref_primary_10_1073_pnas_1222805110 crossref_primary_10_1016_j_biopha_2024_117674 crossref_primary_10_1038_s41598_022_07031_8 crossref_primary_10_1586_epr_09_74 crossref_primary_10_1016_j_tiv_2020_105016 crossref_primary_10_1038_nmeth_1407 crossref_primary_10_1080_10937404_2025_2521615 crossref_primary_10_1093_nar_gku142 crossref_primary_10_1016_j_molimm_2009_04_008 crossref_primary_10_3389_fphar_2021_630457 crossref_primary_10_1074_jbc_M114_621995 crossref_primary_10_1016_j_chembiol_2017_06_003 crossref_primary_10_1126_sciadv_aar4666 crossref_primary_10_1289_ehp_1510352 crossref_primary_10_1186_1471_2199_10_20 crossref_primary_10_1016_j_envpol_2021_117928 crossref_primary_10_1016_j_yrtph_2023_105391 crossref_primary_10_1371_journal_pone_0050521 crossref_primary_10_1289_ehp_1002180 crossref_primary_10_1080_10408444_2018_1493086 crossref_primary_10_1111_biom_12114 crossref_primary_10_1016_j_yrtph_2014_09_009 crossref_primary_10_1016_j_jhazmat_2023_132312 crossref_primary_10_1016_j_pain_2011_09_005 crossref_primary_10_1073_pnas_2420011122 crossref_primary_10_1089_aivt_2014_0006 crossref_primary_10_1016_j_envint_2013_11_012 crossref_primary_10_1021_es102150z crossref_primary_10_1021_tx400343t crossref_primary_10_1093_carcin_bgv037
Cites_doi	10.1126/science.275.5307.1784 10.1177/1087057103261446 10.1126/science.275.5307.1787 10.1038/73432 10.1073/pnas.95.25.14863 10.1038/381713a0 10.1101/gad.3.7.1019 10.1128/MCB.12.3.1387 10.1093/nar/gkj143 10.1093/emboj/17.11.3091 10.1002/j.1460-2075.1991.tb07709.x 10.1128/MCB.17.7.3629 10.1158/0008-5472.CAN-04-0107 10.1016/S1043-2760(01)00527-6 10.1089/adt.2006.4.333 10.1006/abio.1994.1254 10.1002/(SICI)1098-2744(199903)24:3<153::AID-MC1>3.0.CO;2-P 10.1006/abio.1997.2231 10.1016/j.sbi.2004.05.004 10.1093/toxsci/kfi154 10.1038/35021093 10.1038/labinvest.3700166 10.1016/0006-291X(92)92290-E 10.1038/328175a0 10.1016/S0378-1119(01)00350-X 10.1126/science.276.5316.1268 10.1038/nbt1239 10.1016/S1016-8478(23)15111-9
ContentType	Journal Article
Copyright	Springer Nature America, Inc. 2008 COPYRIGHT 2008 Nature Publishing Group Copyright Nature Publishing Group Mar 2008
Copyright_xml	– notice: Springer Nature America, Inc. 2008 – notice: COPYRIGHT 2008 Nature Publishing Group – notice: Copyright Nature Publishing Group Mar 2008
DBID	AAYXX CITATION CGR CUY CVF ECM EIF NPM 3V. 7QL 7QO 7SS 7TK 7U9 7X2 7X7 7XB 88E 88I 8AO 8FD 8FE 8FG 8FH 8FI 8FJ 8FK ABJCF ABUWG AEUYN AFKRA ARAPS ATCPS AZQEC BBNVY BENPR BGLVJ BHPHI BKSAR C1K CCPQU D1I DWQXO FR3 FYUFA GHDGH GNUQQ H94 HCIFZ K9. KB. L6V LK8 M0K M0S M1P M2P M7N M7P M7S P5Z P62 P64 PATMY PCBAR PDBOC PHGZM PHGZT PJZUB PKEHL PPXIY PQEST PQGLB PQQKQ PQUKI PRINS PTHSS PYCSY Q9U RC3 7TM 7X8
DOI	10.1038/nmeth.1186
DatabaseName	CrossRef Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed ProQuest Central (Corporate) Bacteriology Abstracts (Microbiology B) Biotechnology Research Abstracts Entomology Abstracts (Full archive) Neurosciences Abstracts Virology and AIDS Abstracts Agricultural Science Collection Health & Medical Collection (ProQuest) ProQuest Central (purchase pre-March 2016) Medical Database (Alumni Edition) Science Database (Alumni Edition) ProQuest Pharma Collection Technology Research Database ProQuest SciTech Collection ProQuest Technology Collection ProQuest Natural Science Collection ProQuest Hospital Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) Materials Science & Engineering Collection ProQuest Central (Alumni) ProQuest One Sustainability ProQuest Central UK/Ireland Advanced Technologies & Computer Science Collection Agricultural & Environmental Science Collection ProQuest Central Essentials Biological Science Collection ProQuest Central Technology Collection Natural Science Collection Earth, Atmospheric & Aquatic Science Collection Environmental Sciences and Pollution Management ProQuest One ProQuest Materials Science Collection ProQuest Central Engineering Research Database Proquest Health Research Premium Collection Health Research Premium Collection (Alumni) ProQuest Central Student AIDS and Cancer Research Abstracts SciTech Premium Collection ProQuest Health & Medical Complete (Alumni) Materials Science Database ProQuest Engineering Collection ProQuest Biological Science Collection Agricultural Science Database ProQuest Health & Medical Collection Medical Database Science Database Algology Mycology and Protozoology Abstracts (Microbiology C) Biological Science Database Engineering Database Advanced Technologies & Aerospace Database ProQuest Advanced Technologies & Aerospace Collection Biotechnology and BioEngineering Abstracts Environmental Science Database Earth, Atmospheric & Aquatic Science Database Materials Science Collection ProQuest Central Premium ProQuest One Academic (New) ProQuest Health & Medical Research Collection ProQuest One Academic Middle East (New) ProQuest One Health & Nursing ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Applied & Life Sciences ProQuest One Academic (retired) ProQuest One Academic UKI Edition ProQuest Central China Engineering Collection Environmental Science Collection ProQuest Central Basic Genetics Abstracts Nucleic Acids Abstracts MEDLINE - Academic
DatabaseTitle	CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) Agricultural Science Database ProQuest Central Student ProQuest Advanced Technologies & Aerospace Collection ProQuest Central Essentials SciTech Premium Collection ProQuest Central China Environmental Sciences and Pollution Management ProQuest One Applied & Life Sciences ProQuest One Sustainability Health Research Premium Collection Natural Science Collection Health & Medical Research Collection Biological Science Collection ProQuest Central (New) ProQuest Medical Library (Alumni) Engineering Collection Advanced Technologies & Aerospace Collection Engineering Database Virology and AIDS Abstracts ProQuest Science Journals (Alumni Edition) ProQuest Biological Science Collection ProQuest One Academic Eastern Edition Earth, Atmospheric & Aquatic Science Database Agricultural Science Collection ProQuest Hospital Collection ProQuest Technology Collection Health Research Premium Collection (Alumni) Biological Science Database Neurosciences Abstracts ProQuest Hospital Collection (Alumni) Biotechnology and BioEngineering Abstracts Environmental Science Collection Entomology Abstracts ProQuest Health & Medical Complete ProQuest One Academic UKI Edition Environmental Science Database Engineering Research Database ProQuest One Academic ProQuest One Academic (New) Technology Collection Technology Research Database ProQuest One Academic Middle East (New) Materials Science Collection ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) ProQuest One Community College ProQuest One Health & Nursing ProQuest Natural Science Collection ProQuest Pharma Collection ProQuest Central Earth, Atmospheric & Aquatic Science Collection ProQuest Health & Medical Research Collection Genetics Abstracts ProQuest Engineering Collection Biotechnology Research Abstracts Health and Medicine Complete (Alumni Edition) ProQuest Central Korea Bacteriology Abstracts (Microbiology B) Algology Mycology and Protozoology Abstracts (Microbiology C) Agricultural & Environmental Science Collection AIDS and Cancer Research Abstracts Materials Science Database ProQuest Materials Science Collection ProQuest Central Basic ProQuest Science Journals ProQuest SciTech Collection Advanced Technologies & Aerospace Database ProQuest Medical Library Materials Science & Engineering Collection ProQuest Central (Alumni) Nucleic Acids Abstracts MEDLINE - Academic
DatabaseTitleList	MEDLINE Agricultural Science Database Nucleic Acids Abstracts MEDLINE - Academic
Database_xml	– sequence: 1 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: BENPR name: ProQuest Central url: https://www.proquest.com/central sourceTypes: Aggregation Database
DeliveryMethod	fulltext_linktorsrc
Discipline	Biology
EISSN	1548-7105
EndPage	260
ExternalDocumentID	1438027631 A186854564 18297081 10_1038_nmeth_1186
Genre	Evaluation Studies Journal Article Research Support, N.I.H., Extramural
GeographicLocations	United States
GeographicLocations_xml	– name: United States
GrantInformation_xml	– fundername: NIAID NIH HHS grantid: UO1 AI061360 – fundername: NCI NIH HHS grantid: 1R43CA101636 – fundername: NCI NIH HHS grantid: 1R43CA101271
