Effect of whole oral bacteria and extracted lipopolysaccharides on peripheral blood leukocyte interleukin-2 receptor expression

Expression of the interleukin-2 receptor (IL-2R) on T cells is the molecular mechanism that initiates the G0 to G1 transition and is the critical first step for T cell proliferation in response to antigen. The effect of whole periodontal bacteria and lipopolysaccharides (LPS) on peripheral blood mon...

Celý popis

Uložené v:
Podrobná bibliografia
Vydané v:Journal of periodontal research Ročník 30; číslo 4; s. 264
Hlavní autori: Lindemann, R A, Kjeldsen, M, Cabret, M
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: United States 01.07.1995
Predmet:
Aggregatibacter actinomycetemcomitans - immunology
Capnocytophaga - immunology
Cell Separation
Cells, Cultured
Culture Media, Conditioned - pharmacology
Flow Cytometry
Fusobacterium nucleatum - immunology
Gram-Negative Anaerobic Bacteria - immunology
Humans
Interferon-gamma - immunology
Leukocytes, Mononuclear - immunology
Leukocytes, Mononuclear - metabolism
Lipopolysaccharides - immunology
Lymphocyte Activation
Porphyromonas gingivalis - immunology
Receptors, Interleukin-2 - biosynthesis
Recombinant Proteins
T-Lymphocytes - immunology
T-Lymphocytes - metabolism
ISSN:0022-3484
On-line prístup:Zistit podrobnosti o prístupe
Tagy: Pridať tag
Žiadne tagy, Buďte prvý, kto otaguje tento záznam!
Popis
Shrnutí:Expression of the interleukin-2 receptor (IL-2R) on T cells is the molecular mechanism that initiates the G0 to G1 transition and is the critical first step for T cell proliferation in response to antigen. The effect of whole periodontal bacteria and lipopolysaccharides (LPS) on peripheral blood mononuclear cell (PBMC) IL-2R expression was examined in vitro. LPS induced a modest but significant increase in high affinity IL-2R alpha/beta (p55/p75 positive) expression on PBMC over untreated cells after 48 h culture. Addition of LPS to PBMC cultures depleted of monocytes had no effect on IL-2R expression compared to untreated cultures. Interleukin-1 (IL-1) caused a similar effect to LPS in 48 h PBMC cultures but IL-1 also increased high affinity IL-2R expression in cultures depleted of adherent mononuclear cells. When antibody to IL-1 was simultaneously added with LPS to PBMC cultures, the high affinity IL-2R inductive effect was reversed at 48 h, suggesting that the LPS effect on PBMC IL-2R was indirect, via monocytes. Whole pathogenic oral bacteria cultured with PBMC at high (100:1), but not low (10:1) bacteria:PBMC ratios had a similar effect to LPS, inducing high affinity IL-2R expression at 48 h. Increases in soluble IL-2R alpha were also measured in supernatants of PBMC incubated with periodontal bacteria compared to untreated controls. In this system, a critical threshold of bacteria was required to activate PBMC perhaps related to the quantity of cell-surface LPS presented to adherent mononuclear cells.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0022-3484
DOI:10.1111/j.1600-0765.1995.tb02132.x