Cytokine secretion profiles of cloned T cells from human aortic atherosclerotic plaques

T cells take part in the chronic inflammatory reaction in atherosclerotic plaques, but their specific role in atherosclerosis has not yet been fully elucidated. Nevertheless, one may anticipate that activated T cells may secrete cytokines capable of modulating the morphology and hence the stability...

Celý popis

Uložené v:
Podrobná bibliografia
Vydané v:The Journal of pathology Ročník 188; číslo 2; s. 174 - 179
Hlavní autori: de Boer, Onno J., van der Wal, Allard C., Verhagen, Claudia E., Becker, Anton E.
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: Chichester, UK John Wiley & Sons, Ltd 01.06.1999
Wiley
Predmet:
Aged
Arteriosclerosis - immunology
atherosclerosis
Atherosclerosis (general aspects, experimental research)
Biological and medical sciences
Blood and lymphatic vessels
Cardiology. Vascular system
CD4-Positive T-Lymphocytes - immunology
CD4-Positive T-Lymphocytes - secretion
CD8-Positive T-Lymphocytes - immunology
CD8-Positive T-Lymphocytes - secretion
Cells, Cultured
Clone Cells
cytokines
Cytokines - analysis
Cytokines - secretion
Cytotoxicity Tests, Immunologic
Flow Cytometry
Humans
Interferon-gamma - secretion
interferon-γ
interleukin-4
Interleukin-4 - secretion
Lymphocyte Activation - immunology
Medical sciences
Middle Aged
T-cell clones
T-Lymphocytes - immunology
T-Lymphocytes - secretion
Th0 cells
Th1 cells
Human
Cell cloning
Cell culture
Pathogenesis
Cytokine
Cardiovascular disease
Cytotoxicity
In vitro
Vascular disease
Interleukin 4
Atherosclerosis
T-Lymphocyte
Inflammatory cell
Gamma interferon
ISSN:0022-3417, 1096-9896
On-line prístup:Získať plný text
Tagy: Pridať tag
Žiadne tagy, Buďte prvý, kto otaguje tento záznam!
Popis
Shrnutí:T cells take part in the chronic inflammatory reaction in atherosclerotic plaques, but their specific role in atherosclerosis has not yet been fully elucidated. Nevertheless, one may anticipate that activated T cells may secrete cytokines capable of modulating the morphology and hence the stability of plaques by regulating cell proliferation, lipid metabolism, and extracellular matrix (ECM) synthesis and/or degradation. This study has been designed to investigate the functional properties of T cells in atherosclerotic lesions. For this purpose, T‐cell clones were generated from atherosclerotic plaques isolated from human aortas obtained at autopsy from six subjects. Cloned cells were activated with PMA and OKT‐3 to initiate cytokine production and cytokine profiles of CD4‐positive clones were measured by ELISA. The majority of the T‐cell clones (125/155, 81 per cent) produced both interferon (IFN)‐γ and interleukin (IL)‐4 (type 0 cytokine profile). Moreover, the production of IFN‐γ was dominant in the majority of these clones. A type 1 cytokine profile (high levels of IFN‐γ and low levels of IL‐4) was found in 17 per cent of the clones (27/155). Only three clones (2 per cent) showed a type 2 cytokine secretion pattern (high levels of IL‐4 and low levels of IFN‐γ). No cytolytic activity could be established in plaque‐derived T cells. Our results show that the T‐cell population in atherosclerotic lesions is heterogeneous, but the most dominant T cell by far is the one with a type 0 cytokine profile. The dominant secretion of IFN‐γ by T‐cell clones suggest an important role for plaque T cells in modulating the growth and differentiation of other cells, such as macrophages and smooth muscle cells in atherosclerotic plaques. Copyright © 1999 John Wiley & Sons, Ltd.
Bibliografia:ark:/67375/WNG-T9THBJL0-0
ArticleID:PATH333
istex:9CB7DD4ADB63499533BD88DCEA3784CCCA93C62C
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0022-3417
1096-9896
DOI:10.1002/(SICI)1096-9896(199906)188:2<174::AID-PATH333>3.0.CO;2-3