Uniform droplet splitting and detection using Lab-on-Chip flow cytometry on a microfluidic PDMS device

A PDMS chip is fabricated using soft lithography and applied to investigate the formation and division of nitrobenzene (NB) droplets in a two-phase system stabilized by oleic acid. Using an integrated on-chip flow cytometer setup, effected with optical fibers, droplet size distributions are analyzed...

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Bibliographic Details
Published in:Sensors and actuators. B, Chemical Vol. 229; pp. 7 - 13
Main Authors: Kunstmann-Olsen, Casper, Hanczyc, Martin M., Hoyland, James, Rasmussen, Steen, Rubahn, Horst-Günter
Format: Journal Article
Language:English
Published: Elsevier B.V 28.06.2016
Subjects:
Devices
Droplets
Flow cytometry
Focusing
Lithography
Microfluidic
Microfluidics
Nitrobenzenes
Optical detection
PDMS device
Silicone resins
Flow cytometry
Optical detection
Droplets
Microfluidic
PDMS device
ISSN:0925-4005, 1873-3077
Online Access:Get full text
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Summary:A PDMS chip is fabricated using soft lithography and applied to investigate the formation and division of nitrobenzene (NB) droplets in a two-phase system stabilized by oleic acid. Using an integrated on-chip flow cytometer setup, effected with optical fibers, droplet size distributions are analyzed in situ based on optical signal intensities. By controlling the hydrodynamic flow focusing, uniform droplets of sizes between 100μm and 300μm are created with precise size control. Cross-flow shearing allows one to divide these droplets into anything from 2 to 9 individual droplets, depending on flow parameters.
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ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2016.01.120