The in vitro effect of human polymorphonuclear leukocyte azurophil granule components on natural killer cell cytotoxicity
We previously demonstrated that human polymorphonuclear leukocyte (PMN) secretions are capable of activating and inhibiting natural killer cell (NK) cytotoxicity depending on the eliciting PMN stimulus. Serum-opsonized zymosan induced PMN to secrete substances that enhanced NK activity in vitro. Ser...
Uložené v:
| Vydané v: | Oral microbiology and immunology Ročník 9; číslo 3; s. 186 |
|---|---|
| Hlavní autori: | , , |
| Médium: | Journal Article |
| Jazyk: | English |
| Vydavateľské údaje: |
Denmark
01.06.1994
|
| Predmet: | |
| ISSN: | 0902-0055 |
| On-line prístup: | Zistit podrobnosti o prístupe |
| Tagy: |
Pridať tag
Žiadne tagy, Buďte prvý, kto otaguje tento záznam!
|
| Abstract | We previously demonstrated that human polymorphonuclear leukocyte (PMN) secretions are capable of activating and inhibiting natural killer cell (NK) cytotoxicity depending on the eliciting PMN stimulus. Serum-opsonized zymosan induced PMN to secrete substances that enhanced NK activity in vitro. Serum-opsonized zymosan stimulates the release of PMN azurophil granules, which contain both human neutrophil peptides (HNPs) and neutral serine proteases (NSPs). When HNPs and NSPs were tested for their ability to activate NK cells in peripheral blood lymphocytes, all but cathepsin G consistently enhanced cytotoxicity above control values. HNP-induced cytotoxicity was significantly enhanced within 12 h, peaking at approximately 24 h. Of the HNPs, HNP-1 was the most potent activator, enhancing NK activity at 1.25 micrograms/ml. Interleukin-2 and interferon-gamma were not involved in this activational process, as antibodies to interleukin-2 and interferon-gamma did not block activation by HNPs and NSPs, and interleukin-2 receptor expression was unaltered after 24 h of incubation. Enzymatically inactivated elastase and cathepsin G produced equivalent activational effects to their active counterparts. Antisera to elastase and cathepsin G decreased but did not eliminate NK activation over untreated peripheral blood lymphocytes. These data suggest that certain PMN azurophil granule components, including HNPs and NSPs directly increase the cytotoxic activity of NK cells. |
|---|---|
| AbstractList | We previously demonstrated that human polymorphonuclear leukocyte (PMN) secretions are capable of activating and inhibiting natural killer cell (NK) cytotoxicity depending on the eliciting PMN stimulus. Serum-opsonized zymosan induced PMN to secrete substances that enhanced NK activity in vitro. Serum-opsonized zymosan stimulates the release of PMN azurophil granules, which contain both human neutrophil peptides (HNPs) and neutral serine proteases (NSPs). When HNPs and NSPs were tested for their ability to activate NK cells in peripheral blood lymphocytes, all but cathepsin G consistently enhanced cytotoxicity above control values. HNP-induced cytotoxicity was significantly enhanced within 12 h, peaking at approximately 24 h. Of the HNPs, HNP-1 was the most potent activator, enhancing NK activity at 1.25 micrograms/ml. Interleukin-2 and interferon-gamma were not involved in this activational process, as antibodies to interleukin-2 and interferon-gamma did not block activation by HNPs and NSPs, and interleukin-2 receptor