Marker‐fusion PCR for one‐step mutagenesis of essential genes in yeast

We describe a one‐step gene replacement method based on fusion PCR that can be used to mutagenize essential genes at their endogenous locus. Marker‐fusion PCR can facilitate transfer of alleles between strains as well as PCR‐based techniques, such as site‐directed and error‐prone PCR mutagenesis, al...

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Vydané v:	Yeast (Chichester, England) Ročník 19; číslo 2; s. 141 - 149
Hlavní autori:	Kitazono, Ana A., Tobe, Brian T. D., Kalton, Helen, Diamant, Noam, Kron, Stephen J.
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	Chichester, UK John Wiley & Sons, Ltd 30.01.2002
Predmet:	Cdc28 CDC28 gene fusion‐PCR gene replacement Genes, Fungal integration mutagenesis Mutagenesis, Site-Directed Polymerase Chain Reaction - methods Saccharomyces cerevisiae screening Yeasts - genetics
ISSN:	0749-503X, 1097-0061
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