Dissociable Roles of Pallidal Neuron Subtypes in Regulating Motor Patterns

We have previously established that PV neurons and Npas1 neurons are distinct neuron classes in the external globus pallidus (GPe): they have different topographical, electrophysiological, circuit, and functional properties. Aside from Foxp2 neurons, which are a unique subclass within the Npas1 clas...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:The Journal of neuroscience Jg. 41; H. 18; S. 4036
Hauptverfasser: Cui, Qiaoling, Pamukcu, Arin, Cherian, Suraj, Chang, Isaac Y M, Berceau, Brianna L, Xenias, Harry S, Higgs, Matthew H, Rajamanickam, Shivakumar, Chen, Yi, Du, Xixun, Zhang, Yu, McMorrow, Hayley, Abecassis, Zachary A, Boca, Simina M, Justice, Nicholas J, Wilson, Charles J, Chan, C Savio
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States 05.05.2021
Schlagworte:
Animals
Anxiety - psychology
Basic Helix-Loop-Helix Transcription Factors - genetics
Behavior, Animal
Biomechanical Phenomena
Electrophysiological Phenomena
Female
Globus Pallidus - cytology
Globus Pallidus - physiology
Machine Learning
Male
Mice
Mice, Inbred C57BL
Motor Activity - physiology
Nerve Net - cytology
Nerve Net - physiology
Nerve Tissue Proteins - genetics
Neurons - physiology
Optogenetics
Potassium Channels, Voltage-Gated - genetics
Receptors, Atrial Natriuretic Factor - genetics
body kinematics
behavioral dynamics
reciprocal inhibition
machine learning
local collaterals
arkypallidal neurons
ISSN:1529-2401, 1529-2401
Online-Zugang:Weitere Angaben
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:We have previously established that PV neurons and Npas1 neurons are distinct neuron classes in the external globus pallidus (GPe): they have different topographical, electrophysiological, circuit, and functional properties. Aside from Foxp2 neurons, which are a unique subclass within the Npas1 class, we lack driver lines that effectively capture other GPe neuron subclasses. In this study, we examined the utility of Kcng4-Cre, Npr3-Cre, and Npy2r-Cre mouse lines (both males and females) for the delineation of GPe neuron subtypes. By using these novel driver lines, we have provided the most exhaustive investigation of electrophysiological studies of GPe neuron subtypes to date. Corroborating our prior studies, GPe neurons can be divided into two statistically distinct clusters that map onto PV and Npas1 classes. By combining optogenetics and machine learning-based tracking, we showed that optogenetic perturbation of GPe neuron subtypes generated unique behavioral structures. Our findings further highlighted the dissociable roles of GPe neurons in regulating movement and anxiety-like behavior. We concluded that Npr3 neurons and Kcng4 neurons are distinct subclasses of Npas1 neurons and PV neurons, respectively. Finally, by examining local collateral connectivity, we inferred the circuit mechanisms involved in the motor patterns observed with optogenetic perturbations. In summary, by identifying mouse lines that allow for manipulations of GPe neuron subtypes, we created new opportunities for interrogations of cellular and circuit substrates that can be important for motor function and dysfunction. Within the basal ganglia, the external globus pallidus (GPe) has long been recognized for its involvement in motor control. However, we lacked an understanding of precisely how movement is controlled at the GPe level as a result of its cellular complexity. In this study, by using transgenic and cell-specific approaches, we showed that genetically-defined GPe neuron subtypes have distinct roles in regulating motor patterns. In addition, the contributions of these neuron subtypes are in part shaped by the local, inhibitory connections within the GPe. In sum, we have established the foundation for future investigations of motor function and disease pathophysiology.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1529-2401
1529-2401
DOI:10.1523/JNEUROSCI.2210-20.2021