Normalization and statistical analysis of multiplexed bead-based immunoassay data using mixed-effects modeling

Multiplexed bead-based flow cytometric immunoassays are a powerful experimental tool for investigating cellular communication networks, yet their widespread adoption is limited in part by challenges in robust quantitative analysis of the measurements. Here we report our application of mixed-effects...

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Vydané v:	Molecular & cellular proteomics Ročník 12; číslo 1; s. 245
Hlavní autori:	Clarke, David C, Morris, Melody K, Lauffenburger, Douglas A
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	United States 01.01.2013
Predmet:	Cell Line Flow Cytometry - methods Hep G2 Cells Humans Immunoassay - methods Interleukin-1alpha - metabolism Interleukin-6 - metabolism JNK Mitogen-Activated Protein Kinases - metabolism MAP Kinase Signaling System Models, Molecular p38 Mitogen-Activated Protein Kinases - metabolism Phosphoproteins - analysis Phosphorylation Protein Processing, Post-Translational Proteomics - methods Transforming Growth Factor alpha - metabolism Tumor Necrosis Factor-alpha - metabolism
ISSN:	1535-9484, 1535-9484
On-line prístup:	Zistit podrobnosti o prístupe
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