GroupedDBID	--- -~X 0R~ 123 29M 39C 3V. 4.4 53G 5BI 7X2 7X7 7XC 88E 88I 8AO 8CJ 8FE 8FG 8FH 8FI 8FJ 8R4 8R5 AAEEF AAHBH AARCD AAYZH AAZLF ABAWZ ABDBF ABJCF ABJNI ABLJU ABUWG ACBWK ACGFS ACGOD ACIWK ACPRK ACUHS ADBBV AENEX AEUYN AFANA AFBBN AFKRA AFRAH AFSHS AGAYW AHBCP AHMBA AHSBF AIBTJ ALFFA ALIPV ALMA_UNASSIGNED_HOLDINGS ARAPS ARMCB ASPBG ATCPS AVWKF AXYYD AZFZN AZQEC BBNVY BENPR BGLVJ BHPHI BKKNO BKSAR BPHCQ BVXVI CCPQU CS3 D1I D1J D1K DB5 DU5 DWQXO EBS EE. EJD EMOBN ESX F5P FEDTE FSGXE FYUFA FZEXT GNUQQ HCIFZ HMCUK HVGLF HZ~ IAO IHR INH INR ITC K6- KB. L6V LK5 LK8 M0K M1P M2P M7P M7R M7S NNMJJ O9- ODYON P2P P62 PATMY PCBAR PDBOC PQQKQ PROAC PSQYO PTHSS PYCSY Q2X RNS RNT RNTTT SHXYY SIXXV SJN SNYQT SOJ SV3 TAOOD TBHMF TDRGL TSG TUS UKHRP ~8M AAYXX AFFHD ATHPR CITATION PHGZM PHGZT PJZUB PPXIY PQGLB CGR CUY CVF ECM EIF NPM 7QL 7QO 7SS 7TK 7U9 7XB 8FD 8FK C1K FR3 H94 K9. M7N P64 PKEHL PQEST PQUKI PRINS Q9U RC3 7TM PUEGO 7X8
ID	FETCH-LOGICAL-c446t-48d068302d62213fe8feae657855300d9076ea8bb3d93f23de537bcdc2dd61913
IEDL.DBID	M2P
ISICitedReferencesCount	81
ISICitedReferencesURI	http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000253777900019&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
ISSN	1548-7091 1548-7105
IngestDate	Sun Nov 09 11:35:37 EST 2025 Thu Oct 02 04:15:16 EDT 2025 Sun Nov 30 05:35:34 EST 2025 Tue Nov 11 11:09:18 EST 2025 Tue Nov 04 18:31:50 EST 2025 Wed Feb 19 01:51:17 EST 2025 Tue Nov 18 22:23:14 EST 2025 Sat Nov 29 02:49:23 EST 2025 Fri Feb 21 02:37:43 EST 2025
IsPeerReviewed	true
IsScholarly	true
Issue	3
Language	English
License	http://www.springer.com/tdm
LinkModel	DirectLink
MergedId	FETCHMERGED-LOGICAL-c446t-48d068302d62213fe8feae657855300d9076ea8bb3d93f23de537bcdc2dd61913
Notes	ObjectType-Article-1 SourceType-Scholarly Journals-1 content type line 14 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Undefined-1 ObjectType-Feature-3
PMID	18297081
PQID	223248152
PQPubID	28015
PageCount	8
ParticipantIDs	proquest_miscellaneous_70344472 proquest_miscellaneous_20558282 proquest_journals_223248152 gale_infotracmisc_A186854564 gale_infotracacademiconefile_A186854564 pubmed_primary_18297081 crossref_citationtrail_10_1038_nmeth_1186 crossref_primary_10_1038_nmeth_1186 springer_journals_10_1038_nmeth_1186
PublicationCentury	2000
PublicationDate	2008-03-01
PublicationDateYYYYMMDD	2008-03-01
PublicationDate_xml	– month: 03 year: 2008 text: 2008-03-01 day: 01
PublicationDecade	2000
PublicationPlace	New York
PublicationPlace_xml	– name: New York – name: United States
PublicationSubtitle	Techniques for life scientists and chemists
PublicationTitle	Nature methods
PublicationTitleAbbrev	Nat Methods
PublicationTitleAlternate	Nat Methods
PublicationYear	2008
Publisher	Nature Publishing Group US Nature Publishing Group
Publisher_xml	– name: Nature Publishing Group US – name: Nature Publishing Group
References	Guhaniyogi, Brewer (CR10) 2001; 265 Troester (CR26) 2004; 64 Ross (CR22) 2000; 24 Waldman, Lengauer, Kinzler, Vogelstein (CR27) 1996; 381 Okabe (CR21) 2001; 61 Goyette (CR28) 1992; 12 CR14 Kovacs, Kaplan (CR8) 1992; 189 Levitt, Briggs, Gil, Proudfoot (CR12) 1989; 3 Oberg, Bergander, Hakansson, Rannug, Rannug (CR15) 2005; 85 Bronstein, Fortin, Stanley, Stewart, Kricka (CR7) 1994; 219 Nakshatri, Bhat-Nakshatri, Martin, Goulet, Sledge (CR25) 1997; 17 Zhang (CR19) 1997; 276 Babu, Luscombe, Aravind, Gerstein, Teichmann (CR1) 2004; 14 Shao (CR29) 1995; 11 Benotmane, Hoylaerts, Collen, Belayew (CR4) 1997; 250 Shim, Karin (CR11) 2002; 14 Tamaru (CR31) 2004; 84 Perou (CR20) 2000; 406 Dennler (CR17) 1998; 17 Nuwaysir, Bittner, Trent, Barrett, Afshari (CR32) 1999; 24 Montminy, Bilezikjian (CR16) 1987; 328 Morin (CR24) 1997; 275 Korinek (CR23) 1997; 275 Matys (CR3) 2006; 34 Sugikawa (CR30) 1999; 19 Rosenau, Emery, Kaboord, Qoronfleh (CR5) 2004; 9 Hermanson, Glass, Rosenfeld (CR6) 2002; 13 Li, Jiang, Yaoi (CR9) 2006; 4 Enriquez-Harris, Levitt, Briggs, Proudfoot (CR13) 1991; 10 Eisen, Spellman, Brown, Botstein (CR18) 1998; 95 Stegmaier, Kel, Wingender (CR2) 2004; 15 P Enriquez-Harris (BFnmeth1186_CR13) 1991; 10 AM