Expression of programmed cell death protein 1 and T‐cell immunoglobulin‐ and mucin‐domain‐containing molecule‐3 on peripheral blood CD4+CD8+ double positive T cells in patients with chronic hepatitis C virus infection and in subjects who spontaneously cleared the virus

Chronic hepatitis C virus (HCV) infection is characterized by increased proportion of CD4+CD8+ double positive (DP) T cells, but their role in this infection is unclear. In chronic hepatitis C, immune responses to HCV become functionally exhausted, which manifests itself by increased expression of p...

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Vydáno v:	Journal of viral hepatitis Ročník 26; číslo 8; s. 942 - 950
Hlavní autoři:	Caraballo Cortés, Kamila, Osuch, Sylwia, Perlejewski, Karol, Pawełczyk, Agnieszka, Kaźmierczak, Justyna, Janiak, Maciej, Jabłońska, Joanna, Nazzal, Khalil, Stelmaszczyk‐Emmel, Anna, Berak, Hanna, Bukowska‐Ośko, Iwona, Paciorek, Marcin, Laskus, Tomasz, Radkowski, Marek
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	England Wiley Subscription Services, Inc 01.08.2019 John Wiley and Sons Inc
Témata:	Adult Aged Apoptosis CD3 antigen CD4 antigen CD4-Positive T-Lymphocytes - metabolism CD8 antigen CD8-Positive T-Lymphocytes - metabolism Cell death Chronic infection DP T cells Epitopes Female Hepatitis Hepatitis A Virus Cellular Receptor 2 - metabolism Hepatitis C hepatitis C virus Hepatitis C, Chronic - blood Hepatitis C, Chronic - immunology Histocompatibility antigen HLA Humans Immunoglobulins Infections Interferon Leukocytes (mononuclear) Leukocytes, Mononuclear - metabolism Lymphocytes Lymphocytes T Major histocompatibility complex Male Middle Aged Mucin Original PD‐1 Peripheral blood mononuclear cells Phenotypes Programmed Cell Death 1 Receptor - metabolism T-Lymphocyte Subsets - metabolism Tim‐3 hepatitis C virus DP T cells PD-1 Tim-3
ISSN:	1352-0504, 1365-2893, 1365-2893
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Shrnutí:	Chronic hepatitis C virus (HCV) infection is characterized by increased proportion of CD4+CD8+ double positive (DP) T cells, but their role in this infection is unclear. In chronic hepatitis C, immune responses to HCV become functionally exhausted, which manifests itself by increased expression of programmed cell death protein 1 (PD‐1) and T‐cell immunoglobulin‐ and mucin‐domain‐containing molecule‐3 (Tim‐3) on T cells. The aim of our study was to determine PD‐1 and Tim‐3 phenotype of DP T cells in subjects with naturally resolved and chronic HCV infection. Peripheral blood mononuclear cells from 16 patients with chronic infection and 14 subjects who cleared HCV in the past were stained with anti‐CD3, anti‐CD4, anti‐CD8, anti‐PD‐1 and anti‐Tim‐3 antibodies and, in 12 HLA‐A*02‐positive subjects, MHC class I pentamer with HCV NS31406 epitope. In chronic and past HCV infection, proportions of total DP T cells and PD‐1+ DP T cells were similar but significantly higher than in healthy controls. DP T cells were more likely to be PD‐1+ than either CD4+ or CD8+ single positive (SP) T cells. HCV‐specific cells were present in higher proportions among DP T cells than among CD8+ SP T cells in both patient groups. Furthermore, while the majority of HCV‐specific DP T cells were PD‐1+, the proportion of HCV‐specific CD8+ T cells which were PD‐1+ was 4.9 and 1.9 times lower (chronic and past infection, respectively). PD‐1 and Tim‐3 were predominantly expressed on CD4highCD8low and CD4lowCD8high cells, respectively, and co‐expression of both markers was uncommon.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 ObjectType-Article-2 ObjectType-Feature-1 content type line 23
ISSN:	1352-0504 1365-2893 1365-2893
DOI:	10.1111/jvh.13108