MALDI imaging mass spectrometry: molecular snapshots of biochemical systems

Matrix-assisted laser desorption/ionization ( MALDI ) imaging mass spectrometry (IMS) is emerging as a powerful tool for investigating the distribution of molecules within biological systems through the direct analysis of thin tissue sections. Unique among imaging methods, MALDI -IMS can determine t...

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Bibliographic Details
Published in:Nature methods Vol. 4; no. 10; pp. 828 - 833
Main Authors: Cornett, Dale S, Reyzer, Michelle L, Chaurand, Pierre, Caprioli, Richard M
Format: Journal Article
Language:English
Published: New York Nature Publishing Group US 01.10.2007
Nature Publishing Group
Subjects:
Animals
Biochemistry
Bioinformatics
Biological Microscopy
Biological systems
Biological Techniques
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
Desorption
Developmental Biology - methods
Ionization
Lasers
Life Sciences
Mass spectrometry
Medical examination
Methods
Molecular biology
Pathology - methods
perspective
Pharmaceutical Preparations - metabolism
Phospholipids - metabolism
Proteomics
Proteomics - methods
Proteomics - trends
Research methodology
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - trends
Technological change
Tissue microarrays
United States
ISSN:1548-7091, 1548-7105
Online Access:Get full text
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Summary:Matrix-assisted laser desorption/ionization ( MALDI ) imaging mass spectrometry (IMS) is emerging as a powerful tool for investigating the distribution of molecules within biological systems through the direct analysis of thin tissue sections. Unique among imaging methods, MALDI -IMS can determine the distribution of hundreds of unknown compounds in a single measurement. We discuss the current state of the art of MALDI -IMS along with some recent applications and technological developments that illustrate not only its current capabilities but also the future potential of the technique to provide a better understanding of the underlying molecular mechanisms of biological processes.
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ISSN:1548-7091
1548-7105
DOI:10.1038/nmeth1094