Optimization of extraction with salicylic acid, rheological behavior and antiproliferative activity of pectin from Citrus sinensis peels

A Box-Behnken design was used to optimize extraction temperature, extraction time and concentration of the salicylic acid to obtain a maximum polysaccharide yield from Citrus sinensis peels. The optimal settings were: extraction time 3 h, extraction temperature 80 °C and concentration of the salicyl...

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Veröffentlicht in:	International journal of biological macromolecules Jg. 159; S. 547 - 556
Hauptverfasser:	Haj Romdhane, Mariem, Beltifa, Asma, Mzoughi, Zeineb, Rihouey, Christophe, Ben Mansour, Hedi, Majdoub, Hatem, Le Cerf, Didier
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	Netherlands Elsevier B.V 15.09.2020 Elsevier
Schlagworte:	Antineoplastic Agents, Phytogenic - chemistry Antineoplastic Agents, Phytogenic - isolation & purification Antineoplastic Agents, Phytogenic - pharmacology Antiproliferative activity arabinose Box-Behnken design Carbohydrates - chemistry Carbohydrates - isolation & purification Cell Line, Tumor Cell Proliferation - drug effects Chemical Sciences Chromatography, Gel Citrus sinensis Citrus sinensis - chemistry esterification experimental design galactose glucose Humans hydrolysis mannose Material chemistry Molecular Weight Pectin pectins Pectins - chemistry Pectins - isolation & purification Pectins - pharmacology Plant Proteins - chemistry Plant Proteins - isolation & purification Polymers Polysaccharides - chemistry Polysaccharides - isolation & purification rhamnose Rheological behavior Rheology Salicylic acid Salicylic Acid - chemistry Salicylic Acid - isolation & purification Solubility Spectroscopy, Fourier Transform Infrared temperature uronic acids viscosity xylose Salicylic acid Pectin Rheological behavior Antiproliferative activity Box-Behnken design
ISSN:	0141-8130, 1879-0003, 1879-0003
Online-Zugang:	Volltext
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Zusammenfassung:	A Box-Behnken design was used to optimize extraction temperature, extraction time and concentration of the salicylic acid to obtain a maximum polysaccharide yield from Citrus sinensis peels. The optimal settings were: extraction time 3 h, extraction temperature 80 °C and concentration of the salicylic acid 1.5%. Under these conditions, the experimental yield and uronic acid content were 11.74% and 66.9% respectively. Preliminary characterization was performed via FT-IR, SEC/MALS/VD/DRI and GC–MS after hydrolysis. SEC analysis showed that the extracted polysaccharide had a weight average molar mass of 350 kDa and an intrinsic viscosity of 640 mL/g. The GC–MS results revealed that the extracted polysaccharide was composed of arabinose 56.7%, galactose 17.8%, xylose 13.8%, rhamnose 5.1%, mannose 2.5% and glucose 1.5% suggested a rhamnogalacturonan pectin type I with a degree of esterification of 50.9% (IRTF). The flow curve and the dynamic frequency sweep were obtained at 10, 20, 30 and 40 g/L in water and at 30 g/L in presence of CaCl2 or NaCl at 1 mol/L. The solutions showed shear-thinning behavior fitted with Ostwald-De Waele model, except 10 g/L with a Newtonian behavior. The apparent viscosity and, the G’ and G” moduli increase with PACO concentration in agreement with a slow-down of the dynamic chain. In the presence of CaCl2 or NaCl the reduction of electrostatic repulsions between pectin chains decreases the rheological parameters. The effect is less sensitive with CaCl2 due to intermolecular interactions. The antiproliferative activity of the extracted pectin on human Caco-2 and Hep-2 cells was very interesting with an IC50 1.4 and 1.8 μg/mL respectively.
Bibliographie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0141-8130 1879-0003 1879-0003
DOI:	10.1016/j.ijbiomac.2020.05.125