Upregulation of long non-coding RNA MEG3 in type 2 diabetes mellitus complicated with vascular disease: a case–control study

Previous studies have indicated that long non-coding RNAs (lncRNAs) were closely related to diabetes. In this study, we aimed to explore the possible role and mechanism of lncRNA MEG3 in the occurrence and development of type 2 diabetes mellitus (T2DM) and its vascular complications. A case–control...

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Vydané v:Molecular and cellular biochemistry Ročník 473; číslo 1-2; s. 93 - 99
Hlavní autori: Chang, Wei-wei, Zhang, Liu, Yao, Xin-ming, Chen, Yan, Zhu, Li-jun, Fang, Zheng-mei, Zhao, Ying, Yao, Ying-shui, Jin, Yue-long
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: New York Springer US 01.10.2020
Springer
Springer Nature B.V
Predmet:
Aged
Analysis
Biochemistry
Biomedical and Life Sciences
Blood circulation disorders
Blood Glucose - metabolism
Cardiology
Case studies
Case-Control Studies
Development and progression
Diabetes
Diabetes mellitus
Diabetes mellitus (non-insulin dependent)
Diabetes Mellitus, Type 2 - blood
Diabetes therapy
Diabetic Angiopathies - blood
Disease control
Ethylenediaminetetraacetic acid
Female
Glycosylated hemoglobin
Hemoglobin
Humans
Leukocytes (mononuclear)
Life Sciences
Male
Medical Biochemistry
Medical research
Medicine, Experimental
MicroRNAs - blood
Middle Aged
Non-coding RNA
Oncology
Pathogenesis
Peripheral blood mononuclear cells
Polymerase chain reaction
Reverse Transcriptase Polymerase Chain Reaction
RNA
RNA, Long Noncoding - biosynthesis
Type 2 diabetes
Up-Regulation
Vascular diseases
MEG3
Vascular complications
Diabetes mellitus
Long non-coding RNA
ISSN:0300-8177, 1573-4919, 1573-4919
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Shrnutí:Previous studies have indicated that long non-coding RNAs (lncRNAs) were closely related to diabetes. In this study, we aimed to explore the possible role and mechanism of lncRNA MEG3 in the occurrence and development of type 2 diabetes mellitus (T2DM) and its vascular complications. A case–control study involving 115 subjects was conducted, including 53 T2DM patients (37 patients with vascular complication and 16 patients without vascular complications) and 62 healthy subjects. We performed real-time polymerase chain reaction (RT-PCR) analysis of the lncRNA MEG3 and miR-146a levels in peripheral blood mononuclear cells (PBMCs) in the 115 samples. We found that the expression of lncRNA MEG3 was upregulated in the T2DM patients with vascular complication (DC group) compared with T2DM patients without vascular complication (D group) ( P  < 0.05) and the control group ( P  < 0.01). miR-146a levels in DC group were significantly lower compared with control group. There was a significant positive correlation between the expression of lncRNA MEG3 and glucose (GLU) ( r  = 0.301, P  = 0.0011) and hemoglobin A1C (HbA1c) ( r  = 0.477, P  = 0.0006). Our study suggests MEG3 may play as an important role in progression of diabetes-related vascular complications, contributing to a novel understanding of pathogenesis and prognosis for diabetes and its complications.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0300-8177
1573-4919
1573-4919
DOI:10.1007/s11010-020-03810-x