Anisotropic dense collagen hydrogels with two ranges of porosity to mimic the skeletal muscle extracellular matrix
Despite the crucial role of the extracellular matrix (ECM) in the organotypic organization and function of skeletal muscles, most 3D models do not mimic its specific characteristics, namely its biochemical composition, stiffness, anisotropy, and porosity. Here, a novel 3D in vitro model of muscle EC...
Uloženo v:
| Vydáno v: | Biomaterials advances Ročník 144; s. 213219 |
|---|---|
| Hlavní autoři: | , , , , , , |
| Médium: | Journal Article |
| Jazyk: | angličtina |
| Vydáno: |
Netherlands
Elsevier
01.01.2023
|
| Témata: | |
| ISSN: | 2772-9508, 2772-9516, 2772-9508 |
| On-line přístup: | Získat plný text |
| Tagy: |
Přidat tag
Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!
|
| Abstract | Despite the crucial role of the extracellular matrix (ECM) in the organotypic organization and function of skeletal muscles, most 3D models do not mimic its specific characteristics, namely its biochemical composition, stiffness, anisotropy, and porosity. Here, a novel 3D in vitro model of muscle ECM was developed reproducing these four crucial characteristics of the native ECM. An anisotropic hydrogel mimicking the muscle fascia was obtained thanks to unidirectional 3D printing of dense collagen with aligned collagen fibrils. The space between the different layers was tuned to generate an intrinsic network of pores (100 μm) suitable for nutrient and oxygen diffusion. By modulating the gelling conditions, the mechanical properties of the construct reached those measured in the physiological muscle ECM. This artificial matrix was thus evaluated for myoblast differentiation. The addition of large channels (600 μm) by molding permitted to create a second range of porosity suitable for cell colonization without altering the physical properties of the hydrogel. Skeletal myoblasts embedded in Matrigel®, seeded within the channels, organized in 3D, and differentiated into multinucleated myotubes. These results show that porous and anisotropic dense collagen hydrogels are promising biomaterials to model skeletal muscle ECM. |
|---|---|
| AbstractList | Despite the crucial role of the extracellular matrix (ECM) in the organotypic organization and function of skeletal muscles, most 3D models do not mimic its specific characteristics, namely its biochemical composition, stiffness, anisotropy, and porosity. Here, a novel 3D in vitro model of muscle ECM was developed reproducing these four crucial characteristics of the native ECM. An anisotropic hydrogel mimicking the muscle fascia was obtained thanks to unidirectional 3D printing of dense collagen with aligned collagen fibrils. The space between the different layers was tuned to generate an intrinsic network of pores (100 μm) suitable for nutrient and oxygen diffusion. By modulating the gelling conditions, the mechanical properties of the construct reached those measured in the physiological muscle ECM. This artificial matrix was thus evaluated for myoblast differentiation. The addition of large channels (600 μm) by molding permitted to create a second range of porosity suitable for cell colonization without altering the physical properties of the hydrogel. Skeletal myoblasts embedded in Matrigel®, seeded within the channels, organized in 3D, and differentiated into multinucleated myotubes. These results show that porous and anisotropic dense collagen hydrogels are promising biomaterials to model skeletal muscle ECM. Despite the crucial role of the extracellular matrix (ECM) in the organotypic organization and function of skeletal muscles, most 3D models do not mimic its specific characteristics, namely its biochemical composition, stiffness, anisotropy, and porosity. Here, a novel 3D in vitro model of muscle ECM was developed reproducing these four crucial characteristics of the native ECM. An anisotropic hydrogel mimicking the muscle fascia was obtained thanks to unidirectional 3D printing of dense collagen with aligned collagen fibrils. The space between the different layers was tuned to generate an intrinsic network of pores (100 μm) suitable for nutrient and oxygen diffusion. By modulating