Benotmane (BFnmeth1186_CR4) 1997; 250 O Hermanson (BFnmeth1186_CR6) 2002; 13 X Li (BFnmeth1186_CR9) 2006; 4 N Tamaru (BFnmeth1186_CR31) 2004; 84 H Okabe (BFnmeth1186_CR21) 2001; 61 PJ Morin (BFnmeth1186_CR24) 1997; 275 BFnmeth1186_CR14 MR Montminy (BFnmeth1186_CR16) 1987; 328 V Korinek (BFnmeth1186_CR23) 1997; 275 MC Goyette (BFnmeth1186_CR28) 1992; 12 E Sugikawa (BFnmeth1186_CR30) 1999; 19 MM Babu (BFnmeth1186_CR1) 2004; 14 J Guhaniyogi (BFnmeth1186_CR10) 2001; 265 P Stegmaier (BFnmeth1186_CR2) 2004; 15 V Matys (BFnmeth1186_CR3) 2006; 34 L Zhang (BFnmeth1186_CR19) 1997; 276 MA Troester (BFnmeth1186_CR26) 2004; 64 EF Nuwaysir (BFnmeth1186_CR32) 1999; 24 CM Perou (BFnmeth1186_CR20) 2000; 406 ZM Shao (BFnmeth1186_CR29) 1995; 11 M Oberg (BFnmeth1186_CR15) 2005; 85 C Rosenau (BFnmeth1186_CR5) 2004; 9 H Nakshatri (BFnmeth1186_CR25) 1997; 17 T Waldman (BFnmeth1186_CR27) 1996; 381 DM Kovacs (BFnmeth1186_CR8) 1992; 189 S Dennler (BFnmeth1186_CR17) 1998; 17 J Shim (BFnmeth1186_CR11) 2002; 14 MB Eisen (BFnmeth1186_CR18) 1998; 95 N Levitt (BFnmeth1186_CR12) 1989; 3 I Bronstein (BFnmeth1186_CR7) 1994; 219 DT Ross (BFnmeth1186_CR22) 2000; 24
References_xml	– volume: 275 start-page: 1784 year: 1997 end-page: 1787 ident: CR23 article-title: Constitutive transcriptional activation by a beta-catenin-Tcf complex in APC−/− colon carcinoma publication-title: Science doi: 10.1126/science.275.5307.1784 – volume: 9 start-page: 334 year: 2004 end-page: 342 ident: CR5 article-title: Development of a high-throughput plate-based chemiluminescent transcription factor assay publication-title: J. Biomol. Screen. doi: 10.1177/1087057103261446 – volume: 275 start-page: 1787 year: 1997 end-page: 1790 ident: CR24 article-title: Activation of beta-catenin-Tcf signaling in colon cancer by mutations in beta-catenin or APC publication-title: Science doi: 10.1126/science.275.5307.1787 – volume: 24 start-page: 227 year: 2000 end-page: 235 ident: CR22 article-title: Systematic variation in gene expression patterns in human cancer cell lines publication-title: Nat. Genet. doi: 10.1038/73432 – ident: CR14 – volume: 95 start-page: 14863 year: 1998 end-page: 14868 ident: CR18 article-title: Cluster analysis and display of genome-wide expression patterns publication-title: Proc. Natl. Acad. Sci. USA doi: 10.1073/pnas.95.25.14863 – volume: 381 start-page: 713 year: 1996 end-page: 716 ident: CR27 article-title: Uncoupling of S phase and mitosis induced by anticancer agents in cells lacking p21 publication-title: Nature doi: 10.1038/381713a0 – volume: 3 start-page: 1019 year: 1989 end-page: 1025 ident: CR12 article-title: Definition of an efficient synthetic poly(A) site publication-title: Genes Dev. doi: 10.1101/gad.3.7.1019 – volume: 12 start-page: 1387 year: 1992 end-page: 1395 ident: CR28 article-title: Progression of colorectal cancer is associated with multiple tumor suppressor gene defects but inhibition of tumorigenicity is accomplished by correction of any single defect via chromosome transfer publication-title: Mol. Cell. Biol. doi: 10.1128/MCB.12.3.1387 – volume: 15 start-page: 276 year: 2004 end-page: 286 ident: CR2 article-title: Systematic DNA-binding domain classification of transcription factors publication-title: Genome Inform. – volume: 34 start-page: D108 year: 2006 end-page: D110 ident: CR3 article-title: TRANSFAC and its module TRANSCompel: transcriptional gene regulation in eukaryotes publication-title: Nucleic Acids Res. doi: 10.1093/nar/gkj143 – volume: 17 start-page: 3091 year: 1998 end-page: 3100 ident: CR17 article-title: Direct binding of Smad3 and Smad4 to critical TGF beta-inducible elements in the promoter of human plasminogen activator inhibitor-type 1 gene publication-title: EMBO J. doi: 10.1093/emboj/17.11.3091 – volume: 11 start-page: 493 year: 1995 end-page: 504 ident: CR29 article-title: p53 independent G0/G1 arrest and apoptosis induced by a novel retinoid in human breast cancer cells publication-title: Oncogene – volume: 61 start-page: 2129 year: 2001 end-page: 2137 ident: CR21 article-title: Genome-wide analysis of gene expression in human hepatocellular carcinomas using cDNA microarray: identification of genes involved in viral carcinogenesis and tumor progression publication-title: Cancer Res. – volume: 10 start-page: 1833 year: 1991 end-page: 1842 ident: CR13 article-title: A pause site for RNA polymerase II is associated with termination of transcription publication-title: EMBO J. doi: 10.1002/j.1460-2075.1991.tb07709.x – volume: 17 start-page: 3629 year: 1997 end-page: 3639 ident: CR25 article-title: Constitutive activation of NF-kappaB during progression of breast cancer to hormone-independent growth publication-title: Mol. Cell. Biol. doi: 10.1128/MCB.17.7.3629 – volume: 64 start-page: 4218 year: 2004 end-page: 4226 ident: CR26 article-title: Cell-type-specific responses to chemotherapeutics in breast cancer publication-title: Cancer Res. doi: 10.1158/0008-5472.CAN-04-0107 – volume: 13 start-page: 55 year: 2002 end-page: 60 ident: CR6 article-title: Nuclear receptor coregulators: multiple modes of modification publication-title: Trends Endocrinol. Metab. doi: 10.1016/S1043-2760(01)00527-6 – volume: 4 start-page: 333 year: 2006 end-page: 341 ident: CR9 article-title: High throughput assays for analyzing transcription factors publication-title: Assay Drug Dev. Technol. doi: 10.1089/adt.2006.4.333 – volume: 219 start-page: 169 year: 1994 end-page: 181 ident: CR7 article-title: Chemiluminescent and bioluminescent reporter gene assays publication-title: Anal. Biochem. doi: 10.1006/abio.1994.1254 – volume: 24 start-page: 153 year: 1999 end-page: 159 ident: CR32 article-title: Microarrays and toxicology: the advent of toxicogenomics publication-title: Mol. Carcinog. doi: 10.1002/(SICI)1098-2744(199903)24:3<153::AID-MC1>3.0.CO;2-P – volume: 250 start-page: 181 year: 1997 end-page: 185 ident: CR4 article-title: Nonisotopic quantitative analysis of protein-DNA interactions at equilibrium publication-title: Anal. Biochem. doi: 10.1006/abio.1997.2231 – volume: 14 start-page: 323 year: 2002 end-page: 331 ident: CR11 article-title: The control of mRNA stability in response to extracellular stimuli publication-title: Mol. Cells – volume: 14 start-page: 283 year: 2004 end-page: 291 ident: CR1 article-title: Structure and evolution of transcriptional regulatory networks publication-title: Curr. Opin. Struct. Biol. doi: 10.1016/j.sbi.2004.05.004 – volume: 19 start-page: 3099 year: 1999 end-page: 3108 ident: CR30 article-title: Mutant p53 mediated induction of cell cycle arrest and apoptosis at G1 phase by 9-hydroxyellipticine publication-title: Anticancer Res. – volume: 85 start-page: 935 year: 2005 end-page: 943 ident: CR15 article-title: Identification of the tryptophan photoproduct 6-formylindolo[3,2-b]carbazole, in cell culture medium, as a factor that controls the background aryl hydrocarbon receptor activity publication-title: Toxicol. Sci. doi: 10.1093/toxsci/kfi154 – volume: 406 start-page: 747 year: 2000 end-page: 752 ident: CR20 article-title: Molecular portraits of human breast tumours publication-title: Nature doi: 10.1038/35021093 – volume: 84 start-page: 1460 year: 2004 end-page: 1471 ident: CR31 article-title: Estrogen receptor-associated expression of keratinocyte growth factor and its possible role in the inhibition of apoptosis in human breast cancer publication-title: Lab. Invest. doi: 10.1038/labinvest.3700166 – volume: 189 start-page: 912 year: 1992 end-page: 918 ident: CR8 article-title: Discordant estimates of heterologous promoter activity as determined by reporter gene mRNA levels and enzyme activity publication-title: Biochem. Biophys. Res. Commun. doi: 10.1016/0006-291X(92)92290-E – volume: 328 start-page: 175 year: 1987 end-page: 178 ident: CR16 article-title: Binding of a nuclear protein to the cyclic-AMP response element of the somatostatin gene publication-title: Nature doi: 10.1038/328175a0 – volume: 265 start-page: 11 year: 2001 end-page: 23 ident: CR10 article-title: Regulation of mRNA stability in mammalian cells publication-title: Gene doi: 10.1016/S0378-1119(01)00350-X – volume: 276 start-page: 1268 year: 1997 end-page: 1272 ident: CR19 article-title: Gene expression profiles in normal and cancer cells publication-title: Science