Variety of size and form of GRM2 bacterial microcompartment particles

Bacterial microcompartments (BMCs) are bacterial organelles involved in enzymatic processes, such as carbon fixation, choline, ethanolamine and propanediol degradation, and others. Formed of a semi‐permeable protein shell and an enzymatic core, they can enhance enzyme performance and protect the cel...

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Bibliographic Details
Published in:Protein science Vol. 30; no. 5; pp. 1035 - 1043
Main Authors: Cesle, Eva Emilija, Filimonenko, Anatolij, Tars, Kaspars, Kalnins, Gints
Format: Journal Article
Language:English
Published: Hoboken, USA John Wiley & Sons, Inc 01.05.2021
Wiley Subscription Services, Inc
Subjects:
Adaptability
Bacteria
bacterial microcompartments
Bacterial Proteins - chemistry
Bacterial Proteins - metabolism
Biodegradation
Carbon fixation
Choline
Cryoelectron Microscopy
cryo‐EM
Elongated structure
Ethanolamine
Full‐Length Paper
Full‐Length Papers
GRM2
Icosahedral phase
Intermediates
Klebsiella
Klebsiella pneumoniae
Klebsiella pneumoniae - chemistry
Klebsiella pneumoniae - metabolism
Klebsiella pneumoniae - ultrastructure
Organelles
GRM2
bacterial microcompartments
cryo-EM
Klebsiella pneumoniae
ISSN:0961-8368, 1469-896X, 1469-896X
Online Access:Get full text
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Summary:Bacterial microcompartments (BMCs) are bacterial organelles involved in enzymatic processes, such as carbon fixation, choline, ethanolamine and propanediol degradation, and others. Formed of a semi‐permeable protein shell and an enzymatic core, they can enhance enzyme performance and protect the cell from harmful intermediates. With the ability to encapsulate non‐native enzymes, BMCs show high potential for applied use. For this goal, a detailed look into shell form variability is significant to predict shell adaptability. Here we present four novel 3D cryo‐EM maps of recombinant Klebsiella pneumoniae GRM2 BMC shell particles with the resolution in range of 9 to 22 Å and nine novel 2D classes corresponding to discrete BMC shell forms. These structures reveal icosahedral, elongated, oblate, multi‐layered and polyhedral traits of BMCs, indicating considerable variation in size and form as well as adaptability during shell formation processes.
Bibliography:Funding information
Czech Infrastructure for Integrative Structural Biology, MEYS CR, Grant/Award Number: LM2018127; European Regional Development Fund, Grant/Award Number: 1.1.1.2/VIAA/4/20/705; Latvijas Universitātes Fonds
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Funding information Czech Infrastructure for Integrative Structural Biology, MEYS CR, Grant/Award Number: LM2018127; European Regional Development Fund, Grant/Award Number: 1.1.1.2/VIAA/4/20/705; Latvijas Universitātes Fonds
ISSN:0961-8368
1469-896X
1469-896X
DOI:10.1002/pro.4069