Development of diagnostic microsphere-based immunoassays for Heartland virus
•IgM and IgG microsphere immunoassays for Heartland virus were developed.•Both assays demonstrated >95 % sensitivity, specificity and accuracy.•Anti-HRTV IgM and anti-HRTV IgG was detected in human serum.•The HRTV microsphere immunoassays are intended as first-line diagnostic tools. Background. H...
Uložené v:
| Vydané v: | Journal of clinical virology Ročník 134; s. 104693 |
|---|---|
| Hlavní autori: | , , , , , , , , , |
| Médium: | Journal Article |
| Jazyk: | English |
| Vydavateľské údaje: |
Netherlands
Elsevier B.V
01.01.2021
|
| Predmet: | |
| ISSN: | 1386-6532, 1873-5967, 1873-5967 |
| On-line prístup: | Získať plný text |
| Tagy: |
Pridať tag
Žiadne tagy, Buďte prvý, kto otaguje tento záznam!
|
| Abstract | •IgM and IgG microsphere immunoassays for Heartland virus were developed.•Both assays demonstrated >95 % sensitivity, specificity and accuracy.•Anti-HRTV IgM and anti-HRTV IgG was detected in human serum.•The HRTV microsphere immunoassays are intended as first-line diagnostic tools.
Background. Heartland virus (HRTV), a recently reclassified member of the genus Bandavirus, family Phenuiviridae, was first isolated in 2009 from a Missouri farmer exhibiting leukopenia and thrombocytopenia with suspected ehrlichiosis. Since then, more HRTV cases have been diagnosed, and firstline laboratory diagnostic assays are needed to identify future infections Objectives. We sought to develop rapid and reliable IgM and IgG microsphere immunoassays (MIAs) to test sera of patients suspected of having HRTV infection, and to distinguish between recent and past infections. Study design. Heartland virus antigen was captured by an anti-HRTV monoclonal antibody covalently bound to microspheres. Antibodies in human sera from confirmed HRTV-positive and negative cases were reacted with the microsphere complexes and detected using a BioPlex® 200 instrument. Assay cutoffs were determined by receiver operator characteristic analysis of the normalized test output values, equivocal zones for each assay were defined, and sensitivities, specificities, accuracies, and imprecision values were calculated. Results. Sensitivities, specificities and accuracies of the IgM and IgG MIAs were all >95 %. Both tests were precise within and between assay plates, and cross-reactivity with other arboviruses was not observed. Conclusions. HRTV IgM and IgG MIAs are accurate and rapid first-line methods to serologically identify recent and past HRTV infections. |
|---|---|
| AbstractList | •IgM and IgG microsphere immunoassays for Heartland virus were developed.•Both assays demonstrated >95 % sensitivity, specificity and accuracy.•Anti-HRTV IgM and anti-HRTV IgG was detected in human serum.•The HRTV microsphere immunoassays are intended as first-line diagnostic tools.
Background. Heartland virus (HRTV), a recently reclassified member of the genus Bandavirus, family Phenuiviridae, was first isolated in 2009 from a Missouri farmer exhibiting leukopenia and thrombocytopenia with suspected ehrlichiosis. Since then, more HRTV cases have been diagnosed, and firstline laboratory diagnostic assays are needed to identify future infections Objectives. We sought to develop rapid and reliable IgM and IgG microsphere immunoassays (MIAs) to test sera of patients suspected of having HRTV infection, and to distinguish between recent and past infections. Study design. Heartland virus antigen