doi: 10.1126/science.276.5316.1268 – volume: 61 start-page: 2129 year: 2001 ident: BFnmeth1186_CR21 publication-title: Cancer Res. – volume: 328 start-page: 175 year: 1987 ident: BFnmeth1186_CR16 publication-title: Nature doi: 10.1038/328175a0 – volume: 11 start-page: 493 year: 1995 ident: BFnmeth1186_CR29 publication-title: Oncogene – volume: 95 start-page: 14863 year: 1998 ident: BFnmeth1186_CR18 publication-title: Proc. Natl. Acad. Sci. USA doi: 10.1073/pnas.95.25.14863 – volume: 12 start-page: 1387 year: 1992 ident: BFnmeth1186_CR28 publication-title: Mol. Cell. Biol. doi: 10.1128/MCB.12.3.1387 – volume: 24 start-page: 153 year: 1999 ident: BFnmeth1186_CR32 publication-title: Mol. Carcinog. doi: 10.1002/(SICI)1098-2744(199903)24:3<153::AID-MC1>3.0.CO;2-P – volume: 406 start-page: 747 year: 2000 ident: BFnmeth1186_CR20 publication-title: Nature doi: 10.1038/35021093 – volume: 275 start-page: 1784 year: 1997 ident: BFnmeth1186_CR23 publication-title: Science doi: 10.1126/science.275.5307.1784 – volume: 85 start-page: 935 year: 2005 ident: BFnmeth1186_CR15 publication-title: Toxicol. Sci. doi: 10.1093/toxsci/kfi154 – volume: 24 start-page: 227 year: 2000 ident: BFnmeth1186_CR22 publication-title: Nat. Genet. doi: 10.1038/73432 – volume: 15 start-page: 276 year: 2004 ident: BFnmeth1186_CR2 publication-title: Genome Inform. – volume: 381 start-page: 713 year: 1996 ident: BFnmeth1186_CR27 publication-title: Nature doi: 10.1038/381713a0 – volume: 219 start-page: 169 year: 1994 ident: BFnmeth1186_CR7 publication-title: Anal. Biochem. doi: 10.1006/abio.1994.1254 – volume: 3 start-page: 1019 year: 1989 ident: BFnmeth1186_CR12 publication-title: Genes Dev. doi: 10.1101/gad.3.7.1019 – volume: 19 start-page: 3099 year: 1999 ident: BFnmeth1186_CR30 publication-title: Anticancer Res. – volume: 275 start-page: 1787 year: 1997 ident: BFnmeth1186_CR24 publication-title: Science doi: 10.1126/science.275.5307.1787 – volume: 84 start-page: 1460 year: 2004 ident: BFnmeth1186_CR31 publication-title: Lab. Invest. doi: 10.1038/labinvest.3700166 – volume: 17 start-page: 3091 year: 1998 ident: BFnmeth1186_CR17 publication-title: EMBO J. doi: 10.1093/emboj/17.11.3091 – volume: 64 start-page: 4218 year: 2004 ident: BFnmeth1186_CR26 publication-title: Cancer Res. doi: 10.1158/0008-5472.CAN-04-0107 – volume: 189 start-page: 912 year: 1992 ident: BFnmeth1186_CR8 publication-title: Biochem. Biophys. Res. Commun. doi: 10.1016/0006-291X(92)92290-E – volume: 250 start-page: 181 year: 1997 ident: BFnmeth1186_CR4 publication-title: Anal. Biochem. doi: 10.1006/abio.1997.2231 – volume: 17 start-page: 3629 year: 1997 ident: BFnmeth1186_CR25 publication-title: Mol. Cell. Biol. doi: 10.1128/MCB.17.7.3629 – volume: 34 start-page: D108 year: 2006 ident: BFnmeth1186_CR3 publication-title: Nucleic Acids Res. doi: 10.1093/nar/gkj143 – volume: 9 start-page: 334 year: 2004 ident: BFnmeth1186_CR5 publication-title: J. Biomol. Screen. doi: 10.1177/1087057103261446 – volume: 13 start-page: 55 year: 2002 ident: BFnmeth1186_CR6 publication-title: Trends Endocrinol. Metab. doi: 10.1016/S1043-2760(01)00527-6 – volume: 14 start-page: 283 year: 2004 ident: BFnmeth1186_CR1 publication-title: Curr. Opin. Struct. Biol. doi: 10.1016/j.sbi.2004.05.004 – ident: BFnmeth1186_CR14 doi: 10.1038/nbt1239 – volume: 10 start-page: 1833 year: 1991 ident: BFnmeth1186_CR13 publication-title: EMBO J. doi: 10.1002/j.1460-2075.1991.tb07709.x – volume: 4 start-page: 333 year: 2006 ident: BFnmeth1186_CR9 publication-title: Assay Drug Dev. Technol. doi: 10.1089/adt.2006.4.333 – volume: 14 start-page: 323 year: 2002 ident: BFnmeth1186_CR11 publication-title: Mol. Cells doi: 10.1016/S1016-8478(23)15111-9 – volume: 265 start-page: 11 year: 2001 ident: BFnmeth1186_CR10 publication-title: Gene doi: 10.1016/S0378-1119(01)00350-X – volume: 276 start-page: 1268 year: 1997 ident: BFnmeth1186_CR19 publication-title: Science doi: 10.1126/science.276.5316.1268
SSID	ssj0033425
Score	2.2394288
Snippet	We developed a high-content reporter system that allows quantitative assessment of activities of multiple transcription factors (TFs) in a eukaryotic cell. The...