expression was unaltered after 24 h of incubation. Enzymatically inactivated elastase and cathepsin G produced equivalent activational effects to their active counterparts. Antisera to elastase and cathepsin G decreased but did not eliminate NK activation over untreated peripheral blood lymphocytes. These data suggest that certain PMN azurophil granule components, including HNPs and NSPs directly increase the cytotoxic activity of NK cells.We previously demonstrated that human polymorphonuclear leukocyte (PMN) secretions are capable of activating and inhibiting natural killer cell (NK) cytotoxicity depending on the eliciting PMN stimulus. Serum-opsonized zymosan induced PMN to secrete substances that enhanced NK activity in vitro. Serum-opsonized zymosan stimulates the release of PMN azurophil granules, which contain both human neutrophil peptides (HNPs) and neutral serine proteases (NSPs). When HNPs and NSPs were tested for their ability to activate NK cells in peripheral blood lymphocytes, all but cathepsin G consistently enhanced cytotoxicity above control values. HNP-induced cytotoxicity was significantly enhanced within 12 h, peaking at approximately 24 h. Of the HNPs, HNP-1 was the most potent activator, enhancing NK activity at 1.25 micrograms/ml. Interleukin-2 and interferon-gamma were not involved in this activational process, as antibodies to interleukin-2 and interferon-gamma did not block activation by HNPs and NSPs, and interleukin-2 receptor expression was unaltered after 24 h of incubation. Enzymatically inactivated elastase and cathepsin G produced equivalent activational effects to their active counterparts. Antisera to elastase and cathepsin G decreased but did not eliminate NK activation over untreated peripheral blood lymphocytes. These data suggest that certain PMN azurophil granule components, including HNPs and NSPs directly increase the cytotoxic activity of NK cells. We previously demonstrated that human polymorphonuclear leukocyte (PMN) secretions are capable of activating and inhibiting natural killer cell (NK) cytotoxicity depending on the eliciting PMN stimulus. Serum-opsonized zymosan induced PMN to secrete substances that enhanced NK activity in vitro. Serum-opsonized zymosan stimulates the release of PMN azurophil granules, which contain both human neutrophil peptides (HNPs) and neutral serine proteases (NSPs). When HNPs and NSPs were tested for their ability to activate NK cells in peripheral blood lymphocytes, all but cathepsin G consistently enhanced cytotoxicity above control values. HNP-induced cytotoxicity was significantly enhanced within 12 h, peaking at approximately 24 h. Of the HNPs, HNP-1 was the most potent activator, enhancing NK activity at 1.25 micrograms/ml. Interleukin-2 and interferon-gamma were not involved in this activational process, as antibodies to interleukin-2 and interferon-gamma did not block activation by HNPs and NSPs, and interleukin-2 receptor expression was unaltered after 24 h of incubation. Enzymatically inactivated elastase and cathepsin G produced equivalent activational effects to their active counterparts. Antisera to elastase and cathepsin G decreased but did not eliminate NK activation over untreated peripheral blood lymphocytes. These data suggest that certain PMN azurophil granule components, including HNPs and NSPs directly increase the cytotoxic activity of NK cells. |