the gelling conditions, the mechanical properties of the construct reached those measured in the physiological muscle ECM. This artificial matrix was thus evaluated for myoblast differentiation. The addition of large channels (600 μm) by molding permitted to create a second range of porosity suitable for cell colonization without altering the physical properties of the hydrogel. Skeletal myoblasts embedded in Matrigel®, seeded within the channels, organized in 3D, and differentiated into multinucleated myotubes. These results show that porous and anisotropic dense collagen hydrogels are promising biomaterials to model skeletal muscle ECM.Despite the crucial role of the extracellular matrix (ECM) in the organotypic organization and function of skeletal muscles, most 3D models do not mimic its specific characteristics, namely its biochemical composition, stiffness, anisotropy, and porosity. Here, a novel 3D in vitro model of muscle ECM was developed reproducing these four crucial characteristics of the native ECM. An anisotropic hydrogel mimicking the muscle fascia was obtained thanks to unidirectional 3D printing of dense collagen with aligned collagen fibrils. The space between the different layers was tuned to generate an intrinsic network of pores (100 μm) suitable for nutrient and oxygen diffusion. By modulating the gelling conditions, the mechanical properties of the construct reached those measured in the physiological muscle ECM. This artificial matrix was thus evaluated for myoblast differentiation. The addition of large channels (600 μm) by molding permitted to create a second range of porosity suitable for cell colonization without altering the physical properties of the hydrogel. Skeletal myoblasts embedded in Matrigel®, seeded within the channels, organized in 3D, and differentiated into multinucleated myotubes. These results show that porous and anisotropic dense collagen hydrogels are promising biomaterials to model skeletal muscle ECM. Despite the crucial role of the extracellular matrix (ECM) in the organotypic organization and function of skeletal muscles, most 3D models do not mimic its specific characteristics, namely its biochemical composition, stiffness, anisotropy, and porosity. Here, a novel 3D in vitro model of muscle ECM was developed reproducing these four crucial characteristics of the native ECM. An anisotropic hydrogel mimicking the muscle fascia was obtained thanks to unidirectional 3D printing of dense collagen with aligned collagen fibrils. The space between the different layers was tuned to generate an intrinsic network of pores (100 μm) suitable for nutrient and oxygen diffusion. By modulating the gelling conditions, the mechanical properties of the construct reached those measured in the physiological muscle ECM. This artificial matrix was thus evaluated for myoblast differentiation. The addition of large channels (600 μm) by molding permitted to create a second range of porosity suitable for cell colonization without altering the physical properties of the hydrogel. Skeletal myoblasts embedded in Matrigel®, seeded within the channels, organized in 3D, and differentiated into multinucleated myotubes. These results show that porous and anisotropic dense collagen hydrogels are promising biomaterials to model skeletal muscle ECM. |
| Author | Dumont, Julien Hélary, Christophe Joanne, Pierre Agbulut, Onnik Brun, Julie Marcellan, Alba Camman, Marie |
| Author_xml | – sequence: 1 givenname: Marie surname: Camman fullname: Camman, Marie organization: Laboratoire de Chimie de la Matière Condensée de Paris, Sorbonne Université, CNRS, UMR 7574, F-75005, Paris, France; Institut de Biologie Paris-Seine (IBPS), Sorbonne Université, CNRS, UMR 8256, Inserm U1164, Biological Adaptation and Ageing, F-75005, Paris, France – sequence: 2 givenname: Pierre surname: Joanne fullname: Joanne, Pierre organization: Institut de Biologie Paris-Seine (IBPS), Sorbonne Université, CNRS, UMR 8256, Inserm U1164, Biological Adaptation and Ageing, F-75005, Paris, France – sequence: 3 givenname: Julie surname: Brun fullname: Brun, Julie organization: Sciences et Ingénierie de la Matière Molle, ESPCI Paris, Université PSL, CNRS, Sorbonne