Infectious salmon anaemia virus (ISAV) in experimentally challenged Atlantic cod (Gadus morhua)

Juvenile Atlantic cod, Gadus morhua, (6 g) were challenged with infectious salmon anaemia virus (ISAV) either by intraperitoneal (i.p.) injection or by cohabitation with ISA-diseased Atlantic salmon (Salmo salar). Samplings of cod were performed over a period of 45 days and various tissue samples we...

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Bibliographic Details
Published in:Archives of virology Vol. 152; no. 10; pp. 1829 - 1837
Main Authors: Grove, S, Hjortaas, M. J, Reitan, L. J, Dannevig, B. H
Format: Journal Article
Language:English
Published: Wien Vienna : Springer-Verlag 01.10.2007
New York, NY Springer
Springer Nature B.V
Subjects:
abnormal behavior
analysis
Animals
Base Sequence
Biological and medical sciences
brain
Brain - virology
Cell Line
fish
Fish Diseases
Fish Diseases - virology
Fundamental and applied biological sciences. Psychology
Gadus morhua
Gadus morhua - virology
Genes, Viral
genetics
Heart
Heart - virology
Infectious salmon anemia virus
Intestines
Intestines - virology
Isavirus
Isavirus - genetics
Isavirus - isolation & purification
Isavirus - pathogenicity
Isavirus - physiology
isolation & purification
Kidney
Kidney - virology
Microbiology
Miscellaneous
Molecular Sequence Data
mortality
Orthomyxoviridae Infections
Orthomyxoviridae Infections - veterinary
Orthomyxoviridae Infections - virology
pathogenicity
physiology
Reverse Transcriptase Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction - veterinary
RNA
RNA, Viral
RNA, Viral - analysis
Salmo salar
Salmon isavirus
Spleen
Spleen - virology
Time Factors
Tissue Distribution
veterinary
Virology
Virus Replication
Infection
Virus
Vertebrata
Anemia
Pisces
Hemopathy
Gadus morhua
ISSN:0304-8608, 1432-8798
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Summary:Juvenile Atlantic cod, Gadus morhua, (6 g) were challenged with infectious salmon anaemia virus (ISAV) either by intraperitoneal (i.p.) injection or by cohabitation with ISA-diseased Atlantic salmon (Salmo salar). Samplings of cod were performed over a period of 45 days and various tissue samples were collected. The presence of ISAV RNA (segment 8) in samples was assessed by both conventional RT-PCR and a competitive quantitative real-time RT-PCR. In the i.p.-challenged group, ISAV RNA was detected in fish from all samplings, i.e. at days 7, 15, 21, 30 and 45 post-challenge. At day 7 post-challenge, all individual fish were positive, and so were the vast majority of individual tissue samples. At later samplings, the fraction of positive brain samples remained high (~75%). In contrast, the positive fraction of other tissues/organs declined during the experiment. Analysis of positive brain samples by a quantitative real-time RT-PCR analysis showed that the level of ISAV RNA increased significantly (~20 times) between days 7 and 30 post-challenge and remained high at day 45, indicating that a replication of ISAV had taken place. ISAV RNA was not detected in any control or cohabitation-challenged fish. No abnormal behaviour, clinical disease or, most notably, mortality was observed in any of the challenge or control groups.
Bibliography:http://dx.doi.org/10.1007/s00705-007-1016-z
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ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-007-1016-z