Development and in vitro evaluation of κ-carrageenan based polymeric hybrid nanocomposite scaffolds for bone tissue engineering

The excellent biocompatible and osteogenesis characteristics of porous scaffolds play a vital role in bone regeneration. In this study, we have synthesized polymeric hybrid nanocomposites via free-radical polymerization from carrageenan/acrylic-acid/graphene/hydroxyapatite. Porous hybrid nanocomposi...

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Bibliographic Details
Published in:RSC advances Vol. 10; no. 66; pp. 40529 - 40542
Main Authors: Aslam Khan, Muhammad Umar, Raza, Mohsin Ali, Mehboob, Hassan, Abdul Kadir, Mohammed Rafiq, Abd Razak, Saiful Izwan, Shah, Saqlain A., Iqbal, Muhammad Zahir, Amin, Rashid
Format: Journal Article
Language:English
Published: England Royal Society of Chemistry 06.11.2020
The Royal Society of Chemistry
Subjects:
absorbance
Biocompatibility
Biodegradation
Biomedical materials
bone formation
bones
Carrageenan
Cell adhesion
cell culture
cell viability
Chemical composition
Chemistry
compression strength
Compressive strength
Contact angle
dyes
Fourier transform infrared spectroscopy
Fourier transforms
Free radical polymerization
freeze drying
Functional groups
Graphene
hydrophilicity
Hydroxyapatite
Mechanical properties
mice
Modulus of elasticity
Morphology
Nanocomposites
Optical density
polymerization
polymers
Pore size
Porosity
Regeneration (physiology)
Scaffolds
Scanning electron microscopy
Swelling
Tissue engineering
ISSN:2046-2069, 2046-2069
Online Access:Get full text
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Summary:The excellent biocompatible and osteogenesis characteristics of porous scaffolds play a vital role in bone regeneration. In this study, we have synthesized polymeric hybrid nanocomposites via free-radical polymerization from carrageenan/acrylic-acid/graphene/hydroxyapatite. Porous hybrid nanocomposite scaffolds were fabricated through a freeze-drying method to mimic the structural and chemical composition of natural bone. Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and water contact-angle studies were carried-out for functional groups, surface morphology and hydrophilicity of the materials, followed by biodegradation and swelling analysis. The cell viability, cell culture and proliferation were evaluated against mouse pre-osteoblast ( MC3T3-E1 ) cell lines using neutral red dye assay. The cell adherence and proliferation studies were determined by SEM. Physical characterization including optimum porosity and pore size (49.75% and 0.41 × 10 3 μm 2 ), mechanical properties (compression strength 8.87 MPa and elastic modulus 442.63 MPa), swelling (70.20% at 27 °C and 77.21% at 37 °C) and biodegradation (23.8%) were performed. The results indicated CG- g -AAc-3 with a high optical density and better cell viability. Hence, CG- g -AAc-3 was found to be more efficient for bone regeneration with potential applications in fractured bone regeneration.
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ISSN:2046-2069
2046-2069
DOI:10.1039/D0RA07446B