SourceID	proquest gale pubmed crossref springer
SourceType	Aggregation Database Index Database Enrichment Source Publisher
StartPage	253
SubjectTerms	Animals Bioinformatics Biological Microscopy Biological Techniques Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biomedical research Carbazoles - pharmacology Cell Line, Tumor Cellular biology Cellular signal transduction Colforsin - pharmacology DNA binding proteins Gene Expression Profiling - methods Gene Library Genes, Reporter - physiology Genetic aspects Genetic regulation Homogeneity Humans Indoles - pharmacology Life Sciences Oligonucleotide Array Sequence Analysis - methods Physiological aspects Polymerase Chain Reaction Proteomics Rats Receptors, Aryl Hydrocarbon - drug effects Reproducibility of Results Research methodology Signal transduction Transcription Factors - genetics Transfection - methods Transforming Growth Factor beta - pharmacology
Title	Homogeneous reporter system enables quantitative functional assessment of multiple transcription factors
URI	https://link.springer.com/article/10.1038/nmeth.1186 https://www.ncbi.nlm.nih.gov/pubmed/18297081 https://www.proquest.com/docview/223248152 https://www.proquest.com/docview/20558282 https://www.proquest.com/docview/70344472
Volume	5
WOSCitedRecordID	wos000253777900019&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
hasFullText	1
inHoldings	1
isFullTextHit
isPrint
journalDatabaseRights	– providerCode: PRVPQU databaseName: Advanced Technologies & Aerospace Database customDbUrl: eissn: 1548-7105 dateEnd: 20171231 omitProxy: false ssIdentifier: ssj0033425 issn: 1548-7091 databaseCode: P5Z dateStart: 20041001 isFulltext: true titleUrlDefault: https://search.proquest.com/hightechjournals providerName: ProQuest – providerCode: PRVPQU databaseName: Agricultural Science Database customDbUrl: eissn: 1548-7105 dateEnd: 20171231 omitProxy: false ssIdentifier: ssj0033425 issn: 1548-7091 databaseCode: M0K dateStart: 20041001 isFulltext: true titleUrlDefault: https://search.proquest.com/agriculturejournals providerName: ProQuest – providerCode: PRVPQU databaseName: Biological Science Database customDbUrl: eissn: 1548-7105 dateEnd: 20171231 omitProxy: false ssIdentifier: ssj0033425 issn: 1548-7091 databaseCode: M7P dateStart: 20041001 isFulltext: true titleUrlDefault: http://search.proquest.com/biologicalscijournals providerName: ProQuest – providerCode: PRVPQU databaseName: Earth, Atmospheric & Aquatic Science Database customDbUrl: eissn: 1548-7105 dateEnd: 20171231 omitProxy: false ssIdentifier: ssj0033425 issn: 1548-7091 databaseCode: PCBAR dateStart: 20041001 isFulltext: true titleUrlDefault: https://search.proquest.com/eaasdb providerName: ProQuest – providerCode: PRVPQU databaseName: Engineering Database customDbUrl: eissn: 1548-7105 dateEnd: 20171231 omitProxy: false ssIdentifier: ssj0033425 issn: 1548-7091 databaseCode: M7S dateStart: 20041001 isFulltext: true titleUrlDefault: http://search.proquest.com providerName: ProQuest – providerCode: PRVPQU databaseName: Environmental Science Database customDbUrl: eissn: 1548-7105 dateEnd: 20171231 omitProxy: false ssIdentifier: ssj0033425 issn: 1548-7091 databaseCode: PATMY dateStart: 20041001 isFulltext: true titleUrlDefault: http://search.proquest.com/environmentalscience providerName: ProQuest – providerCode: PRVPQU databaseName: Health & Medical Collection (ProQuest) customDbUrl: eissn: 1548-7105 dateEnd: 20171231 omitProxy: false ssIdentifier: ssj0033425 issn: 1548-7091 databaseCode: 7X7 dateStart: 20041001 isFulltext: true titleUrlDefault: https://search.proquest.com/healthcomplete providerName: ProQuest – providerCode: PRVPQU databaseName: Materials Science Database customDbUrl: eissn: 1548-7105 dateEnd: 20171231 omitProxy: false ssIdentifier: ssj0033425 issn: 1548-7091 databaseCode: KB. dateStart: 20041001 