was captured by an anti-HRTV monoclonal antibody covalently bound to microspheres. Antibodies in human sera from confirmed HRTV-positive and negative cases were reacted with the microsphere complexes and detected using a BioPlex® 200 instrument. Assay cutoffs were determined by receiver operator characteristic analysis of the normalized test output values, equivocal zones for each assay were defined, and sensitivities, specificities, accuracies, and imprecision values were calculated. Results. Sensitivities, specificities and accuracies of the IgM and IgG MIAs were all >95 %. Both tests were precise within and between assay plates, and cross-reactivity with other arboviruses was not observed. Conclusions. HRTV IgM and IgG MIAs are accurate and rapid first-line methods to serologically identify recent and past HRTV infections. Heartland virus (HRTV), a recently reclassified member of the genus Bandavirus, family Phenuiviridae, was first isolated in 2009 from a Missouri farmer exhibiting leukopenia and thrombocytopenia with suspected ehrlichiosis. Since then, more HRTV cases have been diagnosed, and firstline laboratory diagnostic assays are needed to identify future infections Objectives. We sought to develop rapid and reliable IgM and IgG microsphere immunoassays (MIAs) to test sera of patients suspected of having HRTV infection, and to distinguish between recent and past infections. Heartland virus antigen was captured by an anti-HRTV monoclonal antibody covalently bound to microspheres. Antibodies in human sera from confirmed HRTV-positive and negative cases were reacted with the microsphere complexes and detected using a BioPlex® 200 instrument. Assay cutoffs were determined by receiver operator characteristic analysis of the normalized test output values, equivocal zones for each assay were defined, and sensitivities, specificities, accuracies, and imprecision values were calculated. Sensitivities, specificities and accuracies of the IgM and IgG MIAs were all >95 %. Both tests were precise within and between assay plates, and cross-reactivity with other arboviruses was not observed. HRTV IgM and IgG MIAs are accurate and rapid first-line methods to serologically identify recent and past HRTV infections. Heartland virus (HRTV), a recently reclassified member of the genus Bandavirus, family Phenuiviridae, was first isolated in 2009 from a Missouri farmer exhibiting leukopenia and thrombocytopenia with suspected ehrlichiosis. Since then, more HRTV cases have been diagnosed, and firstline laboratory diagnostic assays are needed to identify future infections Objectives. We sought to develop rapid and reliable IgM and IgG microsphere immunoassays (MIAs) to test sera of patients suspected of having HRTV infection, and to distinguish between recent and past infections.BACKGROUNDHeartland virus (HRTV), a recently reclassified member of the genus Bandavirus, family Phenuiviridae, was first isolated in 2009 from a Missouri farmer exhibiting leukopenia and thrombocytopenia with suspected ehrlichiosis. Since then, more HRTV cases have been diagnosed, and firstline laboratory diagnostic assays are needed to identify future infections Objectives. We sought to develop rapid and reliable IgM and IgG microsphere immunoassays (MIAs) to test sera of patients suspected of having HRTV infection, and to distinguish between recent and past infections.Heartland virus antigen was captured by an anti-HRTV monoclonal antibody covalently