Université, F-75005, Paris, France – sequence: 4 givenname: Alba surname: Marcellan fullname: Marcellan, Alba organization: Sciences et Ingénierie de la Matière Molle, ESPCI Paris, Université PSL, CNRS, Sorbonne Université, F-75005, Paris, France – sequence: 5 givenname: Julien surname: Dumont fullname: Dumont, Julien organization: CIRB Microscopy facility, Collège de France, CNRS, UMR 7241, Inserm U1050, F-75005, Paris, France – sequence: 6 givenname: Onnik surname: Agbulut fullname: Agbulut, Onnik email: onnik.agbulut@sorbonne-universite.fr organization: Institut de Biologie Paris-Seine (IBPS), Sorbonne Université, CNRS, UMR 8256, Inserm U1164, Biological Adaptation and Ageing, F-75005, Paris, France. Electronic address: onnik.agbulut@sorbonne-universite.fr – sequence: 7 givenname: Christophe surname: Hélary fullname: Hélary, Christophe email: christophe.helary@sorbonne-universite.fr organization: Laboratoire de Chimie de la Matière Condensée de Paris, Sorbonne Université, CNRS, UMR 7574, F-75005, Paris, France. Electronic address: christophe.helary@sorbonne-universite.fr |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/36481519$$D View this record in MEDLINE/PubMed https://hal.sorbonne-universite.fr/hal-03894624$$DView record in HAL |
| BookMark | eNpNkEtPWzEQRq2KqlDKP6gqL9tFgt-PZYRoQYrUDV1f-TFJTH2vU9sXyL9vELRiNaPR-Y5G30d0MpUJEPpMyZISqi7vlz4VFx-WjDC2ZJQzat-hM6Y1W1hJzMmb_RRdtHZPCOGMKyn5B3TKlTBUUnuG6mpKrfRa9ingCFMDHErObgsT3h1iLVvIDT-mvsP9seDqpi00XDZ4X2ppqR9wL3hM4zHdd4Dbb8jQXcbj3EIGDE-9ugA5z9lVPLpe09Mn9H7jcoOL13mOfn2_vru6Wax__ri9Wq0XQQjWF9ZTJq3hzjAVtCEkRAVKB6uMoE4QH7X32gbjhdJOyY3xgUsJPkoudYz8HH178e5cHvY1ja4ehuLScLNaD883wo0ViokHemS_vrD7Wv7M0Powpvb8t5ugzG1gWnJOKRX2iH55RWc_Qvxv_tcp_wuIp356 |
| CitedBy_id | crossref_primary_10_1039_D3BM01025B crossref_primary_10_1016_j_eurpolymj_2024_112906 crossref_primary_10_1016_j_ijbiomac_2024_135546 crossref_primary_10_1002_adma_202502279 crossref_primary_10_1002_mame_202300029 crossref_primary_10_1016_j_polymer_2025_128990 crossref_primary_10_1002_jsfa_70112 crossref_primary_10_3390_polym15204052 crossref_primary_10_1038_s41598_025_03504_8 crossref_primary_10_1093_rb_rbaf059 crossref_primary_10_1016_j_microc_2024_112296 crossref_primary_10_34133_cbsystems_0279 crossref_primary_10_1016_j_celbio_2025_100127 crossref_primary_10_1016_j_jmbbm_2025_106922 |
| ContentType | Journal Article |
| Copyright | Copyright © 2022 Elsevier B.V. All rights reserved. Distributed under a Creative Commons Attribution 4.0 International License |
| Copyright_xml | – notice: Copyright © 2022 Elsevier B.V. All rights reserved. – notice: Distributed under a Creative Commons Attribution 4.0 International License |
| DBID | CGR CUY CVF ECM EIF NPM 7X8 1XC VOOES |
| DOI | 10.1016/j.bioadv.2022.213219 |
| DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic Hyper Article en Ligne (HAL) Hyper Article en Ligne (HAL) (Open Access) |
| DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic |
| DatabaseTitleList | MEDLINE MEDLINE - Academic |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: 7X8 name: MEDLINE - Academic url: https://search.proquest.com/medline sourceTypes: Aggregation Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Anatomy & Physiology |
| EISSN | 2772-9508 |
| ExternalDocumentID | oai:HAL:hal-03894624v1 36481519 |
| Genre | Journal Article |
| GroupedDBID | 53G AABNK AALRI AAXUO ABJNI ACRLP AEIPS AEZYN AFJKZ AFRZQ AIKHN AITUG AKRWK ALMA_UNASSIGNED_HOLDINGS AMRAJ ANKPU CGR CUY CVF EBS ECM EFJIC EIF FDB FYGXN M41 NPM ROL SPC SSM 0R~ 7X8 AATTM AAYWO ACLOT ACVFH ADCNI AEUPX AFPUW AIGII AIIUN AKBMS AKYEP APXCP EFKBS 1XC VOOES |
| ID | FETCH-LOGICAL-c442t-9b125983a826c7800cd6e67c96841a40bd7bb79c8b467a65f8bc355ebd5357dd3 |
| ISICitedReferencesCount | 17 |
| ISICitedReferencesURI | http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=001030398200001&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| ISSN | 2772-9508 2772-9516 |
| IngestDate | Mon Nov 03 06:45:39 EST 2025 Thu Oct 02 07:01:43 EDT 2025 Wed Feb 19 02:26:00 EST 2025 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Keywords | Dense collagen Porosity Anisotropy 3D printing Muscle extracellular matrix |
| Language | English |
