Graphene-magnetite as adsorbent for magnetic solid phase extraction of 4-hydroxybenzoic acid and 3,4-dihydroxybenzoic acid in stingless bee honey

•Synthesis of new graphene-magnetite solid phase extraction sorbent (G-Fe3O4-MSPE).•Fast and green extraction method for two phenolic acids in stingless bee honey.•Efficient extraction of phenolic acids with low detection limits (0.08–0.14 µg/g).•A method with good relative recoveries (72.6–110.6%)...

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Vydáno v:	Food chemistry Ročník 265; s. 165 - 172
Hlavní autoři:	Musa, Marina, Wan Ibrahim, Wan Aini, Mohd Marsin, Faridah, Abdul Keyon, Aemi S., Rashidi Nodeh, Hamid
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	England Elsevier Ltd 01.11.2018
Témata:	4-hydroxybenzoic acid adsorbents desorption Graphene-magnetite high performance liquid chromatography Magnetic solid phase extraction magnetism methanol Phenolic acids sodium chloride solid phase extraction solvents sonication Stingless bee honey stingless bees MNPs Graphene-magnetite LVSEM 3,4-DHB 4-HB XRD Magnetic solid phase extraction Stingless bee honey Phenolic acids TGA BET Fe3O4 VSM FTIR FeCl36H2O NaCl HPLC-UV/Vis TEM G-Fe3O4 NH4OH MSPE
ISSN:	0308-8146, 1873-7072, 1873-7072
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Abstract	•Synthesis of new graphene-magnetite solid phase extraction sorbent (G-Fe3O4-MSPE).•Fast and green extraction method for two phenolic acids in stingless bee honey.•Efficient extraction of phenolic acids with low detection limits (0.08–0.14 µg/g).•A method with good relative recoveries (72.6–110.6%) and precision values. Graphene-magnetite composite (G-Fe3O4) was successfully synthesized and applied as adsorbent for magnetic solid phase extraction (MSPE) of two phenolic acids namely 4-hydroxybenzoic acid (4-HB) and 3,4-dihydroxybenzoic acid (3,4-DHB) from stingless bee honey prior to analysis using high performance liquid chromatography with ultraviolet–visible detection (HPLC-UV/Vis). Several MSPE parameters affecting extraction of these two acids were optimized. Optimum MSPE conditions were 50 mg of G-Fe3O4 adsorbent, 5 min extraction time at 1600 rpm, 30 mL sample volume, sample solution pH 0.5, 200 µL methanol as desorption solvent (5 min sonication assisted) and 5% w/v NaCl. The LODs (3 S/N) calculated for 4-HB and 3,4-DHB were 0.08 and 0.14 µg/g, respectively. Good relative recoveries (72.6–110.6%) and reproducibility values (RSD < 8.5%, n = 9) were obtained. The developed G-Fe3O4 MSPE method offered is simple, easy, environmental friendly and efficient for extraction of the two phenolic acids from stingless bee honey samples.
AbstractList	•Synthesis of new graphene-magnetite solid phase extraction sorbent (G-Fe3O4-MSPE).•Fast and green extraction method for two phenolic acids in stingless bee honey.•Efficient extraction of phenolic acids with low detection limits (0.08–0.14 µg/g).•A method with good relative recoveries (72.6–110.6%) and precision values. Graphene-magnetite composite (G-Fe3O4) was successfully synthesized and applied as adsorbent for magnetic solid phase extraction (MSPE) of two phenolic acids namely 4-hydroxybenzoic acid (4-HB) and 3,4-dihydroxybenzoic acid (3,4-DHB) from stingless bee honey prior to analysis using high performance liquid chromatography with ultraviolet–visible detection (HPLC-UV/Vis). Several MSPE parameters affecting extraction of these two acids were optimized. Optimum MSPE conditions were 50 mg of G-Fe3O4 adsorbent, 5 min extraction time at 1600 rpm, 30 mL sample volume, sample solution pH 0.5, 200 µL methanol as desorption solvent (5 min sonication assisted) and 5% w/v NaCl. The LODs (3 S/N) calculated for 4-HB and 3,4-DHB were 0.08 and 0.14 µg/g, respectively. Good relative recoveries (72.6–110.6%) and reproducibility values (RSD < 8.5%, n = 9) were obtained. The developed G-Fe3O4 MSPE method offered is simple, easy, environmental friendly and efficient for extraction of the two phenolic acids from stingless bee honey samples. Graphene-magnetite composite (G-Fe3O4) was successfully synthesized and applied as adsorbent for magnetic solid phase extraction (MSPE) of two phenolic acids namely 4-hydroxybenzoic acid (4-HB) and 3,4-dihydroxybenzoic acid (3,4-DHB) from stingless bee honey prior to analysis using high performance liquid chromatography with ultraviolet–visible detection (HPLC-UV/Vis). Several MSPE parameters affecting extraction of these two acids were optimized. Optimum MSPE conditions were 50 mg of G-Fe3O4 adsorbent, 5 min extraction time at 1600 rpm, 30 mL sample volume, sample solution pH 0.5, 200 µL methanol as desorption solvent (5 min sonication assisted) and 5% w/v NaCl. The LODs (3 S/N) calculated for 4-HB and 3,4-DHB were 0.08 and 0.14 µg/g, respectively. Good relative recoveries (72.6–110.6%) and reproducibility values (RSD < 8.5%, n = 9) were