bound to microspheres. Antibodies in human sera from confirmed HRTV-positive and negative cases were reacted with the microsphere complexes and detected using a BioPlex® 200 instrument. Assay cutoffs were determined by receiver operator characteristic analysis of the normalized test output values, equivocal zones for each assay were defined, and sensitivities, specificities, accuracies, and imprecision values were calculated.STUDY DESIGNHeartland virus antigen was captured by an anti-HRTV monoclonal antibody covalently bound to microspheres. Antibodies in human sera from confirmed HRTV-positive and negative cases were reacted with the microsphere complexes and detected using a BioPlex® 200 instrument. Assay cutoffs were determined by receiver operator characteristic analysis of the normalized test output values, equivocal zones for each assay were defined, and sensitivities, specificities, accuracies, and imprecision values were calculated.Sensitivities, specificities and accuracies of the IgM and IgG MIAs were all >95 %. Both tests were precise within and between assay plates, and cross-reactivity with other arboviruses was not observed.RESULTSSensitivities, specificities and accuracies of the IgM and IgG MIAs were all >95 %. Both tests were precise within and between assay plates, and cross-reactivity with other arboviruses was not observed.HRTV IgM and IgG MIAs are accurate and rapid first-line methods to serologically identify recent and past HRTV infections.CONCLUSIONSHRTV IgM and IgG MIAs are accurate and rapid first-line methods to serologically identify recent and past HRTV infections. |
| ArticleNumber | 104693 |
| Author | Goodman, Christin H. Brault, Aaron C. Horiuchi, Kalanthe Panella, Amanda J. Staples, J. Erin Velez, Jason O. Pastula, Daniel M. Kosoy, Olga Calvert, Amanda E. Basile, Alison Jane |
| Author_xml | – sequence: 1 givenname: Alison Jane surname: Basile fullname: Basile, Alison Jane email: ajj1@cdc.gov – sequence: 2 givenname: Kalanthe surname: Horiuchi fullname: Horiuchi, Kalanthe – sequence: 3 givenname: Christin H. surname: Goodman fullname: Goodman, Christin H. – sequence: 4 givenname: Olga surname: Kosoy fullname: Kosoy, Olga – sequence: 5 givenname: Amanda J. surname: Panella fullname: Panella, Amanda J. – sequence: 6 givenname: Jason O. surname: Velez fullname: Velez, Jason O. – sequence: 7 givenname: Daniel M. surname: Pastula fullname: Pastula, Daniel M. – sequence: 8 givenname: Aaron C. surname: Brault fullname: Brault, Aaron C. – sequence: 9 givenname: J. Erin surname: Staples fullname: Staples, J. Erin – sequence: 10 givenname: Amanda E. surname: Calvert fullname: Calvert, Amanda E. |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/33248359$$D View this record in MEDLINE/PubMed |
| BookMark | eNqFkLtOwzAUQC0EglL4ABaUkSXFrziJmFB5FKkSC7vl2DfgktjFdir170lVYGCAybZ0ztX1OUWHzjtA6ILgGcFEXK9mK72ZUUx3by5qdoAmpCpZXtSiPBzvrBK5KBg9QacxrjAmBePlMTphjPKKFfUELe9gA51f9-BS5tvMWPXqfExWZ73Vwcf1GwTIGxXBZLbvB-dVjGobs9aHbAEqpE45k21sGOIZOmpVF-H865yil4f7l_kiXz4_Ps1vl7nmXKScYV1y4A1VhIkCuMFCCd2IWhtDKbQKV7ythSIVbouSNkrULRWN4UxgrA2boqv92HXwHwPEJHsbNXTjIuCHKCkXBedjAz6il1_o0PRg5DrYXoWt_A4wAmQP7P4aA7Q_CMFyF1mu5BhZ7iLLfeTRKX852iaVrHcpKNv9ad7sTRjrbCwEGbUFp8HYADpJ4-2fdv3L1p11VqvuHbb_uJ8pbamv |
| CitedBy_id | crossref_primary_10_1371_journal_pntd_0010156 crossref_primary_10_3390_microorganisms12020286 crossref_primary_10_1093_jme_tjad060 crossref_primary_10_1128_spectrum_01966_23 crossref_primary_10_1089_vim_2025_0019 |
