Comparison of Bacterial Communities in the Throat Swabs from Healthy Subjects and Pharyngitis Patients by Terminal Restriction Fragment Length Polymorphism

Terminal restriction fragment length polymorphism (T-RFLP) analysis was applied to characterize bacterial flora present in the throats of healthy subjects and pharyngitis patients. The 16S rRNA genes of bacteria present in throat metagenome were amplified by PCR with 6-carboxy-fluorescein (6-FAM)-la...

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Bibliographic Details
Published in:	Applied biochemistry and biotechnology Vol. 167; no. 5; pp. 1459 - 1473
Main Authors:	Balaji, Kannan, Thenmozhi, Ramalingam, Sundaravadivel, Marimuthu, Pandian, Shunmugiah Karutha
Format:	Journal Article
Language:	English
Published:	New York Springer-Verlag 01.07.2012 Springer Nature B.V
Subjects:	Bacteria Bacteria - classification Bacteria - genetics Bacteria - isolation & purification Bacteria - pathogenicity bacterial communities Bacteriology Biochemistry Biodiversity Biotechnology Chemistry Chemistry and Materials Science classification Community structure computer software Enzymes Flora genes genetics Genomes Health Humans isolation & purification Metagenome Metagenome - genetics metagenomics microbiology pathogenicity Pathology patients Pharyngitis Pharyngitis - microbiology Pharynx Pharynx - microbiology Phylogeny Polymerase chain reaction Polymorphism Polymorphism, Restriction Fragment Length restriction fragment length polymorphism ribosomal DNA ribosomal RNA RNA, Bacterial RNA, Bacterial - genetics RNA, Ribosomal, 16S RNA, Ribosomal, 16S - genetics Species diversity Species richness Streptococcus pyogenes throat uncertainty Normal flora Restriction enzymes Pharyngitis Metagenome T-RFLP
ISSN:	0273-2289, 1559-0291, 1559-0291
Online Access:	Get full text
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