Human intestinal microbiota composition is associated with local and systemic inflammation in obesity

Objective Intestinal microbiota have been suggested to contribute to the development of obesity, but the mechanism remains elusive. The relationship between microbiota composition, intestinal permeability, and inflammation in nonobese and obese subjects was investigated. Design and Methods Fecal mic...

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Veröffentlicht in:Obesity (Silver Spring, Md.) Jg. 21; H. 12; S. E607 - E615
Hauptverfasser: Verdam, Froukje J., Fuentes, Susana, de Jonge, Charlotte, Zoetendal, Erwin G., Erbil, Runi, Greve, Jan Willem, Buurman, Wim A., de Vos, Willem M., Rensen, Sander S.
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States Blackwell Publishing Ltd 01.12.2013
Schlagworte:
Adult
Age
Body Mass Index
bowel
C-Reactive Protein - metabolism
Comorbidity
Deoxyribonucleic acid
Diabetes
diet-induced obesity
disease
DNA
fecal calprotectin
Feces - chemistry
Feces - microbiology
Female
high-fat diet
human gut microbiota
Humans
Inflammation - microbiology
Insulin resistance
Intestines - microbiology
Leukocyte L1 Antigen Complex - metabolism
Male
Metabolic disorders
mice
Microbiota
Middle Aged
Multivariate analysis
nonalcoholic steatohepatitis
Nutrition research
Obesity
Obesity - microbiology
Oligonucleotide Array Sequence Analysis
Permeability
Phylogenetics
Studies
Urine
weight-loss
Young Adult
Netherlands
ISSN:1930-7381, 1930-739X, 1930-739X
Online-Zugang:Volltext
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Zusammenfassung:Objective Intestinal microbiota have been suggested to contribute to the development of obesity, but the mechanism remains elusive. The relationship between microbiota composition, intestinal permeability, and inflammation in nonobese and obese subjects was investigated. Design and Methods Fecal microbiota composition of 28 subjects (BMI 18.6‐60.3 kg m−2) was analyzed by a phylogenetic profiling microarray. Fecal calprotectin and plasma C‐reactive protein levels were determined to evaluate intestinal and systemic inflammation. Furthermore, HbA1c, and plasma levels of transaminases and lipids were analyzed. Gastroduodenal, small intestinal, and colonic permeability were assessed by a multisaccharide test. Results Based on microbiota composition, the study population segregated into two clusters with predominantly obese (15/19) or exclusively nonobese (9/9) subjects. Whereas intestinal permeability did not differ between clusters, the obese cluster showed reduced bacterial diversity, a decreased Bacteroidetes/Firmicutes ratio, and an increased abundance of potential proinflammatory Proteobacteria. Interestingly, fecal calprotectin was only detectable in subjects within the obese microbiota cluster (n = 8/19, P = 0.02). Plasma C‐reactive protein was also increased in these subjects (P = 0.0005), and correlated with the Bacteroidetes/Firmicutes ratio (rs = −0.41, P = 0.03). Conclusions Intestinal microbiota alterations in obese subjects are associated with local and systemic inflammation, suggesting that the obesity‐related microbiota composition has a proinflammatory effect.
Bibliographie:All authors hereby declare they have no competing financial interests in relation to the work described here.
FJV, CdJ, EGZ, JWG, WAB, WMDV, and SSR conceived the study. Data was collected by FJV, SF, CdJ, EGZ, and RE, analyzed by FJV, SF, EGZ, WAB, and SSR, and interpreted by FJV, SF, CdJ, EGZ, JWG, WAB, WMDV, and SSR. Literature searches were performed by FJV, SF, EGZ, JWG, WAB, WMDV, and SSR. Figures were generated by FJV, SF, and SSR. All authors were involved in writing the article and had final approval of the submitted version.
Disclosure
Funding agencies
This work was financially supported by a Senter Novem IOP genomics grant to WAB and JWG (IGE05012A), a Transnational University Limburg grant and a Dutch Digestive Foundation project grant (WO 09‐46) to SSR, and a Spinoza award to WMDV (NWO).
Author contributions
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ISSN:1930-7381
1930-739X
1930-739X
DOI:10.1002/oby.20466