| Cites_doi | 10.1128/CVI.00291-09 10.1128/jcm.4.6.503-510.1976 10.3201/eid2502.181288 10.1128/JCM.00182-09 10.4269/ajtmh.15-0473 10.4269/ajtmh.13-0209 10.1093/jme/tjy103 10.1016/j.jviromet.2014.07.030 10.1128/jvi.69.8.4792-4796.1995 10.1056/NEJMoa1203378 10.1093/ofid/ofaa125 10.1371/journal.pone.0075670 10.1093/jme/tjw080 |
| ContentType | Journal Article |
| Copyright | 2020 Published by Elsevier B.V. |
| Copyright_xml | – notice: 2020 – notice: Published by Elsevier B.V. |
| DBID | 6I. AAFTH AAYXX CITATION NPM 7X8 |
| DOI | 10.1016/j.jcv.2020.104693 |
| DatabaseName | ScienceDirect Open Access Titles Elsevier:ScienceDirect:Open Access CrossRef PubMed MEDLINE - Academic |
| DatabaseTitle | CrossRef PubMed MEDLINE - Academic |
| DatabaseTitleList | PubMed MEDLINE - Academic |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: 7X8 name: MEDLINE - Academic url: https://search.proquest.com/medline sourceTypes: Aggregation Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Biology |
| EISSN | 1873-5967 |
| ExternalDocumentID | 33248359 10_1016_j_jcv_2020_104693 S1386653220304352 |
| Genre | Journal Article |
| GroupedDBID | --- --K --M .1- .FO .GJ .~1 0R~ 1B1 1P~ 1RT 1~. 1~5 29K 4.4 457 4CK 4G. 53G 5GY 5VS 7-5 71M 8P~ AAAJQ AABNK AAEDT AAEDW AAIKJ AAKOC AALRI AAOAW AAQFI AAQXK AARKO AATTM AAXKI AAXUO AAYWO ABBQC ABFNM ABFRF ABJNI ABMAC ABMZM ABWVN ABXDB ACDAQ ACGFO ACGFS ACIEU ACIUM ACLOT ACRLP ACRPL ACVFH ADBBV ADCNI ADEZE ADMUD ADNMO ADVLN AEBSH AEFWE AEIPS AEKER AENEX AEUPX AEVXI AFJKZ AFPUW AFRHN AFTJW AFXIZ AGEKW AGHFR AGQPQ AGUBO AGYEJ AIEXJ AIGII AIIUN AIKHN AITUG AJRQY AJUYK AKBMS AKRWK AKYEP ALMA_UNASSIGNED_HOLDINGS AMRAJ ANKPU ANZVX APXCP ASPBG AVWKF AXJTR AZFZN BKOJK BLXMC BNPGV CJTIS CS3 D-I DU5 EBS EFJIC EFKBS EFLBG EJD EO8 EO9 EP2 EP3 F5P FDB FEDTE FGOYB FIRID FNPLU FYGXN G-Q GBLVA HVGLF HX~ HZ~ IHE J1W KOM LUGTX M41 MO0 N9A O-L O9- OAUVE OD- OO. OZT P-8 P-9 P2P PC. Q38 R2- ROL RPZ SCC SDF SDG SDP SEL SES SEW SPCBC SSH SSI SSZ T5K UNMZH Z5R ~G- ~HD 6I. AACTN AAFTH AAIAV ABLVK ABYKQ AFCTW AFKWA AJBFU AJOXV AMFUW LCYCR RIG 9DU AAYXX CITATION NPM 7X8 |
| ID | FETCH-LOGICAL-c446t-30c74e4b2a1365e4d06a6cb69cdd22efa084f96a180f572ba69f26bd43600cd3 |
| ISICitedReferencesCount | 6 |
| ISICitedReferencesURI | http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000609162400007&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| ISSN | 1386-6532 1873-5967 |
| IngestDate | Sat Sep 27 22:37:10 EDT 2025 Wed Feb 19 02:29:52 EST 2025 Tue Nov 18 22:11:54 EST 2025 Sat Nov 29 07:03:18 EST 2025 Fri Feb 23 02:48:34 EST 2024 Tue Oct 14 19:29:31 EDT 2025 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Keywords | Heartland virus Immunoglobulin M Microsphere immunoassay Diagnosis MIA Immunoglobulin G |
| Language | English |
| License | This is an open access article under the CC BY-NC-ND license. Published by Elsevier B.V. |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-c446t-30c74e4b2a1365e4d06a6cb69cdd22efa084f96a180f572ba69f26bd43600cd3 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| OpenAccessLink | https://dx.doi.org/10.1016/j.jcv.2020.104693 |
| PMID | 33248359 |
| PQID | 2465445964 |
| PQPubID | 23479 |
| ParticipantIDs | proquest_miscellaneous_2465445964 pubmed_primary_33248359 crossref_primary_10_1016_j_jcv_2020_104693 crossref_citationtrail_10_1016_j_jcv_2020_104693 elsevier_sciencedirect_doi_10_1016_j_jcv_2020_104693 elsevier_clinicalkey_doi_10_1016_j_jcv_2020_104693 |
