Effect of prestorage leukocyte reduction on proteins of platelets obtained by apheresis

The effect of prestorage leukocyte reduction was evaluated on platelet concentrates (PCs) obtained by apheresis using the 'surge' technique. Two hours after collection, the PCs were divided into 2 equal units. One unit was filtered through a Sepacell PL-10a, producing a filtered PC (FPC)....

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Vydáno v:Vox sanguinis Ročník 65; číslo 4; s. 279
Hlavní autoři: Sarraj-Reguieg, A, Tissot, J D, Hochstrasser, D F, von Fliedner, V, Bachmann, F, Schneider, P
Médium: Journal Article
Jazyk:angličtina
Vydáno: England 01.01.1993
Témata:
Blood Platelets - chemistry
Blood Platelets - drug effects
Blood Preservation
Electrophoresis, Gel, Two-Dimensional
Flow Cytometry
Humans
Leukocyte Count
P-Selectin
Platelet Aggregation - drug effects
Platelet Count
Platelet Membrane Glycoproteins - analysis
Plateletpheresis
Ristocetin - pharmacology
Thrombin - pharmacology
Time Factors
ISSN:0042-9007
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Shrnutí:The effect of prestorage leukocyte reduction was evaluated on platelet concentrates (PCs) obtained by apheresis using the 'surge' technique. Two hours after collection, the PCs were divided into 2 equal units. One unit was filtered through a Sepacell PL-10a, producing a filtered PC (FPC). The second unit constituted a non-filtered PC (NFPC). FPCs and NFPCs were stored at room temperature in 1,400-ml CLX bags on a horizontal agitator up to 7 days. We analyzed platelet samples obtained during storage from NFPCs and FPCs at days 1, 3 and 7. The expression of membrane glycoproteins (GP)Ib and GPIIb/IIIa (assessed by flow cytometry), platelet response to thrombin and ristocetin (aggregometry) and global platelet protein pattern (studied by high-resolution two-dimensional gel electrophoresis) remained stable over the 7 days of storage in NFPCs as well as in FPCs. However, in both preparations, the expression of GMP-140 (flow cytometry) progressively increased during storage. Our in vitro study indicates that early leukocyte reduction by filtration of apheresis PCs does not induce modifications in platelet GPs and protein patterns.
Bibliografie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0042-9007
DOI:10.1111/j.1423-0410.1993.tb02167.x