Uniform droplet splitting and detection using Lab-on-Chip flow cytometry on a microfluidic PDMS device

A PDMS chip is fabricated using soft lithography and applied to investigate the formation and division of nitrobenzene (NB) droplets in a two-phase system stabilized by oleic acid. Using an integrated on-chip flow cytometer setup, effected with optical fibers, droplet size distributions are analyzed...

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Vydáno v:Sensors and actuators. B, Chemical Ročník 229; s. 7 - 13
Hlavní autoři: Kunstmann-Olsen, Casper, Hanczyc, Martin M., Hoyland, James, Rasmussen, Steen, Rubahn, Horst-Günter
Médium: Journal Article
Jazyk:angličtina
Vydáno: Elsevier B.V 28.06.2016
Témata:
Devices
Droplets
Flow cytometry
Focusing
Lithography
Microfluidic
Microfluidics
Nitrobenzenes
Optical detection
PDMS device
Silicone resins
Flow cytometry
Optical detection
Droplets
Microfluidic
PDMS device
ISSN:0925-4005, 1873-3077
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Shrnutí:A PDMS chip is fabricated using soft lithography and applied to investigate the formation and division of nitrobenzene (NB) droplets in a two-phase system stabilized by oleic acid. Using an integrated on-chip flow cytometer setup, effected with optical fibers, droplet size distributions are analyzed in situ based on optical signal intensities. By controlling the hydrodynamic flow focusing, uniform droplets of sizes between 100μm and 300μm are created with precise size control. Cross-flow shearing allows one to divide these droplets into anything from 2 to 9 individual droplets, depending on flow parameters.
Bibliografie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2016.01.120