| PublicationCentury | 2000 |
| PublicationDate | January 2021 2021-01-00 2021-Jan 20210101 |
| PublicationDateYYYYMMDD | 2021-01-01 |
| PublicationDate_xml | – month: 01 year: 2021 text: January 2021 |
| PublicationDecade | 2020 |
| PublicationPlace | Netherlands |
| PublicationPlace_xml | – name: Netherlands |
| PublicationTitle | Journal of clinical virology |
| PublicationTitleAlternate | J Clin Virol |
| PublicationYear | 2021 |
| Publisher | Elsevier B.V |
| Publisher_xml | – name: Elsevier B.V |
| References | Lindsey, Calisher, Mathews (bib0030) 1976; 4 Brault, Savage, Duggal, Eisen, Staples (bib0035) 2018 Basile (bib0075) 2010 Lambert, Lanciotti (bib0025) 2009; 47 Davison, Hinkley (bib0085) 1997 Basile, Horiuchi, Panella, Laven, Kosoy, Lanciotti (bib0095) 2013; 8 Team (bib0080) 2012 Goodman, Russell, Velez, Laven, Nicholson, Bagarozzi (bib0070) 2014; 208 Pastula, Turabelidze, Yates, Jones, Lambert, Panella (bib0040) 2014; 63 Godsey, Savage, Burkhalter, Bosco-Lauth, Delorey (bib0010) 2016; 53 Storch (bib0050) 2007 Johnson, Noga, Kosoy, Lanciotti, Johnson, Biggerstaff (bib0065) 2005; 12 Basile, Biggerstaff, Kosoy, Junna, Panella, Powers (bib0100) 2010; 17 Staples, Pastula, Panella, Rabe, Kosoy, Walker, Velez, Lambert, Fischer (bib0045) 2020; 7 Calvert, Brault (bib0060) 2015; 93 van den Broek, Müller, Huang, Aguet, Zinkernagel (bib0055) 1995; 69 Savage, Godsey, Tatman, Burkhalter, Hamm, Panella (bib0020) 2018; 55 Savage, Godsey, Lambert, Panella, Burkhalter, Harmon (bib0015) 2013; 89 McMullan, Folk, Kelly, MacNeil, Goldsmith, Metcalfe (bib0005) 2012; 367 Lindsey, Menitove, Biggerstaff, Turabelidze, Parton, Peck (bib0090) 2019; 25 Lambert (10.1016/j.jcv.2020.104693_bib0025) 2009; 47 Calvert (10.1016/j.jcv.2020.104693_bib0060) 2015; 93 Pastula (10.1016/j.jcv.2020.104693_bib0040) 2014; 63 Team (10.1016/j.jcv.2020.104693_bib0080) 2012 Staples (10.1016/j.jcv.2020.104693_bib0045) 2020; 7 Davison (10.1016/j.jcv.2020.104693_bib0085) 1997 Basile (10.1016/j.jcv.2020.104693_bib0095) 2013; 8 Godsey (10.1016/j.jcv.2020.104693_bib0010) 2016; 53 Basile (10.1016/j.jcv.2020.104693_bib0075) 2010 Lindsey (10.1016/j.jcv.2020.104693_bib0030) 1976; 4 Basile (10.1016/j.jcv.2020.104693_bib0100) 2010; 17 McMullan (10.1016/j.jcv.2020.104693_bib0005) 2012; 367 Savage (10.1016/j.jcv.2020.104693_bib0015) 2013; 89 Brault (10.1016/j.jcv.2020.104693_bib0035) 2018 Johnson (10.1016/j.jcv.2020.104693_bib0065) 2005; 12 Storch (10.1016/j.jcv.2020.104693_bib0050) 2007 Goodman (10.1016/j.jcv.2020.104693_bib0070) 2014; 208 Lindsey (10.1016/j.jcv.2020.104693_bib0090) 2019; 25 Savage (10.1016/j.jcv.2020.104693_bib0020) 2018; 55 van den Broek (10.1016/j.jcv.2020.104693_bib0055) 1995; 69 |
| References_xml | – volume: 47 start-page: 2398 year: 2009 end-page: 2404 ident: bib0025 article-title: Consensus amplification and novel multiplex sequencing method for S segment species identification of 47 viruses of the Orthobunyavirus, Phlebovirus, and Nairovirus genera of the family Bunyaviridae publication-title: J. Clin. Microbiol. – start-page: 10 year: 2018 ident: bib0035 article-title: Heartland Virus Epidemiology – volume: 69 start-page: 4792 year: 1995 end-page: 4796 ident: bib0055 article-title: Antiviral defense in mice lacking both alpha/beta and gamma interferon receptors publication-title: J. Virol. – volume: 208 start-page: 66 year: 2014 end-page: 78 ident: bib0070 article-title: Development of an algorithm for production of inactivated arbovirus