The most abundant nascent poly(A)+ RNAs are transcribed by RNA polymerase III in murine tumor cells

Twelve to twenty percent of newly synthesized poly(A) + RNA is transcribed by RNA polymerase III in Ehrlich ascites carcinoma and P3O1 plasmocytoma mouse tumors. Most of this RNA designated as pol IIIpoly(A) + RNA has a size of 160 to 800 nucleotides with a maximum of distribution of ca. 300 nucleot...

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Veröffentlicht in:Nucleic acids research Jg. 18; H. 15; S. 4499 - 4506
Hauptverfasser: Kramerov, D.A., Tillib, S.V., Shumyatsky, G.P., Georgiev, G.P.
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Oxford Oxford University Press 11.08.1990
Schlagworte:
Animals
Biological and medical sciences
Carcinoma, Ehrlich Tumor
DNA-Directed RNA Polymerases - metabolism
Electrophoresis, Agar Gel
Electrophoresis, Polyacrylamide Gel
Fundamental and applied biological sciences. Psychology
Mice
Molecular and cellular biology
Molecular genetics
Molecular Weight
Nucleotide Mapping
Poly A - genetics
Poly A - metabolism
RNA - genetics
RNA - metabolism
RNA Polymerase III - metabolism
RNA, Messenger
Sequence Homology, Nucleic Acid
Transcription, Genetic
Transcription. Transcription factor. Splicing. Rna processing
Tumor Cells, Cultured
Ultracentrifugation
Transcription
Enzyme
Rodentia
Ultracentrifugation
RNA polymerase
Ehrlich ascite cell
Vertebrata
Sucrose gradient
Mammalia
Messenger RNA
Mouse
Polyadenylation
Tumor
Embryonal carcinoma
ISSN:0305-1048, 1362-4962
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Zusammenfassung:Twelve to twenty percent of newly synthesized poly(A) + RNA is transcribed by RNA polymerase III in Ehrlich ascites carcinoma and P3O1 plasmocytoma mouse tumors. Most of this RNA designated as pol IIIpoly(A) + RNA has a size of 160 to 800 nucleotides with a maximum of distribution of ca. 300 nucleotides. Pol IIIpoly(A) + RNA fraction consists of two major classes of molecules corresponding to previously described B1 RNA and B2 RNA with the ratio of 1:4 to 2:3. All B2 RNAs present in poly(A) + fraction contain a long poly(A) segments at the 3' ends. Thus, RNA polymerase III transcripts can be polyadenylated. Several transcripts that hybridize with B2 probe were also observed in poly(A)- RNA. The major components consist of 180, 160, 120 and 95 nucleotides. The 180-nucleotide B2 RNA seems to be a primary transcript from B2 repeat. We suggest that other B2 RNAs are transcribed from truncated copies of B2 element.
Bibliographie:ObjectType-Article-1
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content type line 23
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/18.15.4499