isFulltext: true titleUrlDefault: http://search.proquest.com/materialsscijournals providerName: ProQuest – providerCode: PRVPQU databaseName: ProQuest Central customDbUrl: eissn: 1548-7105 dateEnd: 20171231 omitProxy: false ssIdentifier: ssj0033425 issn: 1548-7091 databaseCode: BENPR dateStart: 20041001 isFulltext: true titleUrlDefault: https://www.proquest.com/central providerName: ProQuest – providerCode: PRVPQU databaseName: Science Database customDbUrl: eissn: 1548-7105 dateEnd: 20171231 omitProxy: false ssIdentifier: ssj0033425 issn: 1548-7091 databaseCode: M2P dateStart: 20041001 isFulltext: true titleUrlDefault: https://search.proquest.com/sciencejournals providerName: ProQuest
link	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1Za9wwEB6apIW-9D7cpFtBA6UPbmzJtrRPJQkJgZBl6QFLX4wtySTQ2sl6t9B_3xlJ3hwNeenLwOKxV2Y0hzzHB7DNG2W0xkMOhvpVnJkqQZ3jKraiSK00vFbCg03IyUTNZuNpqM3pQ1nlYBOdoTadpm_kO5xcv0Jv8_n8IibQKEquBgSNNdjAwCaliq4TPh0MsRCZw1yloDyW6BeH6aRC7bSEz4zmglqor_ijm1b5ilu6kSd17ufw8X8u_Ak8CnEn2_Ub5Sncs-0zeOCRKP88h9Oj7leHe8l2y575PIKdMz_mmVnXX9Wzi2XVup40tJCMHKL_jsiq1XRP1jVsKFFkC3KDg1FiAdjnBXw_PPi2fxQHEIZY40lxEWfKJAUNCTMF56lorGpsZQuakZOLJDF4uC5spepamLFouDA2F7LWRnNj8HCWipew3natfQ3MpE1dWXxIYjQB241NraTludFJ3qTKRPBxEEWpw4RyAsr4WbpMuVClE1tJYovg_Yr33M_luJXrA0m0JGXFJ-kq9BzgemjsVblLYAEUQ2YRbF3jRCXT1y5vDsItg5L35UqyEbxbXaUbqW7NCazkSU55yTs4JM1czCRyvPJb7fJ1qOsZI7YItoe9d_nf_77rmzuXuAkPfaULVc9twfpivrRv4b7-vTjr5yNYkzPpqBrBxt7BZPoFfx3vfUJ6khyPnF4RlZ5-RTrNf_wFPiksMQ
linkProvider	ProQuest
linkToHtml	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1V1Lb9QwEB6VAioX3oXQQi1RhDhETew8vAeEKqDaqmXVQ5F6M4ntCCRI2s0uqD-K_8iMHW8foN564BzHiePxfJ545vsANnkjjdYY5OBWv4ozUyW45riMrShSWxpeS-HFJsrJRB4djQ6W4HeohaG0yuATnaM2naZ_5FucoF8i2rw7PolJNIoOV4OChreKPXv6CyO2_u3uB5zeV5zvfDx8P44HUYFYY-QzizNpkoJIr0zBeSoaKxtb2YI4X3KRJAaDxcJWsq6FGYmGC2NzUdbaaG4MBhupwH5vwM2MiMUoU5AfBMcvROY0XikIiEvE4cCGKuRWS3rQ6J6oZPsc_l1GgXMweOlc1sHdzr3_7EPdh7vDvppt-4XwAJZs-xBue6XN00fwddz96HCt2G7eM39OYqfM01gz6-rHenYyr1pXc4cIwAjw_X9SVi3YS1nXsJCCyWYE88HpskG46DF8vpZhrsJy27X2KTCTNnVlsZPEaBLuG5lalpbnRid5k0oTwZsw9UoPDOwkBPJduUwAIZUzE0VmEsHLRdtjzzvyz1avyYIUOSPsSVdDTQW-D9F6qW0SQ6A9chbB-oWW6ET0hctrwZjU4MR6tbCkCDYWV-lGystzE6Z4ktO56xUtSuKUzEps8cSb9tlwqKobd6QRbAZbP3v232N9duUrbsDK-PDTvtrfneytwR2f1UOZguuwPJvO7XO4pX_OvvXTF27NMvhy3bb_B2zQgDI
linkToPdf	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1V1Lb9QwEB6VAhWX8oa0hVqiCPUQbWLn4T1UVUVZtSpa7QGkiotJbEcglaTd7IL60_h3zMTx9gHqrQfOmfXGyef5PPHMfABbvJJGawxycKtfhIkpIlxzXIZWZLHNDS-lcGIT-Xgsj4-HkyX47WthKK3S-8TOUZtG0zfyASfql8g2g6rPipjsj3ZPz0ISkKKDVq-m4RByZM9_YfTW7hzu46t-y_now6f3B2EvMBBqjIJmYSJNlFEDLJNxHovKysoWNqP-L6mIIoOBY2YLWZbCDEXFhbGpyEttNDcGA49Y4Lh34G6OISZlE07SL54EhEg6vVcKCMIcOdl3RhVyUJM2NLoqKt--xIXXGeESJV47o-2ob_TwP35oj2C132-zPbdAHsOSrZ_AfafAef4Uvh00PxpcQ7aZt8ydn9gpc-2tme3qylp2Ni_qrhYPmYHRRsB9P2XFoqspayrmUzPZjOjfO2PWCxo9g8-3Ms3nsFw3tX0JzMRVWVgcJDKaBP2GppS55anRUVrF0gSw7WGgdN-ZnQRCTlSXISCk6iCjCDIBvFnYnrp-JP-0ekdoUuSkcCRd9LUWeD_U7kvtkUgC7Z2TADauWKJz0Vcur3tgqd65tWqBqgA2F1fph5Sv170wxaOUzmNvsMip12SSo8ULB_OL6VC1N-5UA9jyuL_477_nunbjLW7CCkJefTwcH63DA5fsQwmEG7A8m87tK7inf86-t9PX3fJl8PW2of8HRqeJHg
openUrl	ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Homogeneous+reporter+system+enables+quantitative+functional+assessment+of+multiple+transcription+factors&rft.jtitle=Nature+methods&rft.au=Romanov%2C+Sergei&rft.au=Medvedev%2C+Alexander&rft.au=Gambarian%2C+Maria&rft.au=Poltoratskaya%2C+Natalia&rft.date=2008-03-01&rft.issn=1548-7105&rft.eissn=1548-7105&rft.volume=5&rft.issue=3&rft.spage=253&rft_id=info:doi/10.1038%2Fnmeth.1186&rft.externalDBID=NO_FULL_TEXT
thumbnail_l	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1548-7091&client=summon
thumbnail_m	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1548-7091&client=summon
thumbnail_s	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1548-7091&client=summon