| Author | Lindemann, R A Lala, A Miyasaki, K T |
| Author_xml | – sequence: 1 givenname: R A surname: Lindemann fullname: Lindemann, R A organization: Section of Oral Biology, Dental Research Institute, UCLA School of Dentistry – sequence: 2 givenname: A surname: Lala fullname: Lala, A – sequence: 3 givenname: K T surname: Miyasaki fullname: Miyasaki, K T |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/7936726$$D View this record in MEDLINE/PubMed |
| BookMark | eNotUMtOwzAQ9KGoQOETkCwO3BLs2InjI6p4SZW4FIlb5ThrmuLYwbER4esJontZrWZ2NDPnaOG8A4SuKcnpPLeHnDIpM0aKt5xKyfPYEEJKkX8v0BmRpMjmqzxF5-N4-AMqKZdoKSSrRFGdoWm7B9w5_NXF4DEYAzpib_A-9crhwdup92HYe5e0BRWwhfTh9RQBq58U_LDvLH4PyiULWPt-mM25OGLvsFMxBWXxR2ctBKzBWjw_-ui_O93F6QKdGGVHuDzuFXp9uN-un7LNy-Pz-m6Tac6qKhO6bYwqW6olqym0pGg4J3XdcCZI3dC2NBTEnMswYFwY4GXT1jUnwA2nghQrdPOvOwT_mWCMu74b_9woBz6NO1GJirOSz8SrIzE1PbS7IXS9CtPu2FXxC5zQcUM |
| CitedBy_id | crossref_primary_10_3389_fimmu_2022_894021 crossref_primary_10_1002_btm2_10704 crossref_primary_10_1189_jlb_0510250 crossref_primary_10_1182_blood_2021013233 crossref_primary_10_1074_jbc_M010279200 crossref_primary_10_1111_sji_12705 crossref_primary_10_3109_14767050902994796 crossref_primary_10_1189_jlb_3AB0316_140RR |
| ContentType | Journal Article |
| DBID | CGR CUY CVF ECM EIF NPM 7X8 |
| DOI | 10.1111/j.1399-302X.1994.tb00057.x |
| DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic |
| DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic |
| DatabaseTitleList | MEDLINE - Academic MEDLINE |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: 7X8 name: MEDLINE - Academic url: https://search.proquest.com/medline sourceTypes: Aggregation Database |
| DeliveryMethod | no_fulltext_linktorsrc |
| Discipline | Medicine Biology Dentistry |
| ExternalDocumentID | 7936726 |
| Genre | Research Support, U.S. Gov't, P.H.S Journal Article |
| GrantInformation_xml | – fundername: NIDCR NIH HHS grantid: DE-00292 – fundername: NIDCR NIH HHS grantid: DE-09174-03 |
| GroupedDBID | .GA .Y3 10A 123 1OB 1OC 29N 31~ 34H 4.4 51W 51X 52N 52O 52P 52S 52T 52W 52X 53G 5HH 5LA 66C 7PT 8-1 8-4 8-5 930 A03 AAEVG AAHHS AAHQN AAIPD AAKAS AAMNL AANHP AANLZ AAWTL AAXRX AAYCA AAZKR ABCQN ABCUV ABEML ABJNI ABQWH ABXGK ACAHQ ACBWZ ACCFJ ACCZN ACGFS ACMXC ACPOU ACRPL ACSCC ACXBN ACXQS ACYXJ ADBTR ADEOM ADIZJ ADMGS ADNMO ADOZA ADZCM AEEZP AEIGN AEIMD AEQDE AEUYR AFBPY AFEBI AFFNX AFFPM AFGKR AFWVQ AFZJQ AHBTC AHEFC AIACR AITYG AIURR AIWBW AJBDE ALMA_UNASSIGNED_HOLDINGS ALUQN AMBMR AMYDB ATUGU AZFZN BDRZF BRXPI BY8 C45 CAG CGR CO8 COF CS3 CUY CVF CWXXS D-F DCZOG DRFUL DRMAN DRSTM DU5 EBS ECM EIF EJD F00 F01 F04 F5P FEDTE FZ0 G-S GODZA HGLYW HVGLF HZI IHE LATKE LEEKS LH4 LITHE LOXES LP6 LP7 LUTES LW6 LYRES MEWTI MM. MRFUL MRMAN MRSTM MSFUL MSMAN MSSTM MXFUL MXMAN MXSTM NPM OIG OVD P4D PALCI QB0 RIWAO RJQFR ROL SAMSI SUPJJ TEORI UB1 WIH WIJ WIK WQJ WXSBR XG1 YFH ZGI 7X8 AGQPQ AIQQE |
| ID | FETCH-LOGICAL-c4366-7cdbfa5d1c9381ed02b44088b43708b1d5f1e7056f3e347fe45bd8840e4f41702 |
| IEDL.DBID | 7X8 |
| ISICitedReferencesCount | 10 |
| ISICitedReferencesURI | http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=10_1111_j_1399_302X_1994_tb00057_x&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| ISSN | 0902-0055 |
| IngestDate | Sun Sep 28 07:30:22 EDT 2025 Thu Apr 03 07:02:22 EDT 2025 |
