An imaging mass cytometry immunophenotyping panel for non-human primate tissues
It has recently become clear that spatial organization contributes to cellular function and that expanding our knowledge on cellular organization is essential to further our understanding of processes in health and disease. Imaging mass cytometry enables high dimensional imaging of tissue while pres...
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| Veröffentlicht in: | Frontiers in immunology Jg. 13; S. 915157 |
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| Format: | Journal Article |
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Frontiers Media S.A
15.07.2022
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| Abstract | It has recently become clear that spatial organization contributes to cellular function and that expanding our knowledge on cellular organization is essential to further our understanding of processes in health and disease. Imaging mass cytometry enables high dimensional imaging of tissue while preserving spatial context and is therefore a suitable tool to unravel spatial relationships between cells. As availability of human tissue collected over the course of disease or infection is limited, preclinical models are a valuable source of such material. Non-human primate models are used for translational research as their anatomy, physiology and immune system closely resemble those of humans due to close evolutionary proximity. Tissue from non-human primate studies is often preserved large archives encompassing a range of conditions and organs. However, knowledge on antibody clones suitable for FFPE tissue of non-human primate origin is very limited. Here, we present an imaging mass cytometry panel development pipeline which enables the selection and incorporation of antibodies for imaging of non-human primate tissue. This has resulted in an 18-marker backbone panel which enables visualization of a broad range of leukocyte subsets in rhesus and cynomolgus macaque tissues. This high-dimensional imaging mass cytometry panel can be used to increase our knowledge of cellular organization within tissues and its effect on outcome of disease. |
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| AbstractList | It has recently become clear that spatial organization contributes to cellular function and that expanding our knowledge on cellular organization is essential to further our understanding of processes in health and disease. Imaging mass cytometry enables high dimensional imaging of tissue while preserving spatial context and is therefore a suitable tool to unravel spatial relationships between cells. As availability of human tissue collected over the course of disease or infection is limited, preclinical models are a valuable source of such material. Non-human primate models are used for translational research as their anatomy, physiology and immune system closely resemble those of humans due to close evolutionary proximity. Tissue from non-human primate studies is often preserved large archives encompassing a range of conditions and organs. However, knowledge on antibody clones suitable for FFPE tissue of non-human primate origin is very limited. Here, we present an imaging mass cytometry panel development pipeline which enables the selection and incorporation of antibodies for imaging of non-human primate tissue. This has resulted in an 18-marker backbone panel which enables visualization of a broad range of leukocyte subsets in rhesus and cynomolgus macaque tissues. This high-dimensional imaging mass cytometry panel can be used to increase our knowledge of cellular organization within tissues and its effect on outcome of disease. It has recently become clear that spatial organization contributes to cellular function and that expanding our knowledge on cellular organization is essential to further our understanding of processes in health and disease. Imaging mass cytometry enables high dimensional imaging of tissue while preserving spatial context and is therefore a suitable tool to unravel spatial relationships between cells. As availability of human tissue collected over the course of disease or infection is limited, preclinical models are a valuable source of such material. Non-human primate models are used for translational research as their anatomy, physiology and immune system closely resemble those of humans due to close evolutionary proximity. Tissue from non-human primate studies is often preserved large archives encompassing a range of conditions and organs. However, knowledge on antibody clones suitable for FFPE tissue of non-human primate origin is very limited. Here, we present an imaging mass cytometry panel development pipeline which enables the selection and incorporation of antibodies for imaging of non-human primate tissue. This has resulted in an 18-marker backbone panel which enables visualization of a broad range of leukocyte subsets in rhesus and cynomolgus macaque tissues. This