obtained. The developed G-Fe3O4 MSPE method offered is simple, easy, environmental friendly and efficient for extraction of the two phenolic acids from stingless bee honey samples. Graphene-magnetite composite (G-Fe3O4) was successfully synthesized and applied as adsorbent for magnetic solid phase extraction (MSPE) of two phenolic acids namely 4-hydroxybenzoic acid (4-HB) and 3,4-dihydroxybenzoic acid (3,4-DHB) from stingless bee honey prior to analysis using high performance liquid chromatography with ultraviolet-visible detection (HPLC-UV/Vis). Several MSPE parameters affecting extraction of these two acids were optimized. Optimum MSPE conditions were 50 mg of G-Fe3O4 adsorbent, 5 min extraction time at 1600 rpm, 30 mL sample volume, sample solution pH 0.5, 200 µL methanol as desorption solvent (5 min sonication assisted) and 5% w/v NaCl. The LODs (3 S/N) calculated for 4-HB and 3,4-DHB were 0.08 and 0.14 µg/g, respectively. Good relative recoveries (72.6-110.6%) and reproducibility values (RSD < 8.5%, n = 9) were obtained. The developed G-Fe3O4 MSPE method offered is simple, easy, environmental friendly and efficient for extraction of the two phenolic acids from stingless bee honey samples.Graphene-magnetite composite (G-Fe3O4) was successfully synthesized and applied as adsorbent for magnetic solid phase extraction (MSPE) of two phenolic acids namely 4-hydroxybenzoic acid (4-HB) and 3,4-dihydroxybenzoic acid (3,4-DHB) from stingless bee honey prior to analysis using high performance liquid chromatography with ultraviolet-visible detection (HPLC-UV/Vis). Several MSPE parameters affecting extraction of these two acids were optimized. Optimum MSPE conditions were 50 mg of G-Fe3O4 adsorbent, 5 min extraction time at 1600 rpm, 30 mL sample volume, sample solution pH 0.5, 200 µL methanol as desorption solvent (5 min sonication assisted) and 5% w/v NaCl. The LODs (3 S/N) calculated for 4-HB and 3,4-DHB were 0.08 and 0.14 µg/g, respectively. Good relative recoveries (72.6-110.6%) and reproducibility values (RSD < 8.5%, n = 9) were obtained. The developed G-Fe3O4 MSPE method offered is simple, easy, environmental friendly and efficient for extraction of the two phenolic acids from stingless bee honey samples. Graphene-magnetite composite (G-Fe O ) was successfully synthesized and applied as adsorbent for magnetic solid phase extraction (MSPE) of two phenolic acids namely 4-hydroxybenzoic acid (4-HB) and 3,4-dihydroxybenzoic acid (3,4-DHB) from stingless bee honey prior to analysis using high performance liquid chromatography with ultraviolet-visible detection (HPLC-UV/Vis). Several MSPE parameters affecting extraction of these two acids were optimized. Optimum MSPE conditions were 50 mg of G-Fe O adsorbent, 5 min extraction time at 1600 rpm, 30 mL sample volume, sample solution pH 0.5, 200 µL methanol as desorption solvent (5 min sonication assisted) and 5% w/v NaCl. The LODs (3 S/N) calculated for 4-HB and 3,4-DHB were 0.08 and 0.14 µg/g, respectively. Good relative recoveries (72.6-110.6%) and reproducibility values (RSD < 8.5%, n = 9) were obtained. The developed G-Fe O MSPE method offered is simple, easy, environmental friendly and efficient for extraction of the two phenolic acids from stingless bee honey samples.
Author	Rashidi Nodeh, Hamid Musa, Marina Mohd Marsin, Faridah Abdul Keyon, Aemi S. Wan Ibrahim, Wan Aini
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Keywords	MNPs Graphene-magnetite LVSEM 3,4-DHB 4-HB XRD Magnetic solid phase extraction Stingless bee honey Phenolic acids TGA BET Fe3O4 VSM FTIR FeCl36H2O NaCl HPLC-UV/Vis TEM G-Fe3O4 NH4OH MSPE
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Snippet	•Synthesis of new graphene-magnetite solid phase extraction sorbent (G-Fe3O4-MSPE).•Fast and green extraction method for two phenolic acids in stingless bee... Graphene-magnetite composite (G-Fe O ) was successfully synthesized and applied as adsorbent for magnetic solid phase extraction (MSPE) of two phenolic acids... Graphene-magnetite composite (G-Fe3O4) was successfully synthesized and applied as adsorbent for magnetic solid phase extraction (MSPE) of two phenolic acids...
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SubjectTerms	4-hydroxybenzoic acid adsorbents desorption Graphene-magnetite high performance liquid chromatography Magnetic solid phase extraction magnetism methanol Phenolic acids sodium chloride solid phase extraction solvents sonication Stingless bee honey stingless bees
Title	Graphene-magnetite as adsorbent for magnetic solid phase extraction of 4-hydroxybenzoic acid and 3,4-dihydroxybenzoic acid in stingless bee honey
URI	https://dx.doi.org/10.1016/j.foodchem.2018.04.020 https://www.ncbi.nlm.nih.gov/pubmed/29884368 https://www.proquest.com/docview/2052803479 https://www.proquest.com/docview/2116883790
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