| IsPeerReviewed | false |
| IsScholarly | false |
| Issue | 3 |
| Language | English |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c4366-7cdbfa5d1c9381ed02b44088b43708b1d5f1e7056f3e347fe45bd8840e4f41702 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| PMID | 7936726 |
| PQID | 76764354 |
| PQPubID | 23479 |
| ParticipantIDs | proquest_miscellaneous_76764354 pubmed_primary_7936726 |
| PublicationCentury | 1900 |
| PublicationDate | June 1994 |
| PublicationDateYYYYMMDD | 1994-06-01 |
| PublicationDate_xml | – month: 06 year: 1994 text: June 1994 |
| PublicationDecade | 1990 |
| PublicationPlace | Denmark |
| PublicationPlace_xml | – name: Denmark |
| PublicationTitle | Oral microbiology and immunology |
| PublicationTitleAlternate | Oral Microbiol Immunol |
| PublicationYear | 1994 |
| SSID | ssj0005699 |
| Score | 1.2460804 |
| Snippet | We previously demonstrated that human polymorphonuclear leukocyte (PMN) secretions are capable of activating and inhibiting natural killer cell (NK)... |
| SourceID | proquest pubmed |
| SourceType | Aggregation Database Index Database |
| StartPage | 186 |
| SubjectTerms | alpha-Defensins Animals Antimicrobial Cationic Peptides Blood Proteins - immunology Carrier Proteins Cathepsin G Cathepsins - immunology Cytoplasmic Granules - immunology Cytotoxicity, Immunologic - immunology Defensins Humans Killer Cells, Lymphokine-Activated - immunology Killer Cells, Natural - immunology Lymphocyte Activation Neutrophils - enzymology Neutrophils - immunology Neutrophils - metabolism Pancreatic Elastase - immunology Serine Endopeptidases - immunology Tumor Cells, Cultured |
| Title | The in vitro effect of human polymorphonuclear leukocyte azurophil granule components on natural killer cell cytotoxicity |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/7936726 https://www.proquest.com/docview/76764354 |
| Volume | 9 |
| WOSCitedRecordID | wos10_1111_j_1399_302X_1994_tb00057_x&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| hasFullText | |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV09b9swED0kcb6WNnFi5KNtOGQlIokUaQEBiqJN0MWGhxbwZlAU2Rp2RMexjTi_PneSjE5FhiyaROnAO_FOx8f3AK6tMlppI7jH2oHLTMQc00jBE5tJa5SSaV6LTeh-vzscZoMtuN2chSFY5WZNrBbqIljqkd_gEzF5pvLr7JGTZhTtrTYCGtvQEljIUEzr4T-u8FRlNdMeYWyjNG0oRxscD97PRZQ803E9WfVHsXKpCJD-U2hWCef-4_tMPYIPTaHJvtWRcQxbrmzDXi09uW7DwQ-CCZHSWxv2e80G-wmQjh0bl2w1XswDq8EeLHhWSfmxWZiuH8Kc4OxEg2zmbOqWk2DXC8fMC_X1_46n7A-mv-XUMUKrh5KAGiyUrGIQRYMm1eFDRhsGDAeGRXjGdy_Wp_D7_u7X95-8UWfgVgqluLZF7k1axDbDrO-KKMlJvbqbS6Gjbh4XqY-dRi944YTU3qHbiy7-TzrpZayjpAM7JVpxBswU2muZxyKWJDOeZJGQwmACdXFuVaTP4Woz0SOMfrLQlC4sn0abqT6HTu2r0awm6RjhuqN0oi7eHHoJhzU_MnVWPkHL42fvPsOuXaEX5l-qmMJrf9B7BdpX1sU |
| linkProvider | ProQuest |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=The+in+vitro+effect+of+human+polymorphonuclear+leukocyte+azurophil+granule+components+on+natural+killer+cell+cytotoxicity&rft.jtitle=Oral+microbiology+and+immunology&rft.au=Lindemann%2C+R+A&rft.au=Lala%2C+A&rft.au=Miyasaki%2C+K+T&rft.date=1994-06-01&rft.issn=0902-0055&rft.volume=9&rft.issue=3&rft.spage=186&rft_id=info:doi/10.1111%2Fj.1399-302x.1994.tb00057.x&rft.externalDBID=NO_FULL_TEXT |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0902-0055&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0902-0055&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0902-0055&client=summon |