A one-health sampling strategy to explore the dissemination and relationship between colistin resistance in human, animal, and environmental sectors in laos

This study was designed to investigate the molecular epidemiology ofmobile colistin resistance (mcr)using a “one-health” approach in Laos and to predict whether any dominant plasmid backbone and/or strain type influences the dissemination ofmcr.We collected 673 samples from humans (rectal normal flo...

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Published in:Engineering (Beijing, China) Vol. 15; no. 8; pp. 45 - 56
Main Authors: Zhou, Yuqing, Farzana, Refath, Sihalath, Somsavanh, Rattanavong, Sayaphet, Vongsouvath, Manivanh, Mayxay, Mayfong, Sands, Kirsty, Newton, Paul N., Dance, David A.B., Hassan, Brekhna, Walsh, Timothy R.
Format: Journal Article
Language:English
Published: Elsevier Ltd 01.08.2022
Faculty of Infectious and Tropical Diseases,London School of Hygiene and Tropical Medicine,London WC1E 7HT,UK%Department of Medical Microbiology,Institute of Infection and Immunity,School of Medicine,Cardiff University,Cardiff CF14 4XN,UK%Department of Zoology,University of Oxford,Oxford OX1 3SZ,UK
Centre for Tropical Medicine&Global Health,Nuffield Department of Medicine,University of Oxford,Oxford OX3 7BN,UK
Institute of Research and Education Development,University of Health Sciences,Vientiane PO Box 7444,Lao PDR%Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit,Microbiology Laboratory,Mahosot Hospital,Vientiane PO Box 292,Lao PDR
Department of Zoology,University of Oxford,Oxford OX1 3SZ,UK
Ineos Oxford Institute for Antimicrobial Research(IOI),University of Oxford,Oxford OX1 3RE,UK
Department of Medical Microbiology,Institute of Infection and Immunity,School of Medicine,Cardiff University,Cardiff CF14 4XN,UK%Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit,Microbiology Laboratory,Mahosot Hospital,Vientiane PO Box 292,Lao PDR%Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit,Microbiology Laboratory,Mahosot Hospital,Vientiane PO Box 292,Lao PDR
Elsevier
Subjects:
Colistin resistance
Escherichia coli
Horizontal transmission
Laos
Mcr
Horizontal transmission
Colistin resistance
Mcr
Laos
Escherichia coli
Colistin resistance mcr
ISSN:2095-8099
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Summary:This study was designed to investigate the molecular epidemiology ofmobile colistin resistance (mcr)using a “one-health” approach in Laos and to predict whether any dominant plasmid backbone and/or strain type influences the dissemination ofmcr.We collected 673 samples from humans (rectal normal flora), poultry, and the environment (water, flies, birds, etc.) in Vientiane, Laos, from May to September 2018. A total of 238 Escherichia coli (E. coli)isolated from non-duplicative samples, consisting of 98MCR-positive E. coli(MCRPEC) (“mcr” denotes the gene encoding mobile colistin resistance, and “MCR” denotes the subsequent protein encoded by mcr) and 140MCR-negativeE. coli(MCRNEC), were characterized by phenotype and Illumina sequencing. A subset of MCRPEC was selected for MinION sequencing, conjugation assay, plasmid stability, andgrowth kinetics in vitro. The prevalence of MCRPEC was found to be 14.6% (98/673), with the highest prevalence in human rectal swabs (45.9% (45/98),p < 0.0001, odds ratio (OR): 0.125, 95% CI: 0.077–0.202). The percentages of MCRPEC from other samples were 14.3% (2/14) in dog feces, 12.0% (24/200) in flies, 11.0% (11/100) in chicken meat, 8.9% (8/90) in chicken cloacal, 8.0% (4/50) in chicken caeca, and 7.5% (4/53) in wastewater. MCRPEC was significantly more resistant to co-amoxiclav, sulfamethoxazole-trimethoprim, levofloxacin, ciprofloxacin, and gentamicin than MCRNEC(p < 0.05). Genomic analysis revealed the distribution of MCRPEC among diverse clonal types. The putative plasmid Inc types associated withmcr-1were IncX4, IncHI2, IncP1, IncI2, and IncFIA, and those associated withmcr-3were IncFII, IncFIA, IncFIB, IncP1, and IncR. Recovery of highly similar plasmids from both flies and other sampling sectors implied the role of flies in the dissemination ofmcr-1. mcr-positive plasmids were shown to be conjugative, and a significantly high transfer rate into a hypervirulent clone ST1193 was observed. Plasmids containingmcrirrespective of Inc type were highly stable and invariably did not exert a fitness effect upon introduction into a new host. These findings signify the urgent need for a standard infection control program to radically decontaminate the source of resistance.
ISSN:2095-8099
DOI:10.1016/j.eng.2022.01.013