antigens in cell culture publication-title: J. Virol. Methods – volume: 367 start-page: 834 year: 2012 end-page: 841 ident: bib0005 article-title: A new phlebovirus associated with severe febrile illness in Missouri publication-title: N. Engl. J. Med. – volume: 89 start-page: 445 year: 2013 end-page: 452 ident: bib0015 article-title: First detection of heartland virus (Bunyaviridae: Phlebovirus) from field collected arthropods publication-title: Am. J. Trop. Med. Hyg. – volume: 93 start-page: 1338 year: 2015 end-page: 1340 ident: bib0060 article-title: Development and characterization of monoclonal antibodies directed against the nucleoprotein of heartland virus publication-title: Am. J. Trop. Med. Hyg. – volume: 17 start-page: 56 year: 2010 end-page: 61 ident: bib0100 article-title: Removal of species constraints in antibody detection publication-title: Clin. Vaccine Immunol. – year: 2010 ident: bib0075 article-title: Stability of reagents associated with microsphere immunoassays over time using different storage solutions publication-title: American Society of Tropical Medicine and Hygiene Annual Meeting. Atlanta, GA – volume: 7 start-page: 1 year: 2020 end-page: 7 ident: bib0045 article-title: Investigation of Heartland virus disease throughout the United States, 2013-2017 publication-title: Open Forum Infect. Dis. – volume: 53 start-page: 1226 year: 2016 end-page: 1233 ident: bib0010 article-title: Transmission of heartland virus (Bunyaviridae: Phlebovirus) by experimentally infected amblyomma americanum (Acari: Ixodidae) publication-title: J. Med. Entomol. – volume: 8 year: 2013 ident: bib0095 article-title: Multiplex microsphere immunoassays for the detection of IgM and IgG to arboviral diseases publication-title: PLoS One – volume: 4 start-page: 503 year: 1976 end-page: 510 ident: bib0030 article-title: Serum dilution neutralization test for California group virus identification and serology publication-title: J. Clin. Microbiol. – year: 2012 ident: bib0080 article-title: R: A Language and Environment for Statisticalcomputing – volume: 63 start-page: 270 year: 2014 end-page: 271 ident: bib0040 article-title: Notes from the field: heartland virus disease – United States, 2012-2013 publication-title: MMWR Morb. Mortal. Wkly. Rep. – year: 1997 ident: bib0085 article-title: Bootstrap Methods and Their Applications – volume: 12 start-page: 566 year: 2005 end-page: 574 ident: bib0065 article-title: Duplex microsphere-based immunoassay for detection of anti-West Nile virus and anti-St. Louis encephalitis virus immunoglobulin m antibodies publication-title: Clin. Diagn. Lab. Immunol. – volume: 25 start-page: 358 year: 2019 end-page: 360 ident: bib0090 article-title: Seroprevalence of heartland virus antibodies in blood donors, Northwestern Missouri, USA publication-title: Emerging Infect. Dis. – volume: 55 start-page: 1613 year: 2018 end-page: 1616 ident: bib0020 article-title: Surveillance for heartland and bourbon viruses in Eastern Kansas, June 2016 publication-title: J. Med. Entomol. – year: 2007 ident: bib0050 article-title: Diagnostic Virology – year: 2010 ident: 10.1016/j.jcv.2020.104693_bib0075 article-title: Stability of reagents associated with microsphere immunoassays over time using different storage solutions publication-title: American Society of Tropical Medicine and Hygiene Annual Meeting. Atlanta, GA – volume: 17 start-page: 56 year: 2010 ident: 10.1016/j.jcv.2020.104693_bib0100 article-title: Removal of species constraints in antibody detection publication-title: Clin. Vaccine Immunol. doi: 10.1128/CVI.00291-09 – volume: 4 start-page: 503 year: 1976 ident: 10.1016/j.jcv.2020.104693_bib0030 article-title: Serum dilution neutralization test for California group virus identification and serology publication-title: J. Clin. Microbiol. doi: 10.1128/jcm.4.6.503-510.1976 – volume: 25 start-page: 358 year: 2019 ident: 10.1016/j.jcv.2020.104693_bib0090 article-title: Seroprevalence of heartland virus antibodies in blood donors, Northwestern Missouri, USA publication-title: Emerging Infect. Dis. doi: 10.3201/eid2502.181288 – volume: 63 start-page: 270 year: 2014 ident: 10.1016/j.jcv.2020.104693_bib0040 article-title: Notes from the field: heartland virus disease – United States, 2012-2013 publication-title: MMWR Morb. Mortal. Wkly. Rep. – volume: 47 start-page: 2398 year: 2009 ident: 10.1016/j.jcv.2020.104693_bib0025 article-title: Consensus amplification and novel multiplex sequencing method for S segment species identification of 47 viruses of the Orthobunyavirus, Phlebovirus, and Nairovirus genera of the family Bunyaviridae publication-title: J. Clin. Microbiol. doi: 10.1128/JCM.00182-09 – volume: 93 start-page: 1338 year: 2015 ident: 10.1016/j.jcv.2020.104693_bib0060 article-title: Development and characterization of monoclonal antibodies directed against the nucleoprotein of heartland virus publication-title: Am. J. Trop. Med. Hyg. doi: 10.4269/ajtmh.15-0473 – volume: 89 start-page: 445 year: 2013 ident: 10.1016/j.jcv.2020.104693_bib0015 article-title: First detection of heartland virus (Bunyaviridae: Phlebovirus) from field collected arthropods publication-title: Am. J. Trop. Med. Hyg. doi: 10.4269/ajtmh.13-0209 – volume: 55 start-page: 1613 year: 2018 ident: 10.1016/j.jcv.2020.104693_bib0020 article-title: Surveillance for heartland and bourbon viruses in Eastern Kansas, June 2016 publication-title: J. Med. Entomol. doi: 10.1093/jme/tjy103 – year: 2007 ident: 10.1016/j.jcv.2020.104693_bib0050 – volume: 208 start-page: 66 year: 2014 ident: 10.1016/j.jcv.2020.104693_bib0070 article-title: Development of an algorithm for production of inactivated arbovirus antigens in cell culture publication-title: J. Virol. Methods doi: 10.1016/j.jviromet.2014.07.030 – start-page: 10 year: 2018 ident: 10.1016/j.jcv.2020.104693_bib0035 – volume: 69 start-page: 4792 year: 1995 ident: 10.1016/j.jcv.2020.104693_bib0055 article-title: Antiviral defense in mice lacking both alpha/beta and gamma interferon receptors publication-title: J. Virol. doi: 10.1128/jvi.69.8.4792-4796.1995 – volume: 12 start-page: 566 year: 2005 ident: 10.1016/j.jcv.2020.104693_bib0065 article-title: Duplex microsphere-based immunoassay for detection of anti-West Nile virus and anti-St. Louis encephalitis virus immunoglobulin m antibodies publication-title: Clin. Diagn. Lab. Immunol. – year: 1997 ident: 10.1016/j.jcv.2020.104693_bib0085 – volume: 367 start-page: 834 year: 2012 ident: 10.1016/j.jcv.2020.104693_bib0005 article-title: A new phlebovirus associated with severe febrile illness in Missouri publication-title: N. Engl. J. Med. doi: 10.1056/NEJMoa1203378 – volume: 7 start-page: 1 issue: 5 year: 2020 ident: 10.1016/j.jcv.2020.104693_bib0045 article-title: Investigation of Heartland virus disease throughout the United States, 2013-2017 publication-title: Open Forum Infect. Dis. doi: 10.1093/ofid/ofaa125 – year: 2012 ident: 10.1016/j.jcv.2020.104693_bib0080 – volume: 8 year: 2013 ident: 10.1016/j.jcv.2020.104693_bib0095 article-title: Multiplex microsphere immunoassays for the detection of IgM and IgG to arboviral diseases publication-title: PLoS One doi: 10.1371/journal.pone.0075670 – volume: 53 start-page: 1226 year: 2016 ident: 10.1016/j.jcv.2020.104693_bib0010 article-title: Transmission of heartland virus (Bunyaviridae: Phlebovirus) by experimentally infected amblyomma americanum (Acari: Ixodidae) publication-title: J. Med. Entomol. doi: 10.1093/jme/tjw080 |
| SSID | ssj0015347 |
| Score | 2.3367743 |
| Snippet | •IgM and IgG microsphere immunoassays for Heartland virus were developed.•Both assays demonstrated >95 % sensitivity, specificity and accuracy.•Anti-HRTV IgM... Heartland virus (HRTV), a recently reclassified member of the genus Bandavirus, family Phenuiviridae, was first isolated in 2009 from a Missouri farmer... |
| SourceID | proquest pubmed crossref elsevier |
| SourceType | Aggregation Database Index Database Enrichment Source Publisher |
| StartPage | 104693 |
| SubjectTerms | Diagnosis Heartland virus Immunoglobulin G Immunoglobulin M MIA Microsphere immunoassay |
| Title | Development of diagnostic microsphere-based immunoassays for Heartland virus |
| URI | https://www.clinicalkey.com/#!/content/1-s2.0-S1386653220304352 https://dx.doi.org/10.1016/j.jcv.2020.104693 https://www.ncbi.nlm.nih.gov/pubmed/33248359 https://www.proquest.com/docview/2465445964 |
| Volume | 134 |
| WOSCitedRecordID | wos000609162400007&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| journalDatabaseRights | – providerCode: PRVESC databaseName: Elsevier SD Freedom Collection Journals 2021 customDbUrl: eissn: 1873-5967 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0015347 issn: 1386-6532 databaseCode: AIEXJ dateStart: 20161001 isFulltext: true titleUrlDefault: https://www.sciencedirect.com providerName: Elsevier |
| link | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV3db5swELfSdpP2Mu172UfFpD2togLbGPyYTZ26qur2EE15Q8bGE1EKFaRRu79-ZwwObZRufdgLQsg2hjufz3e_u0PoY0gyrSKK_Uxlwqcq437CMfNpKJnOtaayhfz_PI3PzpLZjP8YjX73sTCrRVyWydUVv_ivpIZnQGwTOnsPcrtB4QHcA9HhCmSH6z8RfgADMpqgslg6k5f13IDvGpNHIPfN7qUOChMdUoH-LK7bvAwmKqleGrDjwaqoO5vApurqwilNkNzQLP9ZNIXFJ08WbW3DE7F22x9XdWFKr1gQB7wEdE8H_6kq1dlibboDk4Lr0O0GVWNNht8Xv8TQToHDgZ3CilaSMJ9F5Kbs7SyZVnoaf7Otl7gh2K2NYX44lys41GPrm7ZtbybRvrW5Ochhj2abpzBEaoZI7RA7aA_HEQeJuDf5djQ7cT6oiLTl6dy8e594iw68NY9tWs22U0urvUyfoMcd7byJZZenaJSXz9BDW4j0-jk6HTCNV2lvzTTeBtN4Q6bxgGk8xzReyzQv0PTr0fTLsd_V2fAlpWzpk0DGNKcZFgbzmFMVMMFkxrhUCuNciyChmjMRJoGOYpwJxjVmmaIEtGWpyEu0W1Zl_hp5gSYmH6PCnGVU5iGnRBtHuyKRijNNxijof1Mquxz0phTKIt1KnjH65Lpc2AQsdzXG_b9P-6UAe2EKXHRXJ-o6dWqnVSf_1u1DT9wURLLxs8GSqi6bFJschTTijI7RK0t1N3UCBxg49PA39_mst-jRekW9Q7vL-jJ_jx7I1bJo6n20E8-S_Y57_wDCdbjP |
| linkProvider | Elsevier |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Development+of+diagnostic+microsphere-based+immunoassays+for+Heartland+virus&rft.jtitle=Journal+of+clinical+virology&rft.au=Basile%2C+Alison+Jane&rft.au=Horiuchi%2C+Kalanthe&rft.au=Goodman%2C+Christin+H.&rft.au=Kosoy%2C+Olga&rft.date=2021-01-01&rft.issn=1386-6532&rft.volume=134&rft.spage=104693&rft_id=info:doi/10.1016%2Fj.jcv.2020.104693&rft.externalDBID=n%2Fa&rft.externalDocID=10_1016_j_jcv_2020_104693 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1386-6532&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1386-6532&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1386-6532&client=summon |