The most abundant nascent poly(A)+ RNAs are transcribed by RNA polymerase III in murine tumor cells

Twelve to twenty percent of newly synthesized poly(A) + RNA is transcribed by RNA polymerase III in Ehrlich ascites carcinoma and P3O1 plasmocytoma mouse tumors. Most of this RNA designated as pol IIIpoly(A) + RNA has a size of 160 to 800 nucleotides with a maximum of distribution of ca. 300 nucleot...

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Vydáno v:	Nucleic acids research Ročník 18; číslo 15; s. 4499 - 4506
Hlavní autoři:	Kramerov, D.A., Tillib, S.V., Shumyatsky, G.P., Georgiev, G.P.
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	Oxford Oxford University Press 11.08.1990
Témata:	Animals Biological and medical sciences Carcinoma, Ehrlich Tumor DNA-Directed RNA Polymerases - metabolism Electrophoresis, Agar Gel Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology Mice Molecular and cellular biology Molecular genetics Molecular Weight Nucleotide Mapping Poly A - genetics Poly A - metabolism RNA - genetics RNA - metabolism RNA Polymerase III - metabolism RNA, Messenger Sequence Homology, Nucleic Acid Transcription, Genetic Transcription. Transcription factor. Splicing. Rna processing Tumor Cells, Cultured Ultracentrifugation Transcription Enzyme Rodentia Ultracentrifugation RNA polymerase Ehrlich ascite cell Vertebrata Sucrose gradient Mammalia Messenger RNA Mouse Polyadenylation Tumor Embryonal carcinoma
ISSN:	0305-1048, 1362-4962
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Popis
Shrnutí:	Twelve to twenty percent of newly synthesized poly(A) + RNA is transcribed by RNA polymerase III in Ehrlich ascites carcinoma and P3O1 plasmocytoma mouse tumors. Most of this RNA designated as pol IIIpoly(A) + RNA has a size of 160 to 800 nucleotides with a maximum of distribution of ca. 300 nucleotides. Pol IIIpoly(A) + RNA fraction consists of two major classes of molecules corresponding to previously described B1 RNA and B2 RNA with the ratio of 1:4 to 2:3. All B2 RNAs present in poly(A) + fraction contain a long poly(A) segments at the 3' ends. Thus, RNA polymerase III transcripts can be polyadenylated. Several transcripts that hybridize with B2 probe were also observed in poly(A)- RNA. The major components consist of 180, 160, 120 and 95 nucleotides. The 180-nucleotide B2 RNA seems to be a primary transcript from B2 repeat. We suggest that other B2 RNAs are transcribed from truncated copies of B2 element.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0305-1048 1362-4962
DOI:	10.1093/nar/18.15.4499