| License | Copyright © 2022 Elsevier B.V. All rights reserved. Distributed under a Creative Commons Attribution 4.0 International License: http://creativecommons.org/licenses/by/4.0 |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-c442t-9b125983a826c7800cd6e67c96841a40bd7bb79c8b467a65f8bc355ebd5357dd3 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| ORCID | 0000-0002-0788-2320 0000-0001-9312-7278 0000-0002-5873-7000 0000-0001-5098-4835 |
| OpenAccessLink | https://hal.sorbonne-universite.fr/hal-03894624 |
| PMID | 36481519 |
| PQID | 2753311149 |
| PQPubID | 23479 |
| ParticipantIDs | hal_primary_oai_HAL_hal_03894624v1 proquest_miscellaneous_2753311149 pubmed_primary_36481519 |
| PublicationCentury | 2000 |
| PublicationDate | 2023-Jan 20230101 2023-01 |
| PublicationDateYYYYMMDD | 2023-01-01 |
| PublicationDate_xml | – month: 01 year: 2023 text: 2023-Jan |
| PublicationDecade | 2020 |
| PublicationPlace | Netherlands |
| PublicationPlace_xml | – name: Netherlands |
| PublicationTitle | Biomaterials advances |
| PublicationTitleAlternate | Biomater Adv |
| PublicationYear | 2023 |
| Publisher | Elsevier |
| Publisher_xml | – name: Elsevier |
| SSID | ssj0003236553 ssib050729471 |
| Score | 2.3252633 |
| Snippet | Despite the crucial role of the extracellular matrix (ECM) in the organotypic organization and function of skeletal muscles, most 3D models do not mimic its... |
| SourceID | hal proquest pubmed |
| SourceType | Open Access Repository Aggregation Database Index Database |
| StartPage | 213219 |
| SubjectTerms | Anisotropy Bioengineering Chemical Sciences Collagen - analysis Extracellular Matrix - chemistry Hydrogels - analysis Life Sciences Material chemistry Muscle, Skeletal Porosity |
| Title | Anisotropic dense collagen hydrogels with two ranges of porosity to mimic the skeletal muscle extracellular matrix |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/36481519 https://www.proquest.com/docview/2753311149 https://hal.sorbonne-universite.fr/hal-03894624 |
| Volume | 144 |
| WOSCitedRecordID | wos001030398200001&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| journalDatabaseRights | – providerCode: PRVHPJ databaseName: ROAD: Directory of Open Access Scholarly Resources (ISSN International Center) customDbUrl: eissn: 2772-9508 dateEnd: 99991231 omitProxy: false ssIdentifier: ssib050729471 issn: 2772-9508 databaseCode: M~E dateStart: 20220101 isFulltext: true titleUrlDefault: https://road.issn.org providerName: ISSN International Centre |
| link | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV1Lj9MwELbKwoELApZHeawMQlyqrFrHefgYLbsqYltVYpF6i2wnoYE2rtK0dC_8H_4lYztpU1ZIy4GLVblVUmc-jb8ZfzNB6F0GLl_0fe7wJCAOlQPqMJ8wJ4PYuc-JL0KZmJdNBONxOJ2ySafzq6mF2cyDogi3W7b8r6aGOTC2Lp39B3PvLgoT8BmMDiOYHcZbGT4q8pWqSrXMZQ-cipGjg63h973ZdVKqr7Ab1unXH6pX6uICI-cAIq6MQAPY6CLXknnNSVffYV_SBZOL9Qpu1ANfXnKd7Tfy1YVu8L89OBjOFUzadTYCgx1tP-OLOuE60iH6Xr3DC5tYncAmXbbO-ddFU8PdkuiawwZ7mWgueDtvQdw_8hZNQc3e3xEg-g4QvrozdnuuHx44bNsx8obzt3mIb6ciV7A-iP0JOSUQbdc--aDX9jD6HE8-XMSXH8efDr9tCRSH0SWMMz53dBtC6hO6gUD7Lgk8ph3o6Od547o83YCd1sxakwCXuL5neqDultUUbhp14c3_CORmprW4fwt0DOG5eoge1JEKjizCHqFOWjxGx1HBK7W4xu-x0Q6bQ5ljVLZAhw3ocAM6vAMd1qDDADpsQYdVhhvQ4UphAzoMoMMN6LAFHT4AHbage4K-XJxfnQ2d-m0ejqSUVA4TwKVZ6HIIaGUAcYpM_NQPJPNDOuC0L5JAiIDJUMDezX0vC4UEMpyKxHO9IEncp-ioUEX6HOHMTRjtE0m5cKmfAcWnMiVUV2Fzksmki97Co4yXtl9LrDuogyljPbc3ZBe9aZ50DC7VQjdV61UM9nVd4ACUddEza4LdtVxfdzcasBe3ucVLdH-P_FfoqCrX6Wt0T26qfFWeoDvBNDwxMIJxPBn9BgPtpHU |
| linkProvider | ISSN International Centre |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Anisotropic+dense+collagen+hydrogels+with+two+ranges+of+porosity+to+mimic+the+skeletal+muscle+extracellular+matrix&rft.jtitle=Biomaterials+advances&rft.au=Camman%2C+Marie&rft.au=Joanne%2C+Pierre&rft.au=Brun%2C+Julie&rft.au=Marcellan%2C+Alba&rft.date=2023-01-01&rft.pub=Elsevier&rft.issn=2772-9516&rft.eissn=2772-9508&rft.volume=144&rft_id=info:doi/10.1016%2Fj.bioadv.2022.213219&rft.externalDBID=HAS_PDF_LINK&rft.externalDocID=oai%3AHAL%3Ahal-03894624v1 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2772-9508&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2772-9508&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2772-9508&client=summon |