high-dimensional imaging mass cytometry panel can be used to increase our knowledge of cellular organization within tissues and its effect on outcome of disease.It has recently become clear that spatial organization contributes to cellular function and that expanding our knowledge on cellular organization is essential to further our understanding of processes in health and disease. Imaging mass cytometry enables high dimensional imaging of tissue while preserving spatial context and is therefore a suitable tool to unravel spatial relationships between cells. As availability of human tissue collected over the course of disease or infection is limited, preclinical models are a valuable source of such material. Non-human primate models are used for translational research as their anatomy, physiology and immune system closely resemble those of humans due to close evolutionary proximity. Tissue from non-human primate studies is often preserved large archives encompassing a range of conditions and organs. However, knowledge on antibody clones suitable for FFPE tissue of non-human primate origin is very limited. Here, we present an imaging mass cytometry panel development pipeline which enables the selection and incorporation of antibodies for imaging of non-human primate tissue. This has resulted in an 18-marker backbone panel which enables visualization of a broad range of leukocyte subsets in rhesus and cynomolgus macaque tissues. This high-dimensional imaging mass cytometry panel can be used to increase our knowledge of cellular organization within tissues and its effect on outcome of disease. |
| Author | Joosten, Simone A. Ijsselsteijn, Marieke E. Verreck, Frank A. W. Niewold, Paula Ottenhoff, Tom H. M. |
| AuthorAffiliation | 2 Department of Pathology, Leiden University Medical Centre , Leiden , Netherlands 1 Department of Infectious Diseases, Leiden University Medical Centre , Leiden , Netherlands 3 Section of Tuberculosis (TB) Research and Immunology, Department of Parasitology, Biomedical Primate Research Centre , Rijswijk , Netherlands |
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| Cites_doi | 10.1038/s41590-021-01121-x 10.1089/bio.2012.0052 10.1128/microbiolspec.TBTB2-0007-2016 10.1371/journal.pbio.2000719 10.1038/nmeth.2869 10.3389/fmicb.2021.626553 10.1093/ilar/ilx021 10.3389/fimmu.2019.02534 10.1038/s41586-019-1876-x 10.1038/s41577-018-0005-7 |
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| Copyright | Copyright © 2022 Niewold, Ijsselsteijn, Verreck, Ottenhoff and Joosten. Copyright © 2022 Niewold, Ijsselsteijn, Verreck, Ottenhoff and Joosten 2022 Niewold, Ijsselsteijn, Verreck, Ottenhoff and Joosten |
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| References | Shou (B4) 2021; 12 Estes (B5) 2018; 18 McCaffrey (B3) 2022 Peña (B7) 2016; 4 Emborg (B6) 2017; 58 Ijsselsteijn (B10) 2019; 10 Kokkat (B8) 2013; 11 Giesen (B1) 2014; 11 Jackson (B2) 2020; 578 Morrissey (B9) 2017; 15 |
| References_xml | – year: 2022 ident: B3 article-title: The immunoregulatory landscape of human tuberculosis granulomas publication-title: Nat Immunol doi: 10.1038/s41590-021-01121-x – volume: 11 year: 2013 ident: B8 article-title: Archived formalin-fixed paraffin-embedded (FFPE) blocks: A valuable underexploited resource for extraction of DNA, RNA, and protein publication-title: Biopreserv Biobanking doi: 10.1089/bio.2012.0052 – volume: 4 year: 2016 ident: B7 article-title: Non-human primate models of tuberculosis publication-title: Microbiol Spectr doi: 10.1128/microbiolspec.TBTB2-0007-2016 – volume: 15 year: 2017 ident: B9 article-title: The sharing experimental animal resources, coordinating holdings (SEARCH) framework: Encouraging reduction, replacement, and refinement in animal research publication-title: PLoS Biol doi: 10.1371/journal.pbio.2000719 – volume: 11 year: 2014 ident: B1 article-title: Highly multiplexed imaging of tumor tissues with subcellular resolution by mass cytometry publication-title: Nat Methods doi: 10.1038/nmeth.2869 – volume: 12 year: 2021 ident: B4 article-title: Animal models for COVID-19: Hamsters, mouse, ferret, mink, tree shrew, and non-human primates publication-title: Front Microbiol doi: 10.3389/fmicb.2021.626553 – volume: 58 start-page: 190 year: 2017 ident: B6 article-title: Nonhuman primate models of neurodegenerative disorders publication-title: ILAR J doi: 10.1093/ilar/ilx021 – volume: 10 year: 2019 ident: B10 article-title: A 40-marker panel for high dimensional characterization of cancer immune microenvironments by imaging mass cytometry publication-title: Front Immunol doi: 10.3389/fimmu.2019.02534 – volume: 578 year: 2020 ident: B2 article-title: The single-cell pathology landscape of breast cancer publication-title: Nature doi: 10.1038/s41586-019-1876-x – volume: 18 start-page: 390 year: 2018 ident: B5 article-title: Nonhuman primate models of human viral infections publication-title: Nat Rev Immunol doi: 10.1038/s41577-018-0005-7 |
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