Uniform droplet splitting and detection using Lab-on-Chip flow cytometry on a microfluidic PDMS device

A PDMS chip is fabricated using soft lithography and applied to investigate the formation and division of nitrobenzene (NB) droplets in a two-phase system stabilized by oleic acid. Using an integrated on-chip flow cytometer setup, effected with optical fibers, droplet size distributions are analyzed...

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Veröffentlicht in:Sensors and actuators. B, Chemical Jg. 229; S. 7 - 13
Hauptverfasser: Kunstmann-Olsen, Casper, Hanczyc, Martin M., Hoyland, James, Rasmussen, Steen, Rubahn, Horst-Günter
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Elsevier B.V 28.06.2016
Schlagworte:
Devices
Droplets
Flow cytometry
Focusing
Lithography
Microfluidic
Microfluidics
Nitrobenzenes
Optical detection
PDMS device
Silicone resins
Flow cytometry
Optical detection
Droplets
Microfluidic
PDMS device
ISSN:0925-4005, 1873-3077
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Beschreibung
Zusammenfassung:A PDMS chip is fabricated using soft lithography and applied to investigate the formation and division of nitrobenzene (NB) droplets in a two-phase system stabilized by oleic acid. Using an integrated on-chip flow cytometer setup, effected with optical fibers, droplet size distributions are analyzed in situ based on optical signal intensities. By controlling the hydrodynamic flow focusing, uniform droplets of sizes between 100μm and 300μm are created with precise size control. Cross-flow shearing allows one to divide these droplets into anything from 2 to 9 individual droplets, depending on flow parameters.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2016.01.120