The suitability of different cellular in vitro immunotoxicity and genotoxicity methods for the analysis of nanoparticle-induced events

Suitable assays and test strategies are needed to analyze potential genotoxic and immunotoxic health effects caused by nanoparticle exposure. The development and validation of such methods is challenging because nanoparticles may show unexpected behavior, like aggregation or interference with optica...

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Published in:Nanotoxicology Vol. 4; no. 1; pp. 52 - 72
Main Authors: Pfaller, Tobias, Colognato, Renato, Nelissen, Inge, Favilli, Flavia, Casals, Eudald, Ooms, Daniëlla, Leppens, Hilde, Ponti, Jessica, Stritzinger, René, Puntes, Victor, Boraschi, Diana, Duschl, Albert, Oostingh, Gertie J.
Format: Journal Article
Language:English
Published: England Taylor & Francis 01.03.2010
Subjects:
Aggregation behavior
Cell Line
Dendritic Cells - cytology
Dendritic Cells - immunology
DNA Damage
Ferric Compounds - chemistry
Genes, Reporter
genotoxicology
Gold - chemistry
human cells
Humans
Immunity, Innate - immunology
Immunotoxicology
Immunotoxins - chemistry
Immunotoxins - toxicity
in vitro
Interdisciplinary Studies
Intestinal Mucosa - cytology
Intestinal Mucosa - immunology
Lipopolysaccharides - metabolism
Metal Nanoparticles - chemistry
Metal Nanoparticles - toxicity
Monocytes - immunology
Mutagenicity Tests - methods
Oxidative Stress
Reactive Oxygen Species - metabolism
ISSN:1743-5390, 1743-5404, 1743-5404
Online Access:Get full text
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Summary:Suitable assays and test strategies are needed to analyze potential genotoxic and immunotoxic health effects caused by nanoparticle exposure. The development and validation of such methods is challenging because nanoparticles may show unexpected behavior, like aggregation or interference with optical measurements, when routine in vitro assays are performed. In our interdisciplinary study, the effects of inorganic gold (4.5 nm) and iron oxide (7.3 nm) nanoparticles with a narrow size distribution were tested on human cells using different assay systems. The results show that cytotoxicity as well as immunotoxicity and genotoxicity induced by these two inorganic nanoparticles was low or absent when using a panel of cell-based tests in different laboratories. However, several technical issues had to be tackled that were specific for working with nanoparticles. The methods used, their suitability for nanotoxicity testing, and the technical problems encountered are carefully described and discussed in this paper.
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ISSN:1743-5390
1743-5404
1743-5404
DOI:10.3109/17435390903374001