Adipose tissue‐derived extracellular matrix hydrogels as a release platform for secreted paracrine factors

Fat grafting is an established clinical intervention to promote tissue repair. The role of the fat's extracellular matrix (ECM) in regeneration is largely neglected. We investigated in vitro the use of human adipose tissue‐derived ECM hydrogels as release platform for factors secreted by adipos...

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Vydáno v:Journal of tissue engineering and regenerative medicine Ročník 13; číslo 6; s. 973 - 985
Hlavní autoři: Dongen, Joris A., Getova, Vasilena, Brouwer, Linda A., Liguori, Gabriel R., Sharma, Prashant K., Stevens, Hieronymus P., Lei, Berend, Harmsen, Martin C.
Médium: Journal Article
Jazyk:angličtina
Vydáno: England John Wiley & Sons, Inc 01.06.2019
John Wiley and Sons Inc
Témata:
acellular matrix
Adipose tissue
Adipose Tissue - metabolism
Angiogenesis
Animals
Cattle
Cell Proliferation - drug effects
Culture Media, Conditioned - pharmacology
decellularization
Deoxyribonucleic acid
Diabetes
Diabetes mellitus
Diabetes Mellitus - pathology
DNA
Elasticity
Extracellular matrix
Extracellular Matrix - drug effects
Extracellular Matrix - metabolism
Fibroblasts
Fibroblasts - drug effects
Fibroblasts - metabolism
Glycosaminoglycans - metabolism
growth factors
Human Umbilical Vein Endothelial Cells - cytology
Human Umbilical Vein Endothelial Cells - drug effects
Humans
hydrogel
Hydrogels
Hydrogels - chemistry
Mesenchymal stem cells
Neovascularization, Physiologic - drug effects
Paracrine Communication - drug effects
Paracrine signalling
Reconstructive surgery
Regeneration
Regenerative medicine
Stability
Stromal cells
Stromal Cells - drug effects
Stromal Cells - metabolism
Sulfates - metabolism
Tissue engineering
Viscoelasticity
Viscosity
Wound healing
wound healing
decellularization
acellular matrix
growth factors
hydrogel
mesenchymal stem cells
ISSN:1932-6254, 1932-7005, 1932-7005
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Shrnutí:Fat grafting is an established clinical intervention to promote tissue repair. The role of the fat's extracellular matrix (ECM) in regeneration is largely neglected. We investigated in vitro the use of human adipose tissue‐derived ECM hydrogels as release platform for factors secreted by adipose‐derived stromal cells (ASCs). Lipoaspirates from nondiabetic and diabetic donors were decellularized. Finely powdered acellular ECM was evaluated for cell remainders and DNA content. Acellular ECM was digested, and hydrogels were formed at 37°C and their viscoelastic relaxation properties investigated. Release of ASC‐released factors from hydrogels was immune assessed, and bio‐activity was determined by fibroblast proliferation and migration and endothelial angiogenesis. Acellular ECM contained no detectable cell remainders and negligible DNA contents. Viscoelastic relaxation measurements yielded no data for diabetic‐derived hydrogels due to gel instability. Hydrogels released several ASC‐released factors concurrently in a sustained fashion. Functionally, released factors stimulated fibroblast proliferation and migration as well as angiogenesis. No difference between nondiabetic and diabetic hydrogels in release of factors was measured. Adipose ECM hydrogels incubated with released factors by ASC are a promising new therapeutic modality to promote several important wound healing‐related processes by releasing factors in a controlled way.
Bibliografie:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:1932-6254
1932-7005
1932-7005
DOI:10.1002/term.2843