Comparison of gut microbiota composition between laboratory‐bred marmosets (Callithrix jacchus) with chronic diarrhea and healthy animals using terminal restriction fragment length polymorphism analysis

ABSTRACT Chronic diarrhea in laboratory‐bred marmosets poses a serious health problem during experiments. Despite a growing demand for laboratory‐bred experimental marmosets, the mechanisms underlying the development of diarrhea and measures for its treatment and prevention remain unclear. To explor...

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Published in:Microbiology and immunology Vol. 62; no. 11; pp. 702 - 710
Main Authors: Shigeno, Yuko, Toyama, Mutsumi, Nakamura, Mutsumi, Niimi, Kimie, Takahashi, Eiki, Benno, Yoshimi
Format: Journal Article
Language:English
Published: Australia Wiley Subscription Services, Inc 01.11.2018
Subjects:
Animals
Animals, Laboratory - microbiology
Bacterial Typing Techniques
Base Sequence
Bifidobacterium - genetics
Bifidobacterium - isolation & purification
Callithrix - microbiology
Cluster Analysis
common marmoset
Diarrhea
Diarrhea - microbiology
Diarrhea - veterinary
DNA, Bacterial - genetics
Feces - microbiology
Female
Gastrointestinal Microbiome - genetics
Genes, Bacterial - genetics
gut microbiota
Intestinal microflora
Laboratories
Male
Microbiota
Monkey Diseases - microbiology
Phylogeny
Polymorphism
Polymorphism, Restriction Fragment Length
Real-Time Polymerase Chain Reaction - veterinary
Restriction fragment length polymorphism
RNA, Ribosomal, 16S - genetics
terminal restriction fragment length polymorphism analysis
terminal restriction fragment length polymorphism analysis
diarrhea
gut microbiota
common marmoset
ISSN:0385-5600, 1348-0421, 1348-0421
Online Access:Get full text
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Summary:ABSTRACT Chronic diarrhea in laboratory‐bred marmosets poses a serious health problem during experiments. Despite a growing demand for laboratory‐bred experimental marmosets, the mechanisms underlying the development of diarrhea and measures for its treatment and prevention remain unclear. To explore the factors affecting development of chronic diarrhea in laboratory‐bred marmosets, the gut microbiota composition (GMC) of 58 laboratory‐bred marmosets, including 19 animals with chronic diarrhea, was analyzed using terminal restriction fragment length polymorphism. We found that the GMCs in these animals cluster into two groups that differ significantly in rate of chronic diarrhea (56.5% in one group, Cluster 1, and 17.1% in Cluster 2). Additionally, a higher α‐diversity and a lower proportion of Bifidobacterium spp. according to quantitative PCR was found the animals in the Cluster 1 than in those in Cluster 2. Taken together, our findings indicate that there is a relationship between GMC and development of chronic diarrhea in laboratory‐bred marmosets. This is the first study to highlight the potential of assessing GMC in relation to development of chronic diarrhea in laboratory‐bred marmosets.
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ISSN:0385-5600
1348-0421
1348-0421
DOI:10.1111/1348-0421.12655