Detection of CFTR protein in human leukocytes by flow cytometry

Leukocytes have previously been shown to express detectable levels of the protein cystic fibrosis transmembrane conductance regulator (CFTR). This study aims to evaluate the application of flow cytometric (FC) analysis to detect CFTR expression, and changes thereof, in these cells. Aliquots (200 μL)...

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Bibliographic Details
Published in:	Cytometry. Part A Vol. 85; no. 7; pp. 611 - 620
Main Authors:	Johansson, Jan, Vezzalini, Marzia, Verzè, Genny, Caldrer, Sara, Bolognin, Silvia, Buffelli, Mario, Bellisola, Giuseppe, Tridello, Gloria, Assael, Baroukh Maurice, Melotti, Paola, Sorio, Claudio
Format:	Journal Article
Language:	English
Published:	United States 01.07.2014
Subjects:	Adolescent Adult Aged Cell Membrane - metabolism Child cystic fibrosis Cystic Fibrosis - diagnosis Cystic Fibrosis - genetics cystic fibrosis transmembrane conductance regulator Cystic Fibrosis Transmembrane Conductance Regulator - biosynthesis Cystic Fibrosis Transmembrane Conductance Regulator - genetics Female Flow Cytometry - methods Fluorescent Dyes genetic disease Humans leukocytes Leukocytes - metabolism Lymphocytes - metabolism Male Middle Aged Monocytes - metabolism Mutation Neutrophils - metabolism Oxadiazoles - pharmacology Young Adult cystic fibrosis transmembrane conductance regulator leukocytes genetic disease cystic fibrosis
ISSN:	1552-4922, 1552-4930, 1552-4930
Online Access:	Get full text
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