Infectious salmon anaemia virus (ISAV) in experimentally challenged Atlantic cod (Gadus morhua)

Juvenile Atlantic cod, Gadus morhua, (6 g) were challenged with infectious salmon anaemia virus (ISAV) either by intraperitoneal (i.p.) injection or by cohabitation with ISA-diseased Atlantic salmon (Salmo salar). Samplings of cod were performed over a period of 45 days and various tissue samples we...

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Vydáno v:	Archives of virology Ročník 152; číslo 10; s. 1829 - 1837
Hlavní autoři:	Grove, S, Hjortaas, M. J, Reitan, L. J, Dannevig, B. H
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	Wien Vienna : Springer-Verlag 01.10.2007 New York, NY Springer Springer Nature B.V
Témata:	abnormal behavior analysis Animals Base Sequence Biological and medical sciences brain Brain - virology Cell Line fish Fish Diseases Fish Diseases - virology Fundamental and applied biological sciences. Psychology Gadus morhua Gadus morhua - virology Genes, Viral genetics Heart Heart - virology Infectious salmon anemia virus Intestines Intestines - virology Isavirus Isavirus - genetics Isavirus - isolation & purification Isavirus - pathogenicity Isavirus - physiology isolation & purification Kidney Kidney - virology Microbiology Miscellaneous Molecular Sequence Data mortality Orthomyxoviridae Infections Orthomyxoviridae Infections - veterinary Orthomyxoviridae Infections - virology pathogenicity physiology Reverse Transcriptase Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction - veterinary RNA RNA, Viral RNA, Viral - analysis Salmo salar Salmon isavirus Spleen Spleen - virology Time Factors Tissue Distribution veterinary Virology Virus Replication Infection Virus Vertebrata Anemia Pisces Hemopathy Gadus morhua
ISSN:	0304-8608, 1432-8798
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Shrnutí:	Juvenile Atlantic cod, Gadus morhua, (6 g) were challenged with infectious salmon anaemia virus (ISAV) either by intraperitoneal (i.p.) injection or by cohabitation with ISA-diseased Atlantic salmon (Salmo salar). Samplings of cod were performed over a period of 45 days and various tissue samples were collected. The presence of ISAV RNA (segment 8) in samples was assessed by both conventional RT-PCR and a competitive quantitative real-time RT-PCR. In the i.p.-challenged group, ISAV RNA was detected in fish from all samplings, i.e. at days 7, 15, 21, 30 and 45 post-challenge. At day 7 post-challenge, all individual fish were positive, and so were the vast majority of individual tissue samples. At later samplings, the fraction of positive brain samples remained high (~75%). In contrast, the positive fraction of other tissues/organs declined during the experiment. Analysis of positive brain samples by a quantitative real-time RT-PCR analysis showed that the level of ISAV RNA increased significantly (~20 times) between days 7 and 30 post-challenge and remained high at day 45, indicating that a replication of ISAV had taken place. ISAV RNA was not detected in any control or cohabitation-challenged fish. No abnormal behaviour, clinical disease or, most notably, mortality was observed in any of the challenge or control groups.
Bibliografie:	http://dx.doi.org/10.1007/s00705-007-1016-z ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 14 ObjectType-Article-1 ObjectType-Feature-2 content type line 23
ISSN:	0304-8608 1432-8798
DOI:	10.1007/s00705-007-1016-z