The Aedes aegypti glutathione transferase family

In this report, we describe the glutathione transferase (GST) gene family in the dengue vector Aedes aegypti and suggest a novel role for a new class of mosquito GSTs. Twenty-six GST genes are present in Ae. aegypti, two of which are alternatively spliced to give a total of 29 transcripts for cytoso...

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Vydáno v:	Insect biochemistry and molecular biology Ročník 37; číslo 10; s. 1026 - 1035
Hlavní autoři:	Lumjuan, Nongkran, Stevenson, Bradley J., Prapanthadara, La-aied, Somboon, Pradya, Brophy, Peter M., Loftus, Brendan J., Severson, David W., Ranson, Hilary
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	England Elsevier Ltd 01.10.2007
Témata:	Aedes Aedes - enzymology Aedes - genetics Aedes aegypti Alternative Splicing Amino Acid Sequence Animals Anopheles gambiae binding properties chemistry DDT DDT (pesticide) Drosophila melanogaster enzymology gene expression genetics Glutathione transferase Glutathione Transferase - chemistry Glutathione Transferase - genetics Glutathione Transferase - metabolism hematin binding Heme Hemin Hemin - metabolism Insect Proteins Insect Proteins - chemistry Insect Proteins - genetics Insect Proteins - metabolism Insecticide resistance metabolism Molecular Sequence Data Multigene Family nucleotide sequences Phylogeny recombinant proteins Sequence Alignment sequence homology Substrate Specificity NADPH DDE DTT EST GST CDNB CHP DCNB Insecticide resistance Anopheles gambiae Glutathione transferase DDT Heme IPTG Aedes aegypti GSH HPLC
ISSN:	0965-1748, 1879-0240
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Shrnutí:	In this report, we describe the glutathione transferase (GST) gene family in the dengue vector Aedes aegypti and suggest a novel role for a new class of mosquito GSTs. Twenty-six GST genes are present in Ae. aegypti, two of which are alternatively spliced to give a total of 29 transcripts for cytosolic GSTs. The six classes identified in other insect species are all represented and, as in Anopheles gambiae, the majority of the mosquito GSTs belong to the insect-specific Delta and Epsilon classes with eight members each. Sixteen secure 1:1 orthologs were identified between GSTs in Ae. aegypti and An. gambiae, but only four of these have recognisable orthologs in Drosophila melanogaster. Three mosquito-specific GSTs were identified which did not belong to any previously recognised GST classes. One of these, GSTx2, has been previously implicated in conferring 1,1,1-trichloro-2,2-bis-( p-chlorophenyl)ethane (DDT) resistance in Ae. aegypti from South America. However, we found no evidence for increased levels of this GST protein in DDT/pyrethroid-resistant populations from Thailand. Furthermore, we show that the recombinant GSTX2-2 protein is unable to metabolise DDT. Interestingly, GSTX2-2 showed an affinity for hematin, and this, together with the restricted distribution of this class to haematophagous insects, may indicate a role for these enzymes in protecting mosquitoes against heme toxicity during blood feeding.
Bibliografie:	http://dx.doi.org/10.1016/j.ibmb.2007.05.018 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0965-1748 1879-0240
DOI:	10.1016/j.ibmb.2007.05.018